Yayınlar - Eserler

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12645/130

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Long-Term Disease Course of Pontocerebellar Hypoplasia Type 10.
(2024-06-06) Guler S.; Aslanger A. D.; Uygur Sahin T.; Alkan A.; Yalcinkaya C.; Saltik S.; Yesil G.; ALKAN, ALPAY
Cutaneous necrosis following endovascular embolization by using ethylene-vinyl alcohol copolymer for arterio-venous malformations
(2024-02-01) EVİN N.; VURAL Y.; Ergün S. S.; EVİN, NUH; VURAL, YASİR; ERGÜN, SELMA
Assessment of drug-induced electrolyte disorders in intensive care units: a multicenter observational study
(2024-01-01) Ayhan Y. E.; İLERLER E. E.; Sosyal D.; BEKTAY M. Y.; KARAKURT S.; DAŞKAYA H.; KARAASLAN K.; SANCAR M.; DAŞKAYA, HAYRETTİN; KARAASLAN, KAZıM
Objective: Electrolyte disorder (ED) is frequently encountered critically ill patients during admission or admission to the intensive care unit (ICU). This study aimed to determine the frequency of ED encountered in ICU patients to evaluate the relationship of ED with drugs. Methods: This prospective, multicenter study was conducted in the medical and anesthesiology ICUs of two training and research hospitals and included patients with at least one ED during admission or hospitalization in the ICUs. The relationship between ED and the drug was evaluated by calculating the logistic probabilistic method scale (LPMS) and the expert panel’s evaluation. The correlation between EDs and LPMS was determined using Kendal tau. A binary logistic regression model was preferred in the analysis of factors related to ED. Statistical significance was set as p < 0.05. Results: A total of 117 patients were included in the study. A total of 165 EDs were detected, including at least one in 88 (75.2%) patients. According to the expert panel, 61 (21.7%) of EDs were drug-related, whereas according to the LPMS, 111 (39.6%) (p < 0.001). Mortality (50% vs. 13.7%) and mechanical ventilation rates (52.2% vs. 17.2%) were significantly higher in patients with ED (p < 0.001). Patients with ED had 8.352 times higher odds of exhibiting mortality (OR: 8.352, %95 CI: 1.598–43.648, p: 0.012) and need mechanical ventilation with higher odds of 3.229 (OR: 3.229 95% CI: 0.815–12.787 p: 0.045). Patient who required enteral or parenteral feeding were associated with an increased likelihood of exhibiting ED (respectively OR: 30.057, %95 CI: 2.265–398.892, p: 0.01, OR: 5.537, %95 CI: 1.406–21.800, p: 0.014). Conclusion: EDs are very common in the ICU. Dysnatremia was detected more commonly in other EDs. It has also been found that patients with ED are more often under mechanical ventilation, have more prolonged hospitalizations, and have higher mortality rates than patients without ED. The suitability of LPMS for assessing ED-drug relationships in the ICU context is questioned.
Harnessing autophagy: A potential breakthrough in digestive disease treatment
(2024-06-01) Eşrefoğlu M.; EŞREFOĞLU, MUKADDES
Autophagy, a conserved cellular degradation process, is crucial for variouscellular processes such as immune responses, inflammation, metabolic andoxidative stress adaptation, cell proliferation, development, and tissue repair andremodeling. Dysregulation of autophagy is suspected in numerous diseases,including cancer, neurodegenerative diseases, digestive disorders, metabolicsyndromes, and infectious and inflammatory diseases. If autophagy is disrupted,for example, this can have serious consequences and lead to chronic inflammationand tissue damage, as occurs in diseases such as Chron\"s disease and ulcerativecolitis. On the other hand, the influence of autophagy on the development andprogression of cancer is not clear. Autophagy can both suppress and promote theprogression and metastasis of cancer at various stages. From inflammatory boweldiseases to gastrointestinal cancer, researchers are discovering the intricate role ofautophagy in maintaining gut health and its potential as a therapeutic target.Researchers should carefully consider the nature and progression of diseases suchas cancer when trying to determine whether inhibiting or stimulating autophagyis likely to be beneficial. Multidisciplinary approaches that combine cutting-edgeresearch with clinical expertise are key to unlocking the full therapeutic potentialof autophagy in digestive diseases
