Person: ÇETİN, ÇAĞLAR
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Publication Treatment of ovarian damage induced by chemotherapeutic drugs in female rats with G-CSF and platelet-rich plasma(PRP): an immunohistochemical study correlation with novel marker INSL-3(2024-01-01) ÇETİN Ç.; Okten S. B.; TOK O. E.; Ozcan P.; GÖKMEN KARASU A. F.; Tanoglu F. B.; Taha H. S.; ATEŞ S.; ÇETİN, ÇAĞLAR; ÖZCAN, PINAR; GÖKMEN KARASU, AYŞE FİLİZ; TANOĞLU, FATMA BAŞAK; ATEŞ, SEDAObjective: To assess the impacts of Platelet-Rich Plasma(PRP) and Granulocyte Colony-Stimulating Factor(G-CSF) on a rat model with induced ovarian follicular damage caused by cyclophosphamide(Cy). Materials and Methods: Forty-two Sprague–Dawley rats were randomly allocated into seven distinct groups as; Group 1(control): NaCl intraperitoneal (IP) injection was administered on days D1, D7, and D14. Group 2(Cy):Cy IP injection on D1 + NaCl IP injection on D7 and D14 were administered. Group 3(PRP): PRP IP injection on D1,D7 and D14 were administered. Group 4(Cy + PRP):Cy IP injection on D1 and PRP IP injection on D1, D7 and D14 were administered. Group 5(G-CSF): G-CSF IP injection on D1, D7 and D14 were administered. Group 6(Cy + G-CSF):Cy IP injection on D1+ G-CSF IP injection on D1, D7 and D14 were administered. Group 7(Cy + PRP + G-CSF):Cy IP injection on D1+ PRP IP injection on D1,D7 and D14+ G-CSF IP injection on D1,D7 and D14 were administered. Follicular number, histological scores of AMH and INSL3 stained follicles at different stages of follicular development, and serum Anti-Müllerian hormone(AMH) were evaluated. Results: The primary, secondary, and antral follicle intensity scores for AMH-positive staining were most prominent in Groups 3 and 5. There was no significant difference between groups 4, 6 and 7 compared to group 1 in terms of follicule counts and AMH staining. The intensity scores of AMH-positive staining follicles were notably reduced in group 2 compared to groups 4, 6, and 7, with a significant difference (p <.01). Among the groups, group 2 exhibited the least intense antral follicle staining for INSL3, displaying a significant difference(p <.01) compared to the remaining groups. Conclusions: Autologous PRP and G-CSF might protect ovarian function in the face of ovarian damage caused by Cy-induced effects.Publication The Role of the Urine Dipstick Test in the Detection of Abnormal Proteinuria Using Different Cut-off Levels in Hypertensive Pregnancies Hipertansif Gebeliklerde Farklı Kesim Değerleri Kullanılarak Anormal Proteinüri Saptanmasında Spot İdrar Protein Ölçümünün Rolü(2022-01-01) Takmaz T.; Gorchiyeva I.; Arici Halici B. N.; TOPRAK A.; ÇETİN Ç.; KÜTÜK M. S.; TAKMAZ, TAHA; ARICI HALICI, BELFİN NUR; TOPRAK, ALİ; ÇETİN, ÇAĞLAR; KÜTÜK, MEHMET SERDAR© 2022, Duzce University Medical School. All rights reserved.Aim: The aim of this study was to determine the diagnostic accuracy of different urine dipstick protein threshold levels in predicting the presence of abnormal proteinuria in pregnant women with hypertension. Material and Methods: A total of 326 singleton pregnant women who underwent 501 urine protein tests and who had suspected preeclampsia were included in this retrospective study. Patient data was taken including medical and obstetric history. The results of dipstick urinalysis and concurrent 24-hour urine protein excretion measurements were compared to determine the accuracy of urinalysis. Results: A dipstick result of 1+ was found to be the best cut-off to predict 500 mg of protein excretion per day, with sensitivity and specificity of 62.09% and 88.97%, respectively. A 2+ proteinuria dipstick cut-off had high specificity and positive predictive value (PPV) (99.05% and 98.84%, respectively) for the prediction of 300 mg of protein excretion per day; this cut-off