Publication: Treatment of ovarian damage induced by chemotherapeutic drugs in female rats with G-CSF and platelet-rich plasma(PRP): an immunohistochemical study correlation with novel marker INSL-3
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Date
2024-01-01
Authors
ÇETİN Ç.
Okten S. B.
TOK O. E.
Ozcan P.
GÖKMEN KARASU A. F.
Tanoglu F. B.
Taha H. S.
ATEŞ S.
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Abstract
Objective: To assess the impacts of Platelet-Rich Plasma(PRP) and Granulocyte Colony-Stimulating Factor(G-CSF) on a rat model with induced ovarian follicular damage caused by cyclophosphamide(Cy). Materials and Methods: Forty-two Sprague–Dawley rats were randomly allocated into seven distinct groups as; Group 1(control): NaCl intraperitoneal (IP) injection was administered on days D1, D7, and D14. Group 2(Cy):Cy IP injection on D1 + NaCl IP injection on D7 and D14 were administered. Group 3(PRP): PRP IP injection on D1,D7 and D14 were administered. Group 4(Cy + PRP):Cy IP injection on D1 and PRP IP injection on D1, D7 and D14 were administered. Group 5(G-CSF): G-CSF IP injection on D1, D7 and D14 were administered. Group 6(Cy + G-CSF):Cy IP injection on D1+ G-CSF IP injection on D1, D7 and D14 were administered. Group 7(Cy + PRP + G-CSF):Cy IP injection on D1+ PRP IP injection on D1,D7 and D14+ G-CSF IP injection on D1,D7 and D14 were administered. Follicular number, histological scores of AMH and INSL3 stained follicles at different stages of follicular development, and serum Anti-Müllerian hormone(AMH) were evaluated. Results: The primary, secondary, and antral follicle intensity scores for AMH-positive staining were most prominent in Groups 3 and 5. There was no significant difference between groups 4, 6 and 7 compared to group 1 in terms of follicule counts and AMH staining. The intensity scores of AMH-positive staining follicles were notably reduced in group 2 compared to groups 4, 6, and 7, with a significant difference (p <.01). Among the groups, group 2 exhibited the least intense antral follicle staining for INSL3, displaying a significant difference(p <.01) compared to the remaining groups. Conclusions: Autologous PRP and G-CSF might protect ovarian function in the face of ovarian damage caused by Cy-induced effects.
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Keywords
Tıp, Dahili Tıp Bilimleri, İç Hastalıkları, Endokrinoloji ve Metabolizma Hastalıkları, Cerrahi Tıp Bilimleri, Kadın Hastalıkları ve Doğum, Sağlık Bilimleri, Medicine, Internal Medicine Sciences, Internal Diseases, Endocrinology and Metabolic Diseases, Surgery Medicine Sciences, Obstetrics and Gynecology, Health Sciences, Klinik Tıp (MED), Klinik Tıp, ENDOKRİNOLOJİ VE METABOLİZMA, KADIN HASTALIKLARI & DOĞUM, Clinical Medicine (MED), CLINICAL MEDICINE, ENDOCRINOLOGY & METABOLISM, OBSTETRICS & GYNECOLOGY, Endokrinoloji, Diyabet ve Metabolizma, Endokrinoloji, Yaşam Bilimleri, Doğum ve Jinekoloji, Endocrinology, Diabetes and Metabolism, Endocrinology, Life Sciences, Follicle, G-csf, INSL3 ovary, ovarian failure, platelet-rich plasma
Citation
ÇETİN Ç., Okten S. B., TOK O. E., Ozcan P., GÖKMEN KARASU A. F., Tanoglu F. B., Taha H. S., ATEŞ S., "Treatment of ovarian damage induced by chemotherapeutic drugs in female rats with G-CSF and platelet-rich plasma(PRP): an immunohistochemical study correlation with novel marker INSL-3", Gynecological Endocrinology, cilt.40, sa.1, 2024