Phytoactive Molecules and Nanodelivery Approaches for Breast Cancer Treatment: Current and Future Perspectives
(2024-06-07) ALĞIN YAPAR E.; ÖZDEMİR M. N.; Cavalu S.; Dagistan O. A.; Ozsoy Y.; KARTAL M.; KARTAL, MURAT
One of the most common malignancies in women, breast cancer accounts for nearly 25% of all cancer cases. Breast cancer is a diverse cancer form that exhibits variability in both morphology and molecular characteristics and is linked to numerous risk factors. Although various approaches and research are ongoing in the treatment and prevention of breast cancer, medication resistance in the current breast cancer treatment contributes to the disease\"s relapse and recurrence. Phytoactive molecules are the subject of growing research in both breast cancer prevention and treatment, but currently used conventional medicines and techniques limit their application. In recent years, significant advancements have been made in the field of nanotechnology, which has proven to be essential in the fight against drug resistance. The transport of synthetic and natural anticancer molecules via nanocarriers has recently been added to breast cancer therapy, greatly alleviating the constraints of the current approach. In light of these developments, interest in nano-delivery studies of phytoactive molecules has also increased. In this review, research of phytoactive molecules for breast cancers along with their clinical studies and nanoformulations, was presented from current and future perspectives.
Effects of Platelet-Rich Plasma and Concentrated Growth Factor on Viability of Ultra-Diced Cartilage Grafts in a Rabbit Model.
(2024-06-10) Kulaksiz Y.; Yenigün A.; Şerif Aydin M.; Doğan R.; Tuğrul S.; Özturan O.; YENİGÜN, ALPER; DOĞAN, REMZI
Investigation of the Anti-inflammatory Effect of Liquidambar orientalis Leaf Extract on RAW 264.7 Macrophage Cells
(2024-03-02) Şahin H.; Özman Z.; Koçyiğit A.; ÖZMAN, ZEYNEP; KOÇYİĞİT, ABDÜRRAHİM
Diabetes Mellitus ile Aralıklı Oruç Diyeti Etkileşimi
(BİDGE Yayınları, 2024-06-01) Buz A.; Güneş Bayır A.; GÜNEŞ BAYIR, AYŞE
Investigating the Antioxidant Capacity of Lunasin Expressed in Aspergillus oryzae
(2024-01-01) KARAMAN E.; Albayrak C.; UYSAL S.; KARAMAN, ELİF; ALBAYRAK, CEM; UYSAL, SERDAR
Objective: Lunasin is a bioactive protein that possesses anti-carcinogenic, anti-inflammatory, and antioxidant properties. Traditional isolation methods are resource-intensive, and chemical synthesis faces cost and environmental issues. This study aims to achieve cost-effective lunasin expression in Aspergillus oryzae with a focus on exploring its antioxidant properties in vitro. Materials and Methods: The expression vector carrying four lunasin sequences fused with amylase and an 8xHis-tag was introduced into pyrG auxotrophic A. oryzae. Subsequently, the recombinant protein was purified using metal affinity chromatography. The study uses sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), western blot analyses, and size-exclusion chromatography to evaluate the composition and purity of the protein, a linoleic acid assay to demonstrate the inhibitory effect on lipid peroxidation, and the 2,2\"-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid] ABTS) assay to evaluate the radical scavenging activity. Results: SDS-PAGE and western blot analyses confirmed sustained lunasin expression in A. oryzae, appearing in both fusion and non-fusion forms. Yields were 5.8 mg/L for non-fusion and 4 mg/L for fusion lunasin expression. Moreover, 0.1 μM non-fusion lunasin surpassed α-tocopherol and butylated hydroxyanisole (BHA; p < 0.05) in reducing lipid peroxidation at 4 and 72 h. Unlike the fusion lunasin, the non-fusion lunasin displayed concentration- and time-independent inhibitory effects on linoleic acid peroxidation as well as significant ABTS scavenging activity (p < 0.05). Conclusion: The study has shown for the first time A. oryzae to efficiently express and secrete both fusion and non-fusion lunasin proteins in a soluble form, with the non-fusion lunasin exhibiting superior antioxidant effectiveness compared to the fusion lunasin. The findings underscore A. oryzae\"s potential as a promising host for producing functional lunasin with antioxidant properties, opening avenues for broader applications in biotechnology and bioactive peptides.
