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Protective Effects of Silymarin against Cardiac Tissue Injury Caused By a High-dose Administration of Isotretinoin in Mice

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dc.contributor.authorKumas, MELTEM
dc.contributor.authorEsrefoglu, MUKADDES
dc.contributor.authorOzer, Omer Faruk
dc.contributor.institutionauthorKUMAŞ, MELTEM
dc.contributor.institutionauthorEŞREFOĞLU, MUKADDES
dc.contributor.institutionauthorÖZER, ÖMER FARUK
dc.date.accessioned2019-10-05T13:40:03Z
dc.date.available2019-10-05T13:40:03Z
dc.date.issued2016-08-01
dc.description.abstractObjective: We aimed to histopathologically and biochemically investigate the protective effects of silymarin (SLY) against cardiac injury induced by a high-dose isotretinoin (ISR) administration. Methods: Thirty-two male Balb/c mice were divided into four groups: control, ISR, SLY, and co-treated (ISR+SLY) groups. For the histopathological analysis, all sections were stained with hematoxylin eosin and Masson's trichrome. The TUNEL detection kit was used for the detection of cardiac apoptosis. The oxidative stress markers, which included catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), and glutathione reductase (GSH-red), and the glutathione S-transferase (GST) and lipid peroxidation markers, which included erythrocyte malondialdehyde (E-MDA) and plasma malondialdehyde (P-MDA), were biochemically determined. All statistical analyses were conducted with SPSS 18.0 (IBM, New York, USA) and GraphPad Prism Version 6.01 (USA). The Student's t-test was applied to the parametric data for comprising among groups. The P-significance degree was evaluated as 95%. Results: Severe histopathological alterations were observed in cardiac tissue. The ISR group showed hemorrhage, necrosis, edema, and congestion in cardiac tissues. SLY did not improve ISR-induced histopathological changes (p>0.05). The highest number of apoptotic cells was detected in the ISR group. In the ISR+SLY group, SLY treatment reduced apoptosis (p <= 0.05). Antioxidant enzyme activities (CAT, SOD, GSH-Px, GSH-Red, GSH, GST) were induced in ISR+SLY group (p=0.015, p>0.05, p=0.002, p=0.002, p=0,018, p=0.002; respectively). E-MDA (p=0.003) and P-MDA (p=0.005) levels significantly increased in the ISR group and decreased in the SLY group. Conclusion: SLY increased antioxidant enzyme activities, prevented lipid peroxidation, and decreased apoptosis. SLY was found to be an antioxidant against ISR-induced cardiac oxidative damage.
dc.identifier10.1080/13880209.2017.1310906
dc.identifier.citationKumas M., Esrefoglu M., Ozer O. F. , -Protective Effects of Silymarin against Cardiac Tissue Injury Caused By a High-dose Administration of Isotretinoin in Mice-, BEZMIALEM SCIENCE, cilt.4, ss.43-50, 2016
dc.identifier.doi10.14235/bs.2016.682
dc.identifier.trdizin237206
dc.identifier.urihttps://hdl.handle.net/20.500.12645/2676
dc.identifier.wosWOS:000388331900003
dc.language.isoen
dc.subjectIsotretinoin
dc.subjectsilymarin
dc.subjectheart
dc.subjectoxidative stress
dc.subjectapoptosis
dc.subjecthistopathology
dc.titleProtective Effects of Silymarin against Cardiac Tissue Injury Caused By a High-dose Administration of Isotretinoin in Mice
dc.typeArticle
dspace.entity.typePublication
local.article.journalnamePharmaceutical biology
local.avesis.id4174e84f-763f-439f-ba78-808bd8bbcb08
local.avesis.response2546
local.org.facultyTıp Fakültesi
local.publication.isinternational1
relation.isAuthorOfPublication566662cf-8bbb-4744-bfd8-867d0784f978
relation.isAuthorOfPublicationd0c7c343-3b7d-47ef-a2a7-5169234110eb
relation.isAuthorOfPublicationf5267834-a664-4da6-89f3-ae19762e1423
relation.isAuthorOfPublication.latestForDiscoveryd0c7c343-3b7d-47ef-a2a7-5169234110eb
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