Publication: Achieving the balance: Biphasic effects of genistein on PC-3 cells
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Yildiz, Mehmet Taha
This study examined the response of PC-3 cells to physiological (0.5, 2.5, 5, 10 mu M) and pharmacological (50 mu M) concentrations of genistein which is a main bioactive compound in soy. Following 48 hr genistein treatment, cell-based assays and genome-wide microarray were performed. It was evidenced that maximal physiologically achievable concentrations of genistein (0.5-10 mu M) lead to significant increase in cell viability (p 10 mu M) appeared to have a novel mechanism of action, specifically down-regulating TGF-beta by decreasing specifically SMAD 2/3,4 which are in the downstream TGF-beta signaling cascade. Practical applications This study highlights for the first time that maximal physiologically achievable concentrations of genistein (0.5-10 mu M) have proliferative effects evidenced by alterations in global gene expression patterns of PC-3 cells. Our results particularly represent a closer examination of dietary genistein consumption for the prevention and/or treatment of cancer that maximal physiologically achievable concentrations of genistein could have detrimental effects on individuals with prostate cancer. Further studies as in vivo would be necessary to remove shadows on the effect of genistein on prostate cancer progression.
Terzioglu-Usak S., Yildiz M. T. , Goncu B., Ozten-Kandas N., -Achieving the balance: Biphasic effects of genistein on PC-3 cells-, JOURNAL OF FOOD BIOCHEMISTRY, cilt.43, 2019