Publication:
A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats

Loading...
Thumbnail Image

Date

2014-01-01

Authors

CEBE, T
YANAR, K
ATUKEREN, P
OZAN, T
KURUÇ, AI
KUNBAZ, A
SITAR, ME
MENGI, M
AYDIN, MEHMET
Eşrefoğlu, MUKADDES

Journal Title

Journal ISSN

Volume Title

Publisher

Research Projects

Organizational Units

Journal Issue

Metrics

Search on Google Scholar

Abstract

Age-related myocardial dysfunction has important implications with impaired redox homeostasis. Current study focused on investigation of redox homeostasis and histopathological changes in the myocardium of mimetically (MA), naturally aged (NA), and young control (YC) rats. Chronic D-galactose administration to young male Wistar rats (5 months old) was used to set up experimental aging models. We investigated 16 different oxidative damage biomarkers which have evaluated redox homeostasis of cellular macromolecules such as protein, lipid, and DNA. As a protein oxidation biomarker, advanced oxidation end products, protein carbonyl groups, protein-bound advanced glycation end products, dityrosine, kynurenine, and N-formylkynurenine concentrations in MA and NA rats were found to be significantly higher compared to those in YC rats. On the other hand, the levels of protein thiol groups were not significantly different between groups, whereas lipid peroxidation biomarkers such as conjugated diens, lipid hydroperoxides, and malondialdehyde in MA and NA rats were found to be significantly higher in comparison to those in YCs. For the assessment of oxidative DNA damage, we analyzed eight hydroxy-5'-deoxyguanosine concentrations of MA and NA groups which were higher than YCs. As an antioxidant status in the MA and NA groups, Cu-Zn superoxide dismutase, ferric reducing antioxidant power, and total thiol levels were lower than those in the YCs. Only nonprotein thiol levels were not significantly different. We also observed similar histopathological changes in MA and NA rats. We concluded that the mimetic aging model could be considered as a reliable experimental model for myocardial senescence.

Description

Keywords

Myocardial aging

Citation

CEBE T., YANAR K., ATUKEREN P., OZAN T., KURUÇ A., KUNBAZ A., SITAR M., MENGI M., AYDIN M., Eşrefoğlu M., et al., -A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats.-, Age (Dordrecht, Netherlands), cilt.36, ss.9728, 2014
Page Views

0

File Downloads

11

Sustainable Development Goals