Person: KOÇYİĞİT, ABDÜRRAHİM
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Publication Oleanolik Asit Türevlerinin Sentezi ve Sitotoksik Aktivitelerinin Araştırılması(2017-04-02) TUNCAY, SALİH; ŞENOL, HALİL; GÜLER, ERAY METİN; ÖCAL, NÜKET; SEÇEN, HASAN; KOÇYİĞİT, ABDÜRRAHİM; TOPÇU, GÜLAÇTI; ŞENOL, HALIL; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; TOPÇU, GÜLAÇTIPublication Antimicrobial peptides cathelicidin (LL-37) ve hum beta 2 defensin levels of pediatric patients with bronchiolitis obliterans(2014-09-01T00:00:00Z) Gedik, Ahmet Hakan; Cakir, Erkan; Gokdemir, Yasemin; Uyan, Zeynep Seda; KOÇYİĞİT, ABDÜRRAHİM; Torun, Emel; Karadag, Bulent; Ersu, Retika; Karakoc, Fazilet; Hakan, Ahmet; KOÇYİĞİT, ABDÜRRAHİMPublication Investigation of N-terminal Pro Brain Natriuretic Peptide, Oxidative Stress, and Thiol Disulfide Homeostasis in Vitiligo Patients(2020-03-17T00:00:00Z) Kutlu, Büşra; EMİROĞLU, NAZAN; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; EMİROĞLU, NAZAN; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİMPublication Sperm DNA damage and seminal oxidative status after shock-wave lithotripsy for distal ureteral stones(2011-11-01T00:00:00Z) Gulum, Mehmet; Yeni, Ercan; Kocyigit, ABDÜRRAHİM; Taskin, Abdullah; Savas, Murat; Ciftci, Halil; Altunkol, Adem; KOÇYİĞİT, ABDÜRRAHİMObjective: To investigate the relationship between level of sperm DNA damage, seminal oxidative status, and shock-wave lithotripsy (SWL) for distal ureteral stones.Publication Intracellular pH-mediated induction of apoptosis in HeLa cells by a sulfonamide carbonic anhydrase inhibitor(2022-03-15T00:00:00Z) Koyuncu, Ismail; Temiz, Ebru; Durgun, Mustafa; KOÇYİĞİT, ABDÜRRAHİM; Yuksekdag, Ozgur; Supuran, Claudiu T.; KOÇYİĞİT, ABDÜRRAHİM© 2021Carbonic anhydrase IX (CAIX) is a hypoxia-associated transmembrane protein that is critical in the survival of cells. Because CAIX has a key role in pH regulation, its therapeutic effects have been heavily studied by different research laboratories. This study aims to investigate how a synthetic CAIX inhibitor triggers apoptosis in a cancer cell line, HeLa. In this regard, we investigated the effects of the compound I, synthesized as a CAIX inhibitor, on the survival of cancer cells. The compound I inhibited the proliferation of the CAIX+ HeLa cells, kept the cells in G0/G1 phase (74.7%) and altered the cells morphologies (AO/EtBr staining) and the nuclear structure (γ-H2AX staining). CAIX inhibition triggered apoptosis in HeLa cells with a rate of 47.4%. According to the expression of mediator genes (CASP-3, -8, -9, BAX, BCL-2, BECLIN, LC3), the both death pathways were activated in HeLa cells with the inhibition of CAIX with the compound I. The compound I was also determined to affect the genes and proteins that have a critical role in the regulation of apoptotic pathways (pro casp-3, cleaved casp-3, -8, -9, cleaved PARP and CAIX). Furthermore, CAIX inhibition caused changes in pH balance, disruption in organelle integrity of mitochondria, and increase intracellular reactive oxygen level of HeLa cells. Taken together, our findings suggest that CAIX inhibition has a potential in cancer treatment, and the compound I, a CAIX inhibitor, could be a promising therapeutic strategy in the treatment of aggressive tumours.Publication Paraoxonase-1 activity and oxidative status in patients with knee osteoarthritis and their relationship with radiological and clinical parameters(2012-09-01T00:00:00Z) Erturk, Cemil; Altay, Mehmet Akif; Selek, Sahbettin; Kocyigit, ABDÜRRAHİM; SELEK, ŞAHABETTİN; KOÇYİĞİT, ABDÜRRAHİMBackground. The