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Characterization of cord blood CD3(+)TCRV alpha 7.2(+)CD161(high) T and innate lymphoid cells in the pregnancies with gestational diabetes, morbidly adherent placenta, and pregnancy hypertension diseases

dc.contributor.authorHaliloglu, Yesim
dc.contributor.authorÖZCAN, ALPER
dc.contributor.authorErdem, Serife
dc.contributor.authorAzizoglu, Zehra Busra
dc.contributor.authorBicer, Ayten
dc.contributor.authorOzarslan, Ozcan Yeniay
dc.contributor.authorKilic, Omer
dc.contributor.authorOkus, Fatma Zehra
dc.contributor.authorDemir, Fatma
dc.contributor.authorCANATAN, HALİT
dc.contributor.authorKARAKÜKCÜ, MUSA
dc.contributor.authorULUDAĞ, Semih Zeki
dc.contributor.authorKÜTÜK, MEHMET SERDAR
dc.contributor.authorUnal, Ekrem
dc.contributor.authorEKEN, AHMET
dc.contributor.institutionauthorKÜTÜK, MEHMET SERDAR
dc.date.accessioned2022-05-10T20:59:18Z
dc.date.available2022-05-10T20:59:18Z
dc.date.issued2022-05-01T00:00:00Z
dc.description.abstractProblem Although pregnant women with gestational diabetes (GD), morbidly adherent placenta (MAP), and pregnancy hypertension (pHT) diseases lead to intrauterine growth restriction (IUGR), little is known about their effect on mucosal-associated invariant T (MAIT) and innate lymphoid cells (ILC) in the umbilical cord. This study aimed to quantify and characterize MAIT cells and ILCs in the cord blood of pregnant women with GD, MAP, and pHT diseases. Method of study Cord blood mononuclear cells (CBMCs) were isolated by Ficoll-Paque gradient. CD3(+)TCRV alpha 7.2(+)CD161(high) cells and ILC subsets were quantified by flow cytometry. CBMCs were stimulated with PMA/Ionomycin and Golgi Plug for 4 h and stained for IFN-gamma, TNF-alpha, and granzyme B. The stained cells were analyzed on FACS ARIA III. Results Compared with healthy pregnancies, in the cord blood of the pHT group, elevated number of lymphocytes was observed. Moreover, the absolute number of IFN-gamma producing CD4(+) or CD4(-) subsets of CD3(+)TCRV alpha 7.2(+)CD161(high) cells as well as those producing granzyme B were significantly elevated in the pHT group compared to healthy controls suggesting increased MAIT cell activity in the pHT cord blood. Similarly, in the MAP group, the absolute number of total CD3(+)TCRV alpha 7.2(+)CD161(high) cells, but not individual CD4(+) or negative subsets, were significantly increased compared with healthy controls- cord blood. Absolute numbers of total CD3(+)TCRV alpha 7.2(+)CD161(high) cells and their subsets were comparable in the cord blood of the GD group compared with healthy controls. Finally, the absolute number of total ILCs and ILC3 subset were significantly elevated in only pHT cord blood compared with healthy controls. Our data also reveal that IFN-gamma(+) or granzyme B+ cell numbers negatively correlated with fetal birth weight. Conclusions CD3(+)TCRV alpha 7.2(+)CD161(high) cells and ILCs show unique expansion and activity in the cord blood of pregnant women with distinct diseases causing IUGR and may play roles in fetal growth restriction.
dc.identifier.citationHaliloglu Y., ÖZCAN A., Erdem S., Azizoglu Z. B. , Bicer A., Ozarslan O. Y. , Kilic O., Okus F. Z. , Demir F., CANATAN H., et al., -Characterization of cord blood CD3(+)TCRV alpha 7.2(+)CD161(high) T and innate lymphoid cells in the pregnancies with gestational diabetes, morbidly adherent placenta, and pregnancy hypertension diseases-, AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2022
dc.identifier.doi10.1111/aji.13555
dc.identifier.pubmed35452164
dc.identifier.urihttp://hdl.handle.net/20.500.12645/30588
dc.identifier.wosWOS:000789116200001
dc.titleCharacterization of cord blood CD3(+)TCRV alpha 7.2(+)CD161(high) T and innate lymphoid cells in the pregnancies with gestational diabetes, morbidly adherent placenta, and pregnancy hypertension diseases
dc.typeArticle
dspace.entity.typePublication
local.avesis.id0b740ff0-5acd-455a-b83e-47c350fbc47c
local.publication.isinternational1
relation.isAuthorOfPublication196d0580-afed-4bc7-9bd9-e0b23cff35bc
relation.isAuthorOfPublication.latestForDiscovery196d0580-afed-4bc7-9bd9-e0b23cff35bc

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