High Performance Liquid Chromatographic Method For The Determination Of Amlodipine And Perindopril
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In the currently work; rapid, sensitive and validated high performance liquidchromatography (HPLC) method for the simultaneous determination of Amlodipin (ADP) andPerindopril (PER) in pure forms and pharmaceutical products were presented. Amlodipine(ADP), 3-O-ethyl 5-O-methyl 2-(2-aminoethoxymethyl)-4-(2-chlorophenyl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate is a vasoselective dihydropyridine calcium antagonist, hasa pharmacokinetic profile that sets it apart from other calcium antagonists (1). Perindopril(PER) (2S,3aS,7aS)-1-[(2S)-2-[[(2S)-1-ethoxy-1-oxopentan-2-yl]amino]propanoyl]-2,3,3a,4,5,6,7,7a-octahydroindole-2-carboxylic acid is an angiotensin-converting enzyme inhibitor thatis used in the treatment of hypertension and heart failure. It is also used to reduce the risk ofcardiovascular events in patients with stable ischemic heart disease (2).Analysis was performed on the Waters Spherisorb® ODS-2 (5μm 150x4.6 mm) column.Mobile phase system has been consisted of acetonitrile : methanol : posphate buffer (65:5:30v/v/v). Specimen was enjected to column at 20 μl. Detection wavelength of 220 nm waspreferred for both compounds and flow rate was 1,5 ml/min. Analysis was completed 5minutes. Column oven temperature was adjusted 30 ºC. The retention time was observed at1.58 and 2.40 min for PER and ADP. Calibration graphs were constructed in the 10- 40μg/ml, 5-40 μg/ml concentration of per, adp respectively. The proposed method wasemployed with a high degree of precision and accuracy in the determination of all activeingredients. The method was suitable for the simultaneous determination and it can be appliedin the quality control department in industries, bio-pharmaceutics and bio-equivalence studies.
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