Person: İNAL, EBRAR
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Publication Metadata only Bitkisel Droglarda Kalite Kontrol(İksad Yayınevi, 2022-10-01) Kartal M.; Ulusoy Ş.; İnal E.; KARTAL, MURAT; ULUSOY, ŞEYMA; İNAL, EBRARPublication Metadata only Chemical Composition of Different Parts of the Vitex agnus-castus L. Essential Oils and Their In-Vitro Cytotoxic Activities(2023-12-01) İnal E.; Nath E. Ö.; Abudayyak M.; Ulusoy Ş.; İnan H. A.; Cicek M.; Kartal M.; İNAL, EBRAR; ULUSOY, ŞEYMA; KARTAL, MURATThe essential oil (EO) compositions and chemotypes of the important ethnomedicinal plantVitex agnus-castusL. flowers, leaves, and fruits collected from Türkiye (Balıkesir and İstanbul) were identified in the present study. Different parts of theV. agnus-castusEO’sin-vitrocytotoxic effects on the MCF-7 (human breast adenocarcinoma) and A549 (human lung carcinoma) human-origin cell lines were anaylzed in the current study. The composition of hydrodistiled EOs extracted from flowers, leaves, and fruits ofV. agnus-castuswere analyzed by GC–FID/MS. Monoterpene hydrocarbons and oxygenated monoterpene compounds were detected as the predominant component class of theV. agnus-castus. EOs extracted from Balıkesir region were defined as the \"α-pinene-1,8-cineole\" chemotype, while EOs extracted from İstanbul region were defined as the \"sabinene-1,8-cineole\" chemotype. Sesquiterpene hydrocarbons constituted more than 20% of the compounds in the EOs extracted from the flowers. To the best of our knowledge, this study is the first to analyze thein-vitrocytotoxic effects of flowers. This study is also the first to show thein-vitrocytotoxic effects of fruit, the most commonly used part of the plant, EO on the MCF-7 cell line. Balıkesir region’s EOs were observed as more potent -especially the purple flower’s IC50 is about 4.68 µg/mL on the MCF7 cell line- than İstanbul regions, which might be attributed to the higher amount ofα-pinene, caryophyllene, and limonene content. Our results indicated that theV. agnus-castusEOs, which containα-pinene, 1,8-cineole, caryophyllene, and limonene as major components, showed relatively high cytotoxic effects compared to the control groups on the MCF7 and A549 cell lines.Publication Metadata only Mucoadhesive Herbal Gel Formulations For Canker Sores(2024-05-15) Alğın Yapar E.; İnal E.; Kara B. A.; Yıldırım T. S.; Yılmaz F. N.; Özkanca C.; Erdal M. S.; Döşler S.; Kartal M.; İNAL, EBRAR; KARTAL, MURATCanker sores, aphthous stomatitis or mouth ulcer is an ulcerative condition and a painful inflammatory process that is related to the oral mucosa which requires a combined treatment approach of both antimicrobial and wound healing processes. Cistus creticus L. and Inula viscosa (L.) Aiton have both various antimicrobial and antioxidant activities which are beneficial for antisepsis and wound healing. In this study, it was aimed to prepare and optimize mucoadhesive gels including hydroglyceric extracts of C. creticus and I. viscosa either alone or in combination using carboxymethyl cellulose and to evaluate gels by organoleptic, pH, viscosity, spreadability, texture, antimicrobial activity and cytotoxicity-cell proliferation controls. In the first step, the extracts of C. creticus and I. viscosa were analyzed by using LC-HRMS to determine phytoactive contents. Then, formulation optimization studies were carried out, and four different formulations were selected and evaluated in terms of organoleptic, pH, viscosity, spreadability and texture properties. They were found to be successful in terms of these examined parameters. The antimicrobial activities were tested by the microbroth dilution method and the minimum inhibitory concentrations, and results showed that while C. creticus provides antimicrobial efficacy, I. viscosa provides antiviral efficacy for the formulas related to dose- dependent activities against all studied mouth pathogens. Cell proliferation and cytotoxicity test results showed that the most cytotoxic formulation was the one that included C. creticus extract alone while the lowest cytotoxic one was the one that included I. viscosa extract alone, against gingival fibroblast cells. As a conclusion, the formula included 32% combination of hydroglyceric extracts of C. creticus and I. viscosa (1:1) presented antimicrobial and antiviral efficacy with acceptable cytotoxicity, which was found to be encouraging for further studies.Publication Metadata only Design Of A Burn Treatment Ointment Containing Herbal Bioactives(2024-05-15) Alğın Yapar E.; Gökçe E. H.; İnal E.; Ulusoy Ş.; Çankaya İ. İ.; Kartal M.; İNAL, EBRAR; ULUSOY, ŞEYMA; KARTAL, MURATIn this study, it was aimed to develop a burn treatment ointment consisting of herbal ingredients, which combination and synergistic effects had not been evaluated together in a formula before. In this regard, herbal ingredients were determined according to their cell proliferation, anti-inflammatory, antioxidant and astringent activities in light of a comprehensive literature review, and then eight ingredients were selected and divided into two groups. First group formulations were prepared by St. John\"s wort oil (Hypericum perforatum L.), bitter melon seed olive oil macerate (Momordica charantia L.), calendula olive oil macerate (Calendula officialis L.), horse chestnut fruit olive oil macerate (Aesculus hippocastanum L.) and second group formulations were prepared by Golden herb flower olive oil macerate (Helichrysum italicum Roth G.), chamomile flower olive oil macerate (Matricaria recutita L.), fenugreek seed oil (Trigonella foenum graecum L.) and grape seed oil (Vitis vinifera L.) as phytoactive ingredients in various amounts, and white beeswax (Cera alba) and vaseline mixture was used in both groups as ointment bases in various amounts to perform optimization of ointment formulations. At the end of preliminary studies, four formulas were selected to evaluate by rheology, spreadability and texture analysis for physical-mechanical characteristics and in vitro scratch assay on cell culture were performed to evaluate the wound healing efficacy of the formulas. The results showed that the ointment belonging to the second group, containing high concentrations of everlasting flower macerate, chamomile flower macerate, fenugreek seed oil and grape seed oil, had the best cell proliferation-wound closure activity. The effectiveness of this formula was examined in an in vivo mouse burn model, and it was observed that it provided more successful wound healing than the control group.