Comparative cell viability of dentin-bonding adhesive systems on human dental pulp stem cells: time-dependent analysis
(2024-12-01) Kazak M.; Sarialioglu Gungor A.; ÖZMAN Z.; Donmez N.; ÖZMAN, ZEYNEP
Background: Restorative materials are in prolonged contact with living tissues such as oral mucosa, dentin, pulp, periodontal, and periapical tissues. Therefore, the potentially harmful effects of these materials and their components on oral tissues should be evaluated before clinical use. This study aimed to compare the cell viability of different adhesive systems (ASs) on human dental pulp stem cells (hDPSCs). Methods: Three ASs that combining methacryloyloxydecyl dihydrogen phosphate (MDP) monomer with new hydrophilic amide monomers [Clearfil Universal Bond Quick(CUBQ), Kuraray Noritake], self-reinforcing 3D monomer [Bond Force II(BFII), Tokuyama)], and dual-cure property [Futurabond DC(FBDC), VOCO] were used. Three (n = 3) samples were prepared for each group. Dental pulp stem cells were isolated from ten patients' extracted third molar teeth. Samples were incubated in Dulbecco's modified Eagle's medium (DMEM) for 24 h (h), 72 h, and 7 days (d) to obtain extracts. For the control group, cells were cultured without DBA samples. Cell viability of ASs extracts was measured using a cell proliferation detection kit (WST-1, Roche). Statistical analysis was performed using two-way ANOVA and post-hoc (Duncan) tests (p < 0.05). Results: At 24 and 72 h statistically significant differences were determined between control and BFII, control and FBDC groups (p < 0.05), while no differences between control and CUBQ groups (p > 0.05). On the 7th d, statistically significant differences were found between the control and experimental groups (p < 0.05), while no differences between experimental groups (p > 0.05). A statistically significant difference was detected for the BFII group over the three-time interval (p < 0.05). The lowest cell viability was observed for the FBDC group at 24 h, and the difference was statistically significant when compared with 72 h and 7th d (p < 0.05). Conclusion: All ASs showed different cell viability values at various exposure times. It should be taken into consideration that pH values, as well as the contents of ASs, have a significant effect on the cell viability.
Effect of Vitamin D Deficiency on Periprosthetic Joint Infection and Complications After Primary Total Joint Arthroplasty
(2024-01-01) BİRİNCİ M.; HAKYEMEZ Ö. S.; GEÇKALAN M. A.; Mutlu M.; YILDIZ F.; Bilgen Ö. F.; AZBOY İ.; GEÇKALAN, MUHAMMED ALİ; YILDIZ, FATİH
Background: Vitamin D deficiency is a global problem, and 13 to 75% of patients undergoing total joint arthroplasty (TJA) have vitamin D deficiency. Several studies have shown that low preoperative vitamin D levels may increase the risk of postoperative complications, including periprosthetic joint infection (PJI), in patients undergoing primary TJA. Most of the studies are underpowered. This study aimed to investigate the relationship between vitamin D deficiency and surgical and medical complications after primary TJA, with a specific focus on PJI. Methods: Prospectively collected institutional multicenter arthroplasty databases were reviewed to identify patients who underwent primary total knee and hip arthroplasty. The study group was defined as patients whose vitamin D level is < 30 ng/dL and who received a single oral dose of 7.5 mg (300,000 IU) D3 within two weeks before index surgery (n = 488; mean age 63 years). Patients in the control group were those whose preoperative vitamin D levels were unknown and who did not receive vitamin D supplementation (n = 592, mean age 66). The groups were compared regarding 90-day medical and surgical complications, including PJI, mortality, and readmission rates. Results: The total number of complications (8.6 and 4.3%; respectively; P = .005), superficial wound infection (2.5 and 0.2%, respectively; P < .001), and postoperative cellulitis (2.2 and 0% respectively; P < .001) were statistically significantly higher in the patient group who did not receive vitamin D supplementation. However, 90-day mortality (P = .524), PJI (P = .23), and readmission rate (P = .683) were similar between the groups. Conclusions: This study demonstrated that preoperative optimization of vitamin D levels may be beneficial in reducing postoperative complications, including superficial wound infection and postoperative cellulitis. Administering an oral 300,000 U single-dose vitamin D regimen to correct vitamin D deficiency can positively impact outcomes following primary TJA.
