The Effect of Vitamin D3 on Skeletal Muscle in Rat after Ischemia Reperfusion Injury: Preliminary Report

Abstract

Objective: Vitamin D is a vitamin that has gained popularity in recent years and has an anti-inflammatory and immunmodular effect. In the literature review we conducted, there was no study investigating the effect of vitamin D to prevent ischemia reperfusion (I/R) injury in skeletal muscle. The aim of this study was to investigate the effect of vitamin D on skeletal muscle in I/R injury.

Methods: Six rats were used in each group including ischemia group (group I) and experimental group (group D given vitamin D). In the histopathological examination, inflammation and apoptosis levels were studied in gastrocnemius muscle. In biochemical analysis, total oxidative stress and total antioxidant and catalase levels were evaluated in anterior tibialis muscle.

Results: The number of apoptotic cells in group D were found significantly lower than group I. In the terms of total antioxidants and catalase levels, there were significant difference between group D and group I.

Conclusion: Our results support the hypothesis that vitamin D is an agent that can be used to prevent I/R injury.