Design Of A Burn Treatment Ointment Containing Herbal Bioactives

Abstract

In this study, it was aimed to develop a burn treatment ointment consisting of herbal ingredients, which combination and synergistic effects had not been evaluated together in a formula before. In this regard, herbal ingredients were determined according to their cell proliferation, anti-inflammatory, antioxidant and astringent activities in light of a comprehensive literature review, and then eight ingredients were selected and divided into two groups. First group formulations were prepared by St. John\"s wort oil (Hypericum perforatum L.), bitter melon seed olive oil macerate (Momordica charantia L.), calendula olive oil macerate (Calendula officialis L.), horse chestnut fruit olive oil macerate (Aesculus hippocastanum L.) and second group formulations were prepared by Golden herb flower olive oil macerate (Helichrysum italicum Roth G.), chamomile flower olive oil macerate (Matricaria recutita L.), fenugreek seed oil (Trigonella foenum graecum L.) and grape seed oil (Vitis vinifera L.) as phytoactive ingredients in various amounts, and white beeswax (Cera alba) and vaseline mixture was used in both groups as ointment bases in various amounts to perform optimization of ointment formulations. At the end of preliminary studies, four formulas were selected to evaluate by rheology, spreadability and texture analysis for physical-mechanical characteristics and in vitro scratch assay on cell culture were performed to evaluate the wound healing efficacy of the formulas. The results showed that the ointment belonging to the second group, containing high concentrations of everlasting flower macerate, chamomile flower macerate, fenugreek seed oil and grape seed oil, had the best cell proliferation-wound closure activity. The effectiveness of this formula was examined in an in vivo mouse burn model, and it was observed that it provided more successful wound healing than the control group.