Changes in Histological Features, Apoptosis and Necroptosis, and Inflammatory Status in the Livers and Kidneys of Young and Adult Rats
(2024-01-01) HEKİMOĞLU E. R.; EŞREFOĞLU M.; Elibol B.; KIRMIZIKAN S.; HEKİMOĞLU, EMİNE RÜMEYSA; EŞREFOĞLU, MUKADDES; ELİBOL, BİRSEN; KIRMIZIKAN, SEDA
Objective: Aging entails a gradual rise in low-grade inflammation affected by cellular degeneration and death. Inflammaging refers to the chronic, low-grade inflammation that occurs alongside the aging process. This study attempts to evaluate the hepatic and renal histological changes, apoptosis and necroptosis rates, and inflammaging status of 6-week-old and 10-month-old rats. Materials and Methods: This study uses 12 male rats separated into two groups: Young Group (6-week-old rats; n = 6), and Adult Group (10-month-old rats; n = 6). Animals were sacrificed under anesthesia. The rats\" livers and kidneys were removed, and each organ tissue was divided into two parts: one for the microscopic examination (H&E and TUNEL immunohistochemistry) and the other for biochemical determination (tumor necrosis factor-alpha [TNF-α], nuclear factor-kappa beta [NF-κB], interleukin1-Beta [IL-1β], IL6, receptor-interacting serine/threonine protein kinase [RIP], and RIP3). Results: The histological features of the livers and kidneys of the 6-week-old rats were consistent with healthy mammalian organ features, while some histological changes were detected in sections of the 10-month-old rats. The apoptosis rate indicated by TUNEL immunohistochemistry was seen to have increased in the 10-month-old rats, while the necroptosis rate indicated by RIP3 Western-blotting analysis was conversely determined to have decreased. Significant increases in TNF-α and NF-κB levels were consistent with the increased apoptosis rate in the 10-month-old rats compared to the 6-week-old rats. Conclusion: One of the striking results of this study is that the degenerative changes related to aging began to be seen even in 10-month-old rats. The researchers used healthy rats of this age as control subjects as well as to create experimental models.
Presence of Auditory Pathway Abnormalities in Children With Neurofibromatosis Type 1 With Brainstem Focal Areas of Abnormal Signal Intensity: Diffusion Tensor Imaging Features
(2024-06-10) Cesme D. H.; Atasoy B.; Alkan G.; Peker A. A.; Yilmaz T. F.; Yurtsever I.; Iscan A.; Alkan A.; ÇEŞME, DİLEK HACER; ATASOY, BAHAR; PEKER, ABDUSSELİM ADİL; YILMAZ, TEMEL FATİH; YURTSEVER, İSMAİL; İŞCAN, AKIN; ALKAN, ALPAY
Background: To investigate whether there is a difference in mean diffusivity (MD) and fractional anisotropy (FA) values in the auditory pathways of neurofibromatosis type 1 patients with and without focal areas of abnormal signal intensity (FASI) compared to healthy controls by using diffusion tensor imaging (DTI). Methods: Patients were classified as group 1 with focal areas of abnormal signal intensity in the brainstem, group 2 without focal areas of abnormal signal intensity, and healthy control group 3 according to the MRI findings. Mean diffusivity and fractional anisotropy values of lateral lemniscus, inferior colliculus, corpus geniculatum mediale, Heschl gyrus, and brainstem were compared between groups. The correlation between mean diffusivity and fractional anisotropy values of auditory pathways and age was investigated. Results: There was a significant difference between group 1 and group 2 in terms of mean diffusivity and fractional anisotropy values at lateral lemniscus, inferior colliculus, corpus geniculatum mediale, and Heschl gyrus. Increased mean diffusivity and decreased fractional anisotropy values at brainstem were found in group 1. There was a significant difference between group 1 and group 3 in terms of mean diffusivity values at all auditory pathways. Fractional anisotropy values obtained from lateral lemniscus, inferior colliculus, and Heschl gyrus decreased in group 1 compared with group 3. There was a negative correlation between mean diffusivity values and positive correlation between fractional anisotropy values at lateral lemniscus, inferior colliculus, Heschl gyrus, and age. Conclusions: Our diffusion tensor imaging findings show that the neuronal integrity of the auditory pathways is affected in neurofibromatosis type 1 patients with brainstem focal areas of abnormal signal intensity. We think that the disappearance of brainstem focal areas of abnormal signal intensity associated with myelin repair and the regression of diffusion tensor imaging changes in the auditory pathways occur simultaneously with advancing age in patients with neurofibromatosis type 1.
