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Development and validation of a HPLC method for the determination of benzo(a)pyrene in human breast milk

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dc.contributor.authorTekkeli, Serife Evrim Kepekci
dc.contributor.authorGazioglu, IŞIL
dc.contributor.institutionauthorGAZİOĞLU, IŞIL
dc.contributor.institutionauthorTEKKELİ, ŞERİFE EVRİM
dc.date.accessioned2019-10-05T12:39:03Z
dc.date.available2019-10-05T12:39:03Z
dc.date.issued2017-04-01
dc.description.abstractA simple analytical procedure was developed for the quantitation of benzo(a)pyrene in human breast milk using solid phase extraction (SPE) combined with high performance liquid chromatography. Before the chromatographic process, SPE, including C18 functional groups in silicagel cartridges, was conducted for sample preparation. A C18 column (100×4.6 mm id, 3 μm particle size) was used with acetonitrile:water (80:20) as the mobile phase at a flow rate 1mL/min at 30°C. Fluorimetric detection was performed for excitation and emission at 290 and 406 nm, respectively. It was observed that the calibration curve was linear over the range of 0.5-80 ng/mL. The limit of detection and limit of quantitation were found to be 0.5 and 1.07 ng/mL, respectively. Intraday and interday relative standard deviation values were less than 5.15%. Moreover, the newly developed method provides a fast, simple, cost effective, and sensitive assay to detect an important carcinogen substance, benzo(a)pyrene, in human breast milk.en
dc.identifier
dc.identifier.citationGazioglu I., Tekkeli S. E. K. , -Development and validation of a HPLC method for the determination of benzo(a)pyrene in human breast milk-, FOOD SCIENCE AND BIOTECHNOLOGY, cilt.26, ss.319-322, 2017
dc.identifier.doi10.1007/s10068-017-0043-0
dc.identifier.pubmed30263545
dc.identifier.scopus85018481276
dc.identifier.trdizintrdizin
dc.identifier.urihttps://hdl.handle.net/20.500.12645/269
dc.identifier.wosWOS:000399818200003
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleDevelopment and validation of a HPLC method for the determination of benzo(a)pyrene in human breast milk
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameJournal of Clinical and Experimental Investigations (Eski Adı: Klinik ve Deneysel Araştırmalar Dergisi)
local.avesis.id0351a15e-5057-4d76-bec6-131a71302aaa
local.avesis.response139
local.publication.isinternational1
relation.isAuthorOfPublication336380d2-70ee-496d-a002-08059f9da500
relation.isAuthorOfPublication837d5047-762e-43db-b252-9f3d1399378f
relation.isAuthorOfPublication.latestForDiscovery336380d2-70ee-496d-a002-08059f9da500
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