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A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study

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dc.contributor.authorGÜL AMUK, NİSA
dc.contributor.authorKURT, GÖKMEN
dc.contributor.authorYANDIM KARTAL, MELIS
dc.contributor.authorADAN, AYSUN
dc.contributor.authorBARAN, YUSUF
dc.contributor.institutionauthorKURT, GÖKMEN
dc.date.accessioned2019-10-05T12:51:53Z
dc.date.available2019-10-05T12:51:53Z
dc.date.issued2018-01-01
dc.description.abstractThe aim of this study was to introduce a minimally invasive procedure for mesenchymal stem cell (MSC) transfer into the intact periodontal ligament (PDL) of the molar teeth in rats. Ten 12-week-old Wistar albino rats were used for this preliminary study. MSCs were obtained from bones of two animals and were labeled with green fluorescent protein (GFP). Four animals were randomly selected for MSC injection, while 4 animals served as a control group. Samples were prepared for histological analysis, Cox-2 mRNA expression polymerase chain reaction analysis, and fluorescent microscopy evaluation. The number of total cells, number of osteoclastic cells, and Cox-2 mRNA expression levels of the periodontal tissue of teeth were calculated. The number of total cells was increased with MSC injections in PDL significantly (P < 0.001). The number of osteoclastic cells and Cox-2 mRNA expression were found to be similar for the two groups. GFP-labeled MSCs were observed with an expected luminescence on the smear samples of the PDL with transferred MSCs. The results of this preliminary study demonstrate successful evidence of transferring MSCs to intact PDL in a nonsurgical way and offer a minimally invasive procedure for transfer of MSCs to periodontal tissues.en
dc.identifier
dc.identifier.citationGÜL AMUK N., KURT G., YANDIM KARTAL M., ADAN A., BARAN Y., -A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study-, Turkish Journal Of Biology, cilt.42, ss.382-391, 2018
dc.identifier.pubmed30930622
dc.identifier.trdizinDOI: https://doi.org/10.3906/biy-1712-62
dc.identifier.trdizintrdizin
dc.identifier.urihttps://hdl.handle.net/20.500.12645/764
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30930622/
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.subjectStem cell
dc.subjectcyclooxygenase-2
dc.subjectGreen fluorescent protein
dc.subjectPeriodontal ligament
dc.subjectTransfer without scaffold
dc.titleA minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameDirim Dergisi
local.avesis.id10b49319-b538-47d8-bff1-5525449d6d02
local.avesis.response634
relation.isAuthorOfPublication3e64bd84-5cc2-4d20-a9d6-bbda78ee6d9f
relation.isAuthorOfPublication.latestForDiscovery3e64bd84-5cc2-4d20-a9d6-bbda78ee6d9f
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