Predictive significance of intraprostatic volumetric parameters derived from early and standard time ⁶⁸Ga-PSMA PET/CT images in newly diagnosed prostate cancer patients
(2024-07-01) ERDOĞAN E. B.; TEKÇE E.; KOCA S.; Aslan N.; TOLUK Ö.; AYDIN M.; ERDOĞAN, EZGİ BAŞAK; TEKÇE, ERTUĞRUL; KOCA, SERHAT; TOLUK, ÖZLEM; AYDIN, MEHMET
Objective: To investigate the relationship between intraprostatic 68Ga-prostate-specific membrane antigen (PSMA) uptake values and volumetric parameters derived from early pelvic and standard-time whole-body 68Ga-PSMA PET/computed tomography (CT) images in untreated prostate cancer (PCa) patients, and to assess the predictive significance of these data in relation to disease prognosis, comparing them with the Gleason score, clinical risk classification and the presence of metastatic disease detected in 68Ga-PSMA PET/CT imaging. Methods: Eighty-one newly diagnosed PCa patients underwent early phase pelvic imaging at the 5th minute and standard time whole-body imaging at the 60th minute. Various threshold values were used in intraprostatic delineations to compute maximum standardized uptake value (SUVmax), mean standardized uptake value (SUVmean), intraprostatic PSMA tumor volume and intraprostatic total lesion PSMA uptake. Correlations between early and standard time measurements, as well as changes in SUV parameters over time, were examined. The association of these values with Gleason score, clinical risk status (National Comprehensive Cancer Network), and metastatic disease was explored. Results: SUVmax measurements from both early and standard time images distinguished all three groups (clinical risk scores, Gleason score and metastatic group), with standard imaging demonstrating statistical superiority in receiver operating characteristic analyses. Strong correlations were observed between early and standard-time PET parameters. Changes in intraprostatic SUVmax and SUVmean values over time did not exhibit predictive value. Conclusion: Although intraprostatic PSMA PET parameters generally aligned at both early and standard times, parameters obtained from standard time images showed more robust correlations with clinical risk scores, Gleason score and metastasis status in newly diagnosed, untreated PCa patients.
Association between self-reported visual symptoms (suggesting cataract) and self-reported fall-related injury among adults aged ≥ 65 years from five low- and middle-income countries.
(2024-06-15) Smith L.; López Sánchez G. F.; Veronese N.; Soysal P.; Tully M. A.; Gorely T.; Allen P. M.; Rahmati M.; Yon D. K.; Ball G.; et al.; SOYSAL, PINAR
Background: Cataracts may increase risk for falls but studies on this topic from low- and middle-income countries (LMICs) are scarce. Therefore, we examined the cross-sectional association between self-reported visual symptoms (suggesting cataract) and self-reported injurious falls in nationally representative samples of adults aged ≥ 65 years from five LMICs (China, Ghana, India, Mexico, and Russia). Methods: Data from the WHO Study on global AGEing and adult health (SAGE) were analysed. Self-reported information on past 12-month fall-related injury and cataract based on symptoms were collected. Multivariable logistic regression and meta-analyses were conducted to assess associations. Results: Data on 13,101 people aged ≥ 65 years were analysed [mean (SD) age 72.5 (11.3) years; 45.2% males]. The overall prevalence of self-reported fall-related injury and visual symptoms (suggesting cataract) were 4.9% and 29.4%, respectively. There was a positive association between self-reported visual symptoms (suggesting cataract) and fall-related injury (i.e., OR > 1) in all five countries but statistical significance was reached in three: China (OR = 1.60; 95% CI = 1.08-2.35), India (OR = 1.96; 95% CI = 1.15-3.35), and Russia (OR = 3.58; 95% CI = 2.06-6.24). The pooled OR including all five countries based on a meta-analysis was OR = 1.88 (95% CI = 1.32-2.68). Conclusions: Self-reported visual symptoms (suggesting cataract) were associated with higher odds for self-reported injurious falls among older adults in LMICs. Expanding availability of cataract surgery in LMICs may also have the additional benefit of reducing falls among older people.
