Publication:
1,25-Dihydroxyvitamin D-3 stimulates odontoblastic differentiation of human dental pulp-stem cells in vitro

No Thumbnail Available
Date
2017-01-01T00:00:00Z
Authors
MUCUK, GÖKŞEN
SEPET, ELİF
ERGÜVEN, MİNE
EKMEKÇİ, ÖZLEM
BİLİR, AYHAN
Journal Title
Journal ISSN
Volume Title
Publisher
Research Projects
Organizational Units
Journal Issue

Metrics

Search on Google Scholar

Abstract
Background: 1,25-Dihydroxyvitamin D-3 (1,25-OH D-3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). Aim: This study aimed to understand the influence of various concentrations of 1,25-OH D-3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. Materials and Methods: hDPSCs were obtained from the impacted third molar teeth. Monolayer cultured cells were incubated with a differentiation medium containing different concentrations of 1,25-OH D-3 (0.001, 0.01, and 0.1 mu M). All groups were evaluated by S-phase rate [immunohistochemical (IHC) bromodeoxyuridine (BrdU) staining], STRO-1 and dentin sialoprotein (DSP)+ levels (IHC), and alkaline phosphatase (ALP, enzyme-linked immunosorbent assay (ELISA)) levels. Results: The number of cells that entered the S-phase was determined to be the highest and lowest in the control and 0.001 mu M 1,25-OH D-3 groups, respectively. The 0.1 mu M vitamin D-3 group had the highest increase in DSP+ levels. The highest Stro-1 levels were detected in the control and 0.1 mu M 1,25-OH D-3 groups, respectively. The 0.1 mu M 1,25-OH D-3 induced a mild increase in ALP activity. Conclusions: This study demonstrated that 1,25-OH D-3 stimulated odontoblastic differentiation of hDPSCs in vitro in a dose-dependent manner. The high DSP + cell number and a mild increase in ALP activity suggest that DPSCs treated with 0.1 M 1,25-OH D-3 are in the later stage of odontoblastic differentiation. The results confirm that 1,25-OH D-3-added cocktail medium provides a sufficient microenvironment for the odontoblastic differentiation of hDPSCs in vitro.
Description
Keywords
Citation
MUCUK G., SEPET E., ERGÜVEN M., EKMEKÇİ Ö., BİLİR A., -1,25-Dihydroxyvitamin D-3 stimulates odontoblastic differentiation of human dental pulp-stem cells in vitro-, CONNECTIVE TISSUE RESEARCH, cilt.58, no.6, ss.531-541, 2017
Collections
Page Views

3

File Downloads

0

Sustainable Development Goals