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MUCUK, GÖKŞEN

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GÖKŞEN
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MUCUK
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    1,25-Dihydroxyvitamin D-3 stimulates odontoblastic differentiation of human dental pulp-stem cells in vitro
    (2017-01-01T00:00:00Z) MUCUK, GÖKŞEN; SEPET, ELİF; ERGÜVEN, MİNE; EKMEKÇİ, ÖZLEM; BİLİR, AYHAN; MUCUK, GÖKŞEN
    Background: 1,25-Dihydroxyvitamin D-3 (1,25-OH D-3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). Aim: This study aimed to understand the influence of various concentrations of 1,25-OH D-3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. Materials and Methods: hDPSCs were obtained from the impacted third molar teeth. Monolayer cultured cells were incubated with a differentiation medium containing different concentrations of 1,25-OH D-3 (0.001, 0.01, and 0.1 mu M). All groups were evaluated by S-phase rate [immunohistochemical (IHC) bromodeoxyuridine (BrdU) staining], STRO-1 and dentin sialoprotein (DSP)+ levels (IHC), and alkaline phosphatase (ALP, enzyme-linked immunosorbent assay (ELISA)) levels. Results: The number of cells that entered the S-phase was determined to be the highest and lowest in the control and 0.001 mu M 1,25-OH D-3 groups, respectively. The 0.1 mu M vitamin D-3 group had the highest increase in DSP+ levels. The highest Stro-1 levels were detected in the control and 0.1 mu M 1,25-OH D-3 groups, respectively. The 0.1 mu M 1,25-OH D-3 induced a mild increase in ALP activity. Conclusions: This study demonstrated that 1,25-OH D-3 stimulated odontoblastic differentiation of hDPSCs in vitro in a dose-dependent manner. The high DSP + cell number and a mild increase in ALP activity suggest that DPSCs treated with 0.1 M 1,25-OH D-3 are in the later stage of odontoblastic differentiation. The results confirm that 1,25-OH D-3-added cocktail medium provides a sufficient microenvironment for the odontoblastic differentiation of hDPSCs in vitro.
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    Relationship Between Mandibular Incisor Irregularity and Type of Occlusion in Ankyloglossia
    (2015-01-01T00:00:00Z) Sepet, Elif; Yildiz, Cagla; Erdem, Arzu Pinar; Ikikarakayali, GÖKŞEN; Gorken, Feyza Nur; Kuru, Sinem; MUCUK, GÖKŞEN
    Purpose: To investigate the relationship between the lingual frenulum length with mandibular incisor irregularity and type of occlusion in children with ankyloglossia.