AGE EFFECT ON TYMPANOMETRIC EVALUATION RESULTS
(2024-05-01) Gedik Toker Ö.; Kuru E.; Özbay B.; Akbıyık A. R.; Aksakal E. İ.; Bal N.; GEDİK, ÖZGE
Heparin Enjeksiyonu Uygulamasına Yönelik YouTube™ Videolarının Bilgi, Güvenilirlik ve Kalite Analizi
(2024-04-01) Demircan B.; Kiyak Y.; Kaya H.; DEMİRCAN, BURCU; KIYAK, YASEMIN
Amaç: YouTube hastalar ve sağlık profesyonelleri tarafından bilgiye erişim sitesi olmakla birlikte sağlık okuryazarlığı için eğitim aracı rolü gören bir video paylaşım sitesidir. Araştırma, YouTube video platformunda yer alan heparin enjeksiyonu uygulamasına yönelik videoların bilgi içeriği, kalite ve güvenilirliğini belirlemek amacıyla tanımlayıcı tipte gerçekleştirildi. Yöntemler: YouTube’da 01.02.2024 tarihinde \"deri altına enjeksiyon, \"heparin enjeksiyonu\" ve \"kan sulandırıcı iğne\" anahtar kelimeleri ile yapılan arama sonucunda ulaşılan 31 Türkçe video bağımsız iki gözlemci tarafından Faydalılık, Tüketici Sağlığı Kalite Kriterleri (DISCERN), Küresel Kalite Skalası (GQS), video süresi, görüntüleme sayısı, beğeni sayısı, yorum sayısı, YouTube’daki süresi açısından analiz edildi. Bulgular: Videoların %80,6’sının faydalı, %58,1’inin orta kalitede, %38,7’sinin orta derecede eğitim bilgisine sahip olduğu görüldü. Hemşireler tarafından yüklenen videoların USEFULNESS puanlarının hekimler ve bireysel olarak yüklenen videolara göre (P=,048), Sağlık kuruluşları ve hemşireler tarafından yüklenen videoların sırasıyla DISCERN (P=,025; P=,030) ve GQS puanlarının (P=,035; P=,005) bireysel olarak yüklenen videolara göre anlamlı derecede yüksek olduğu sonucuna ulaşıldı. Video süresi ile DISCERN (r=,499; P=,004), görüntüleme sayısı ile USEFULNESS (r=,370; P=,040) ve GQS (r=,499; P=,004), beğeni sayısı ile GQS (r=,483; P=,006) arasında pozitif yönde orta düzeyde bir ilişki bulundu. Sonuç: YouTube’da yer alan heparin enjeksiyonu videolarının orta kalitede olduğu ve hemşireler tarafından yüklenen videoların daha faydalı olduğu bulundu. Anahtar Kelimeler: Heparin enjeksiyonu, kan sulandırıcı iğne, sosyal medya, subkutan heparin enjeksiyonu, YouTube.
Olive Leaf Extract Downregulates the Protein Expression of Key SARS-CoV-2 Entry Enzyme ACE-2, TMPRSS2, and Furin.