had low sensitivity (21.46%). A cut-off of 1+ also provided satisfactory specificity and PPV (91.43% and 94.48%, respectively) for the detection of 300 mg of protein excretion per day, but sensitivity was compromised (38.89%). Among 301 patients with negative dipstick results, 212 had a 24-hour urine protein extraction greater than 300 mg, with a false negative rate of 70.43%. Conclusion: The results suggest that the urine protein dipstick measurement has limited quantitative ability for the prediction of abnormal proteinuria. Additionally, the use of 500 mg 24-hours protein excretion as a cut-off value for abnormal proteinuria may provide useful data.Publication Cannabidiol as a potential novel treatment for endometriosis by its anti-inflammatory, antioxidative and antiangiogenic effects in an experimental rat model(2023-01-01) Okten S. B.; ÇETİN Ç.; Tok O. E.; Guler E. M.; Taha S. H.; ÖZCAN P.; Ficicioglu C.; ÇETİN, ÇAĞLAR; ÖZCAN, PINARResearch question: Can cannabidiol (CBD) be used in the treatment of endometriosis for its anti-inflammatory, antioxidative and antiangiogenic effects? Design: Endometrial implants were surgically induced in 36 female Wistar albino rats. After confirmation of endometriotic foci, the rats were randomized into four groups. In the leuprolide acetate group, rats were given a single 1 mg/kg s.c. leuprolide acetate injection. The other groups were 5 mg/kg CBD (CBD5), saline solution and 20 mg/kg CBD (CBD20); daily i.p. injections were administered for 7 days. After 21 days, the rats were euthanised, and total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) measurements in blood and peritoneal fluid samples, and immunohistochemical staining for TNF-α, IL-6 and vascular endothelial growth factor (VEGF) of endometriotic tissues were evaluated. Results: Significant reductions in the endometriotic implant surface area (P = 0.0213), serum TOS (P = 0.0491), OSI (P = 0.0056), IL-6 (P = 0.0236), TNF-α (P = 0.0083) and peritoneal fluid OSI (P = 0.0401), IL-6 (P = 0.0205) and TNF-α (P = 0.0045) concentrations were observed in the CBD5 group when compared with the saline solution group. Compared with the saline solution group, increased TAS concentrations in serum (P = 0.0012) and peritoneal fluid (P = 0.0145) were found in the CBD5 group. The CBD5 and leuprolide acetate groups were similar regarding inflammatory and oxidative stress parameters of serum and peritoneal fluid samples. The CBD5 group showed significantly lower mean intensity in both surface epithelium and stromal cells for VEGF (both P = 0.002) and only in surface epithelium cells for IL-6 (P = 0.0108), when compared with the leuprolide acetate group. Conclusion: Due to its anti-inflammatory, antioxidative and antiangiogenic effects, CBD might be a therapeutic agent candidate for endometriosis.Publication Evaluation of chronotype and sleep quality in infertile population and comparison with fertile population: a cross-sectional study(2023-12-01) Özçelik C.; VARLI B.; Gökçe A.; Takmaz T.; ÇETİN Ç.; ÖZCAN P.; TAKMAZ, TAHA; ÇETİN, ÇAĞLAR; ÖZCAN, PINARPURPOSE: Infertility is a stressful condition for couples and can affect patients\" circadian rhythm and sleep quality. The goal of this study is to assess differences in chronotype and sleep quality between infertile and fertile people. METHODS: A cross-sectional study was conducted. The infertile patient population consisted study group. Primiparous patients without any known gynecological disease who presented for routine cervical cancer screening follow-up were included in the control group. The Turkish version of the Morningness-Eveningness Questionnaire (MEQ) and Pittsburg Sleep Quality Index (PSQI) scores were evaluated between groups. RESULTS: A total of 227 patients were assessed. There were 110 patients in the study (infertile) group and 117 patients in the control (fertile) group. The evening chronotype proportion (23.6 vs. 0.9%, p < 0.001) was higher in the infertile group. The median of MEQ score was significantly higher in the fertile patients (50, IQR = 43 - 55 vs. 56, IQR = 51 - 59; p < 0.001), and the median of PSQI score was significantly higher in the infertile patients (5, IQR = 4 - 6, vs. 4, IQR = 3 - 5; p < 0.001). CONCLUSIONS: In this study, we found significantly worse sleep quality, and more evening chronotype in the patients with infertility.Publication Excision of subcutaneous endometriosis lesions in obese patients by marking them with methylene blue with ultrasound guidance: a novel technique(2023-01-01) ÇETİN Ç.; KÜTÜK M. S.; Tanoğlu F. B.; ATEŞ S.; ÖZCAN P.; BAKAR R. Z.; ÇETİN, ÇAĞLAR; KÜTÜK, MEHMET SERDAR; TANOĞLU, FATMA BAŞAK; ATEŞ, SEDA; ÖZCAN, PINAR; BAKAR, RABIA ZEHRAPublication Effect of maternal smoking during pregnancy on fetal circulation and growth(2023-06-25) Kütük M. S.; Bakar R. Z.; Toluk Ö.; Çetin Ç.; Yozgat C. Y.; Doygun Ö.; KÜTÜK, MEHMET SERDAR; BAKAR, RABIA ZEHRA; TOLUK, ÖZLEM; ÇETİN, ÇAĞLARObjective To understand the pathogenic mechanism of smoking on abnormal fetal growth by assessing the umbilical, and middle cerebral pulsatility index (MCA- PI, and UmA-PI, respectively) cerebroplacental ratio (CPR) and middle MCA peak systolic velocity (MCA-Vmax). Methods A hundred and eighty-one pregnant women were included in the study, 118 of whom were ever smoker and 62 were smoker. Smoking status of the cases were based on the participants statements and confirmed with breath CO test. Patients were undergo Doppler examination according to thecurrent guidelines at 24, 28, 32 and 36 weeks and the results were expressed as z- scores. UmA- PI, MCA-PI, CPR (MCI-PI/UmA-PI), MCA- Vmax were compared between control and smoking groups. As a subgroup analysis, effect of heavy smoking (>10/day) on fetal growth and Doppler indexes were also compared. Results Body mass index (BMI), gravida, rate of gestational hypertension and neonatal intensive care admission is significantly higher in smoking group. Birthweight and gestational age at birth was significantly higher in control group [3355 (1960.0-4660.0) vs 3162.5(550.0-4190.0), p=0.003, and274.0(250.0-294.0) vs. 271(175.0-288.0), p=0.015, respectively]. When controlled for confounding factors, no significant difference was detectedbetween smokers and control groups with regard to UmA-PI, MCA-PI, MCA- V Max and CPR, at 24 , 28 , 32 , 36 weeks. EFW at all gestational ages were significantly lower in smoker group (p=0.033). At subgroup analysis, no significant difference was detected between control and heavy smokers in terms of EFW and all Doppler parameters. Conclusion Smoking during pregnancy is associated decreased fetal growth and increased abnormal neonatal outcome. However, it has no demonstrable effect on any of the Doppler indexes and velocities at any given gestational ages. Abnormal effect of smoking on fetal growth seems not to operate throughimpaired uteroplacental circulationPublication Comparison of Patients with Trucut Biopsy , Acid Cytology with Final Pathology Results from Patients Operated with Prediagnose of Ovarian Cancer(2022-10-30) Tanoğlu F. B.; Çetin Ç.; Pasin Ö.; Kıran G.; ÇETİN, ÇAĞLAR; PASİN, ÖZGE; KIRAN, GÜRKANPublication The importance of serum progesterone concentration at embryo transfer day and effect of rescue additional progesterone during programmed artificial frozen embryo transfer cycles(2022-01-01T00:00:00Z) ÖZCAN, PINAR; ÇETİN, ÇAĞLAR; Okten, Berkem; TANOĞLU, FATMA BAŞAK; Taha, Havva Sevde; Pasin, Ozge; Ficicioglu, Cem; ÖZCAN, PINAR; ÇETİN, ÇAĞLAR; TANOĞLU, FATMA BAŞAK; PASİN, ÖZGE© 2022 Reproductive Healthcare Ltd.Research question: What is the efficacy and possible positive