Publication: Evaluation of ciliary functions and ciliary beat frequency via cell culture method in patients with primary ciliary dyskinesia
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Date
2022-07-01T00:00:00Z
Authors
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EMİRALİOĞLU ORDUKAYA, NAGEHAN
HEKİMOĞLU, EMİNE RÜMEYSA
Kaya, Bengisu
Bilgic, Elif
ATİLLA, PERGİN
Gunaydin, Onder
Tugcu, Gokcen Dilsa
Polat, Sanem Eryilmaz
Hizal, Mina Gharibzadeh
Yalcin, Ebru
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Abstract
Background: Cell culture increases both diagnostic specificity and sensitivity of primary ciliary dyskinesia (PCD) and the most important reason to use cell culture for definitive diagnosis in PCD is to exclude secondary ciliary defects. Here we aimed to evaluate the cilia functions and cilia ultrastructural abnormalities after ciliogenesis of cell culture in patients with definitive diagnosis of PCD. We also aimed to compare high speed videomicroscopy (HSVM) results of patients before and after ciliogenesis and to compare them with electron microscopy, genetic and immunofluorescence results in patients with positive diagnosis of PCD.
Methods: This study was conducted as a cross-sectional study in patients with PCD. HSVM, transmission electron microscopy (TEM) and immunofluorescence staining results of the nasal biopsy samples taken from patients with the definitive diagnosis of PCD were evaluated and HSVM findings before and after cell culture were described.
Results: Ciliogenesis and regrowth in the cell culture occurred in the nasal biopsy sample of eight patients with PCD. The mean age of the patients was 15.5±4.2 years (8.5-18 years). Mean beat frequency was found to be 7.54±1.01 hz (6.53-9.45 hz) before cell culture, and 7.36±0.86 hz (6.02-7.99 hz) after cell culture in the nasal biopsy of patients. There was no significant difference in the beat frequency of PCD patients before and after cell culture. Ciliary function analysis showed the similar beating pattern before and after cell culture in patients with PCD.
Conclusions: This study showed us that there was no difference between cilia beat frequency and beat pattern before and after cell culture in patients with definitive diagnosis of PCD and repeated HSVM would be a useful diagnostic approach in patients who have no possibility to reach other diagnostic methods.
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Keywords
cell culture, cilia function analysis, high speed videomicroscopy, primary ciliary dyskinesia
Citation
EMİRALİOĞLU ORDUKAYA N., HEKİMOĞLU E. R. , Kaya B., Bilgic E., ATİLLA P., Gunaydin O., Tugcu G. D. , Polat S. E. , Hizal M. G. , Yalcin E., et al., -Evaluation of ciliary functions and ciliary beat frequency via cell culture method in patients with primary ciliary dyskinesia-, TURKISH JOURNAL OF PEDIATRICS, cilt.64, sa.4, ss.612-618, 2022