(2024-06-04) Kocyigit A.; Kanimdan E.; Yenigun V. B.; Ozman Z.; Balıbey F. B.; Durmuş E.; Yasar O.; KOÇYİĞİT, ABDÜRRAHİM; YAŞAR, ÖZNUR; KANIMDAN, EBRU; YENİGÜN, VILDAN BETÜL; ÖZMAN, ZEYNEP; BABALI BALIBEY, FATMANUR
Severe acute respiratory syndrome coronavirus 2 poses ongoing global health challenges due to its propensity for mutations, which can undermine vaccine efficacy. With no definitive treatment available, urgent research into affordable and biocompatible therapeutic agents is extremely urgent. Angiotensin converting enzyme-2 (ACEII), transmembrane protease serine subtype 2 (TMPRSS2), and Furin enzymes, which allow the virus to enter cells, are particularly important as potential drug targets among scientists. Olive leaf extract (OLE) has garnered attention for its potential against COVID-19, yet its mechanism remains understudied. In this study, we aimed to investigate the effects of OLE on ACEII, TMPRSS2, and Furin protein expressions by cell culture study. Total phenol, flavonoid content, and antioxidant capacity were measured by photometric methods, and oleuropein levels were measured by liquid LC-HR-MS. Cell viability was analyzed by ATP levels using a luminometric method. ACEII, TMPRSS2, and Furin expressions were analyzed by the Western Blotting method. ACEII, TMPRSS2, and Furin protein expression levels were significantly lower in dose dependent manner and the highest inhibition was seen at 100 ug/ml OLE. The results showed that OLE may be a promising treatment candidate for COVID-19 disease. However, further studies need to be conducted in cells co-infected with the virus.
Evaluating the accuracy and readability of ChatGPT in providing parental guidance for adenoidectomy, tonsillectomy, and ventilation tube insertion surgery
(2024-06-01) POLAT E.; Polat Y. B.; ŞENTÜRK E.; DOĞAN R.; YENİGÜN A.; Tugrul S.; EREN S. B.; AKSOY F.; ÖZTURAN O.; POLAT, EMRE; ŞENTÜRK, EROL; DOĞAN, REMZI; YENİGÜN, ALPER; TUĞRUL, SELAHATTİN; EREN, SABRİ BAKİ; AKSOY, FADLULLAH; ÖZTURAN, ORHAN
Objectives: This study examined the potential of ChatGPT as an accurate and readable source of information for parents seeking guidance on adenoidectomy, tonsillectomy, and ventilation tube insertion surgeries (ATVtis). Methods: ChatGPT was tasked with identifying the top 15 most frequently asked questions by parents on internet search engines for each of the three specific surgical procedures. We removed repeated questions from the initial set of 45. Subsequently, we asked ChatGPT to generate answers to the remaining 33 questions. Seven highly experienced otolaryngologists individually assessed the accuracy of the responses using a four-level grading scale, from completely incorrect to comprehensive. The readability of responses was determined using the Flesch Reading Ease (FRE) and Flesch-Kincaid Grade Level (FKGL) scores. The questions were categorized into four groups: Diagnosis and Preparation Process, Surgical Information, Risks and Complications, and Postoperative Process. Responses were then compared based on accuracy grade, FRE, and FKGL scores. Results: Seven evaluators each assessed 33 AI-generated responses, providing a total of 231 evaluations. Among the evaluated responses, 167 (72.3 %) were classified as ‘comprehensive.’ Sixty-two responses (26.8 %) were categorized as ‘correct but inadequate,’ and two responses (0.9 %) were assessed as ‘some correct, some incorrect.’ None of the responses were adjudged ‘completely incorrect’ by any assessors. The average FRE and FGKL scores were 57.15(±10.73) and 9.95(±1.91), respectively. Upon analyzing the responses from ChatGPT, 3 (9.1 %) were at or below the sixth-grade reading level recommended by the American Medical Association (AMA). No significant differences were found between the groups regarding readability and accuracy scores (p > 0.05). Conclusions: ChatGPT can provide accurate answers to questions on various topics related to ATVtis. However, ChatGPT\"s answers may be too complex for some readers, as they are generally written at a high school level. This is above the sixth-grade reading level recommended for patient information by the AMA. According to our study, more than three-quarters of the AI-generated responses were at or above the 10th-grade reading level, raising concerns about the ChatGPT text\"s readability.
Synthesis of pyrrole-heterocyclic derivatives as anti-Alzheimer and antidiabetic candidates: An in vitro-in silico study
(2024-06-01) Petrova O. V.; Tomilin D. N.; Şenol H.; Belyaeva K. V.; Nikitina L. P.; Oparina L. A.; Sobenina L. N.; Trofimov B. A.; Sadeghian N.; Taslimi P.; et al.; ŞENOL, HALIL

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