effect of additional rescue subcutaneous (s.c.) progesterone therapy for restoration of progesterone concentration on embryo transfer day and pregnancy rates during programmed artificial frozen embryo transfer (FET) cycles with vaginal progesterone tablet plus i.m. progesterone? Design: Multicentre prospective cohort study (NCT04769401) including a total of 238 programmed artificial FET between February 2021 and September 2021. Patients were divided into ≥10 ng/ml and <10 ng/ml according to serum progesterone concentrations on embryo transfer day; 25 mg of s.c. progesterone was added to patients with <10 ng/ml; blood samples were taken 2 days later. The primary outcome was the ongoing pregnancy rate. Results: The proportion of patients having ≥10 ng/ml serum progesterone concentrations on embryo transfer day was 70.7%. There was no statistically significant difference with regard to cumulative pregnancy rate, ongoing pregnancy rate and miscarriage between the groups (55.4% versus 61.5%, P = 0.4; 78.2% versus 72.5% per pregnancy, P = 0.5; 21.8% versus 27.5%, P = 0.5, respectively, ≥10 ng/ml and <10 ng/ml). Eighty-three per cent of patients with low serum progesterone concentrations on embryo transfer day reached an adequate progesterone concentration with rescue s.c. progesterone treatment; 90% of pregnancy rates in patients with serum progesterone concentrations <10 ng/ml on embryo transfer day were in patients who reached adequate serum progesterone concentrations with daily rescue s.c. progesterone treatment. Conclusions: The measurement of serum progesterone concentrations on embryo transfer day may create the opportunity for rescue progesterone administration on that day for patients who fail to reach adequate serum progesterone concentrations, achieving similar pregnancy rates without cancellation of the cycle.Publication The effect of serum progesterone level on ET Day and the efficacy of rescue progesterone treatment on ongoing pregnancy rates with artificial FET cycles(2022-07-01T00:00:00Z) ÖZCAN, PINAR; ÇETİN, ÇAĞLAR; Okten, B.; Tanoglu, F. B.; İŞLEK, HAVVA SEVDE; PASİN, ÖZGE; Ficicioglu, C.; ÖZCAN, PINAR; ÇETİN, ÇAĞLAR; İŞLEK, HAVVA SEVDE; PASİN, ÖZGEPublication The Effects of Maternal Smoking on Thyroid Function: Findings from Routine First-Trimester Sonographic Anomaly Screening(2023-01-01) BAKAR R. Z.; ÇETİN Ç.; Yozgat C. Y.; KÜTÜK M. S.; BAKAR, RABIA ZEHRA; ÇETİN, ÇAĞLAR; KÜTÜK, MEHMET SERDARAim This study aimed to assess the effect of tobacco exposure on maternal thyroid function and investigate its relationship to subclinical hypothyroidism in pregnant women during the first trimester. Subjects and Method A comparison of maternal thyroid function was made on 45 smokers, who composed the study group, and 72 non-smokers, pregnant women, who constituted the control group. After determining smokers by questionnaire, carbon monoxide (CO) levels in the expiratory air of the participants in both groups were measured and recorded, and the smokers\" exposure was objectively confirmed. Results Smoking and non-smoking pregnant women were similar regarding body mass index (BMI). While the TSH and fT4 levels were respectively 1.48 mlU/L and 11.43 pmol/L in pregnant women who smoked, that ratio changed to 1.72 mlU/L and 11.17 pmol/L in the non-smokers\" group. But the differences between the groups were not statistically significant (p=0.239, p=0.179). Even though the rate of subclinical hypothyroidism was 8.9% in the smoking group, it was approximately 19.4% in the non-smoker group; the difference was not statistically significant (p=0.187). Conclusion This study proved that there is no statistically significant difference between maternal serum TSH and fT4 levels and the rate of subclinical hypothyroidism in smokers during pregnancy in the first trimester.