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Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

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Demirci, Mehmet
Saribas, Suat
Ozer, Nigar
TOPRAK, Sezer
CAGLAR, Emel
ORTAKOYLU, Gonenc
Yuksel, PELİN
Ayaz, Gulsel
Tokman, Hrisi B.
Uysal, Omer

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Background: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.

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Demirci M., Saribas S., Ozer N., TOPRAK S., CAGLAR E., ORTAKOYLU G., Yuksel P., Ayaz G., Tokman H. B. , Uysal O., et al., -Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection-, Journal of Infection and Public Health, cilt.11, ss.662-666, 2018

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