Publication:
The Effect of Selenium on Ischemia-Reperfusion Injury: An Experimental Study on a Transverse Rectus Abdominis Musculocutaneous Flap Model

Simple item page
dc.contributor.authorTenekeci, Goktekin
dc.contributor.authorBilen, Bilge Turk
dc.contributor.authorTÜRKÖZ, YUSUF
dc.contributor.authorSahin, NURHAN
dc.contributor.authorBulam, Nazire
dc.contributor.authorErdemli, Mehmet Erman
dc.contributor.institutionauthorŞAHİN, NURHAN
dc.date.accessioned2020-10-22T15:58:42Z
dc.date.available2020-10-22T15:58:42Z
dc.date.issued2016-01-01T00:00:00Z
dc.description.abstractBackground:The aim of this study is to investigate effects of selenium and enlighten the possible mechanism of action in a rat transverse musculocutaneous flap model following ischemia-reperfusion injury.Materials and Methods:In this study, an experimental model, which mimicked free tissue transfer, was applied. Twenty-four male Wistar Albino rats were divided into a control group (N=12), and a selenium treated group (N=12). A superiorly based transverse rectus abdominis musculocutaneous (TRAM) flap was elevated and an ischemic insult for 4 hours was given. In selenium treated group (Group 2), sodium selenite (0.625mg/kg) was injected intraperitoneally (i.p), 2 hours before the induction of ischemia. Six rats from each group were sacrificed at 24 hours after the operation and malonyldialdehyde (MDA), nitric oxide (NO), and glutathione (GSH) levels were measured biochemically, whereas the intensity of neutrophil infiltration was evaluated. For the rest of the rats in Group 2, sodium selenite was injected at the same dose everyday to the postoperative 10th day, in which the remaining 6 rats from each group were sacrificed. On postoperative 10th day, flap viability was assessed along with the evaluation of intensity of neovascularization.Results:In Group 1, MDA levels were higher significantly (P0.05), and GSH (P>0.05) levels among Group 1 and 2. Neutrophil infiltration was more intense in Group 1, when compared with Group 2 whereas neovascularization was more abundant in samples of Group 2. Group 2 shows higher average flap surface areas when compared with Group 1 (P<0.05).Discussion:The results of this study demonstrated the preventive effect of selenium against ischemia-reperfusion injury by reducing tissue necrosis in muscle flaps possibly by decreasing MDA, increasing neovascularization, and decreasing neutrophil infiltration, thus suppressing inflammation.
dc.identifier.citationTenekeci G., Bilen B. T. , TÜRKÖZ Y., Sahin N., Bulam N., Erdemli M. E. , -The Effect of Selenium on Ischemia-Reperfusion Injury: An Experimental Study on a Transverse Rectus Abdominis Musculocutaneous Flap Model-, JOURNAL OF CRANIOFACIAL SURGERY, cilt.27, ss.242-246, 2016
dc.identifier.doi10.1097/scs.0000000000002178
dc.identifier.scopus84957563112
dc.identifier.urihttp://hdl.handle.net/20.500.12645/23773
dc.identifier.wosWOS:000370075700056
dc.titleThe Effect of Selenium on Ischemia-Reperfusion Injury: An Experimental Study on a Transverse Rectus Abdominis Musculocutaneous Flap Model
dc.typeArticle
dspace.entity.typePublication
local.avesis.id5b6633c5-d60d-4b4e-8215-40a9f59bc260
local.publication.isinternational1
relation.isAuthorOfPublication84f8cc2e-0a04-41e1-b473-7f2af3f72119
relation.isAuthorOfPublication.latestForDiscovery84f8cc2e-0a04-41e1-b473-7f2af3f72119
Files
Collections
Silinecekler