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dc.contributor.authorOZGUMUS, Osman Birol
dc.contributor.authorAkkoyunlu, YASEMİN
dc.contributor.authorDoymaz, MEHMET ZİYA
dc.contributor.authorIRAZ, Meryem
dc.contributor.authorCICEK, Aysegul Copur
dc.contributor.authorSANDALLI, Cemal
dc.contributor.authorPELEG, Anton Y.
dc.contributor.authorSaral, Aysegul
dc.contributor.authorDuzgun, Azer Ozad
dc.contributor.authorKARAOGLU, Hakan
dc.contributor.authorBERIS, Fatih Saban
dc.date.accessioned2019-10-05T13:17:28Z
dc.date.available2019-10-05T13:17:28Z
dc.date.issued2015-11-01
dc.identifier10.5152/jtgga.2011.62
dc.identifier.urihttps://hdl.handle.net/20.500.12645/1842
dc.description.abstractBackground: The emergence of carbapenem-resistant Klebsiella pneumoniae poses a serious problem to antibiotic management. We investigated the β-lactamases in a group of carbapenem-resistant K. pneumoniae clinical isolates from Turkey. Methods: Thirty-seven strains of K. pneumoniae isolated from various clinical specimens were analyzed by antimicrobial susceptibility testing, PCR for the detection of β-lactamase genes, DNA sequencing, and repetitive extragenic palindronic (REP)-PCR analysis. Results: All 37 isolates were resistant to ampicillin, ampicillin/sulbactam, piperacillin, piperacillin/tazobactam, ceftazidime, cefoperazone/sulbactam, cefepime, imipenem, and meropenem. The lowest resistance rates were observed for colistin (2.7%), tigecycline (11%), and amikacin (19%). According to PCR and sequencing results, 98% (36/37) of strains carried at least one carbapenemase gene, with 32 (86%) carrying OXA-48 and 7 (19%) carrying NDM-1. No other carbapenemase genes were identified. All strains carried a CTX-M-2-like β-lactamase, and some carried SHV- (97%), TEM- (9%), and CTX-M-1-like (62%) β-lactamases. Sequence analysis of bla(TEM) genes identified a bla(TEM-166) with an amino acid change at position 53 (Arg53Gly) from bla(TEM-1b), the first report of a mutation in this region. REP-PCR analysis revealed that there were seven different clonal groups, and temporo-spatial links were identified within these groups. Conclusions: Combinations of β-lactamases were found in all strains, with the most common being OXA-48, SHV, TEM, and CTX-M-type (76% of strains). We have reported, for the first time, a high prevalence of the NDM-1 (19%) carbapenemase in carbapenem-resistant K. pneumoniae from Turkey. These enzymes often co-exist with other β-lactamases, such as TEM, SHV, and CTX-M β-lactamases.en
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.subjectIRAZ M., Duzgun A. O. , SANDALLI C., Doymaz M. Z. , Akkoyunlu Y., Saral A., PELEG A. Y. , OZGUMUS O. B. , BERIS F. S. , KARAOGLU H., et al., -Distribution of beta-Lactamase Genes Among Carbapenem-Resistant Klebsiella pneumoniae Strains Isolated From Patients in Turkey-, ANNALS OF LABORATORY MEDICINE, cilt.35, ss.595-601, 2015
dc.titleDistribution of beta-Lactamase Genes Among Carbapenem-Resistant Klebsiella pneumoniae Strains Isolated From Patients in Turkey
dc.typeArticle
local.avesis.id2c5f27a1-3de5-43a4-88fb-83d0916b730b
local.avesis.response1712
local.article.journalnameJournal of the Turkish German Gynecological Association
dc.identifier.wosWOS:000361642800005
dc.identifier.scopus84942251829
dc.identifier.doi10.3343/alm.2015.35.6.595
dc.identifier.pubmed26354347
local.publication.isinternational1


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