aim of this study was to investigate serum paraoxonase-1 (PON1) activity and oxidative/anti-oxidative status in knee osteoarthritis (OA), and evaluate their relationship using radiological and clinical parameters. Materials and methods. The study population comprised 127 patients with knee OA and 107 healthy volunteers. Patients with knee OA were divided into four subgroups according to the Kellgren-Lawrence (K&L) grading scale. In addition, each patient was clinically evaluated by the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Serum PON1 activity was measured spectrophotometrically. Oxidative status was assessed by measuring serum lipid hydroperoxide (LOOH) and total oxidant status (TOS). Anti-oxidative status was assessed by measuring serum free sulfydryl groups (-SH = total thiol) and total antioxidant capacity (TAC). Oxidative stress index (OSI) was calculated. Lipid parameters were determined by routine laboratory methods. Results. Serum PON1 activity was significantly lower in the knee OA group compared to the control group (p 0.05). The lowest and highest mean serum PON1 activities were detected in patients with grades 4 and 1, respectively (ANOVA p < 0.001). In multiple regression analysis, WOMAC score was independently associated with serum PON1 activity (beta = -0.248, p = 0.027). Conclusions. Decreased serum PON1 activity and elevated LOOH, TOS, and OSI levels may be associated with knee OA, and serum PON1 activity may be a useful adjunctive indicator of the severity of knee OA for follow-up.Publication BÜTÜNCÜL TIBBA GENEL BAKIŞ BİLİMSEL VERİLER IŞIĞINDA AROMATERAPİ(Selen Yayınları, 2022-12-01) Koçyiğit A. (Editör); KOÇYİĞİT, ABDÜRRAHİMPublication AURALI VE AURASIZ MİGREN ATAĞINDA TİYOL/DİSÜLFİD HOMEOSTAZI VE OKSİDATİF STRES BULGULARI(2019-11-15T00:00:00Z) UZUN, MUSTAFA; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; USLU, FERDA; KOÇYİĞİT, ABDÜRRAHİM; USLU, FERDAPublication Investigation of Ficus Carica Plantae-Cytotoxic, Genotoxic, Apoptotic, Antineoplastic, Anti-inflammatory, and Autophagic Effects on Breast Cancer Cells.(2021-04-01T00:00:00Z) Koçyiğit, Abdürrahim; KOÇYİĞİT, ABDÜRRAHİMPublication A Study on Thymoquinone: Antioxidant Capacity and Anticancer Activities in LoVo Colorectal Cancer Cells(2024-02-02) Bozali K.; Metin Guler E.; KOÇYİĞİT A.; KOÇYİĞİT, ABDÜRRAHİMThymoquinone has antioxidant and anticancer effects. This study investigates the cytotoxic, genotoxic, and apoptotic effects of black seed and its active ingredient, thymoquinone on colorectal cancer cells. The antioxidant content of Black seed methanolic extracts (BSME) with different concentrations (50, 500 and 1000 mu g/mL) were determined by the photometric methods. The reactive oxygen production (iROS) of BSME and thymoquinone on colorectal cancer cells (LoVo) and normal epithelial cells (CCD18Co) were analyzed by the fluorometric methods. A luminometric glutathione kit was employed to observe the changes in intracellular glutathione (GSH) levels. Cytotoxicity was determined by the ATP method, genotoxicity was determined by Comet Assay, and the apoptosis was identified by the Acridine Orange/Ethidium Bromide (AO/EB) double dye method. The cytotoxicity was increased by BSME and thymoquinone in LoVo cells in a dose-dependent manner (p<0.001). BSME and thymoquinone also increased iROS, and induced apoptosis and DNA damage (p<0.001). High doses of BSME and thymoquinone on cancer and healthy cells have cytotoxic, genotoxic and apoptotic effects with pro-oxidant effects. Colorectal cancer cells are more sensitive than healthy cells.