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Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats

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dc.contributor.authorAydemir, Sezgin
dc.contributor.authorAkgün, Sevcan Gül
dc.contributor.authorBECEREN, AYFER
dc.contributor.authorYÜKSEL, MERAL
dc.contributor.authorKUMAŞ, MELTEM
dc.contributor.authorErdogan, Nusret
dc.contributor.authorSardas, Semra
dc.contributor.authorOmurtag, Gulden Zehra
dc.contributor.institutionauthorKUMAŞ, MELTEM
dc.date.accessioned2020-10-29T21:53:34Z
dc.date.available2020-10-29T21:53:34Z
dc.date.issued2017-01-01T00:00:00Z
dc.description.abstractFormaldehyde (FA) is an organic chemical which is widely used all over the world and has hazardous effects for the environment. FA can react with many biomolecules in the biological systems and lead to toxic effects on humans. Melatonin (MEL), a neurohormone produced by pineal gland, has been shown to be an effective antioxidant with free radical scavenging properties. The present study aimed to evaluate the ameliorative effects of MEL on FA-induced toxicity by monitoring oxidant/antioxidant and histopathological changes in the lung, liver and kidney tissues of rats as well as DNA damage in the blood samples. FA was administered through inhalation at a rate of 6 ppm for 6 weeks and intraperitoneal injection at a rate of 10 mg/kg/day for 14 days. MEL was administered in related groups at a rate of 10 mg/kg/day. Upon the completion of the experimental protocol, tissues were dissected for processing biochemical assays and routine histological staining. Blood samples were collected to investigate DNA damage with the comet assay and ELISA kit for 8-hydroxydeoxyguanosine (8-OHdG). FA exposures increased the levels of DNA damage, malondialdehyde and myeloperoxidase activity and reduced glutathione levels. FA also significantly raised the level of tissue reactive oxygen species. FA-induced morphological changes in the tissues were also observed with the light microscope. These alterations were reversed by MEL treatment. In conclusion, the present study suggests that oxidative mechanisms play an important role in FA toxicity. MEL ameliorates oxidative tissue and DNA damage resulting from FA-induced toxicity by balancing oxidant-antioxidant status, inhibiting neutrophil infiltration and reducing 8-OHdG level, and might be beneficial in reducing FA-induced oxidative tissue and DNA damage.
dc.identifier.citationAydemir S., Akgün S. G. , BECEREN A., YÜKSEL M., KUMAŞ M., Erdogan N., Sardas S., Omurtag G. Z. , -Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats-, INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, cilt.10, ss.6250-6261, 2017
dc.identifier.scopus85018414604
dc.identifier.trdizintrdizin
dc.identifier.urihttp://hdl.handle.net/20.500.12645/26460
dc.identifier.wosWOS:000400553200039
dc.titleMelatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats
dc.typeArticle
dspace.entity.typePublication
local.avesis.ida1d016ec-1482-4db2-942f-ea656f4bc7fc
local.publication.isinternational1
relation.isAuthorOfPublication566662cf-8bbb-4744-bfd8-867d0784f978
relation.isAuthorOfPublication.latestForDiscovery566662cf-8bbb-4744-bfd8-867d0784f978
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