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Effect of short-term mechanical stimulation on the salivary concentrations of vitamin C, vitamin E, total antioxidant capacity and total oxidant status

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dc.contributor.authorLEMES, Aline Louise
dc.contributor.authorAMADEU, Josi Karla
dc.contributor.authorSalazar, Cristina Berrocal
dc.contributor.authorSCHUSSEL, Juliana Lucena
dc.contributor.authorBakkal, MELTEM
dc.contributor.authorAMENÁBAR, Jose Miguel
dc.contributor.institutionauthorBAKKAL, MELTEM
dc.date.accessioned2019-10-05T21:55:01Z
dc.date.available2019-10-05T21:55:01Z
dc.date.issued2018-11-17
dc.description.abstractOxidative stress biomarkers of oral and systemic diseases can be found in saliva. However, there is no uniformity for the saliva collection time in these kinds of analyses and saliva composition may change because of mechanical stimulation. Therefore, the aim of this work was to determine the effect of mechanical stimulation for 10 min on the concentrations of vitamin C, vitamin E, total antioxidants and total oxidants in saliva. Saliva samples from individuals of both sexes, aged between 18 and 38 years, were collected for 10 min at 2 minintervals. Saliva flow rate in each 2 min period was measured, as well the total oxidant state, the total antioxidant capacity, vitamin C and vitamin E concentrations. All analyses were performed in triplicate and were determined using colorimetric tests. The results were analysed using t-test, Friedman-s test and analysis of variance (ANOVA) for repeated measures. Mauchly-s sphericity test was applied and, if necessary, technical corrections were made using the Greenhouse-Geisser test. We found no significant difference between the amounts of saliva produced across the collection times. Total oxidant status, total antioxidant capacity, vitamin C and vitamin E concentrations remained stable. Based on our findings, saliva can be collected for 10 min or less with masticatory stimulation without any variations in the concentration of the variables analysed. However, we suggest using saliva samples after two minutes of mechanical stimulation.
dc.identifier10.1016/j.mri.2007.03.028
dc.identifier.citationLEMES A. L. , AMADEU J. K. , Salazar C. B. , SCHUSSEL J. L. , Bakkal M., AMENÁBAR J. M. , -Effect of short-term mechanical stimulation on the salivary concentrations of vitamin C, vitamin E, total antioxidant capacity and total oxidant status-, SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, cilt.78, ss.556-559, 2018
dc.identifier.doi10.1080/00365513.2018.1517419
dc.identifier.pubmed30278778
dc.identifier.urihttps://hdl.handle.net/20.500.12645/8442
dc.identifier.wosWOS:000454770900006
dc.language.isoen
dc.titleEffect of short-term mechanical stimulation on the salivary concentrations of vitamin C, vitamin E, total antioxidant capacity and total oxidant status
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameMAGNETIC RESONANCE IMAGING
local.avesis.idd284cbbf-dc36-4f0b-8aa5-da04456f6081
local.avesis.response8318
local.publication.isinternational1
relation.isAuthorOfPublication9cf463cf-1704-405b-ac12-ba670d4ab7bb
relation.isAuthorOfPublication.latestForDiscovery9cf463cf-1704-405b-ac12-ba670d4ab7bb
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