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HLA Class I Expression Changes in Different Types of Cultured Parathyroid Cells.

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dc.contributor.authorGoncu, B
dc.contributor.authorKandas, NO
dc.contributor.authorYucesan, Emrah
dc.contributor.authorAysan, E
dc.contributor.institutionauthorGÖNCÜ, BEYZA SERVET
dc.contributor.institutionauthorYÜCESAN, EMRAH
dc.date.accessioned2020-01-14T21:00:58Z
dc.date.available2020-01-14T21:00:58Z
dc.date.issued2019-04-17T00:00:00Z
dc.description.abstractObjectives: Tissue-specific immunogenicity can be characterized by the determination of human leukocyte antigens (HLA). Parathyroid hyperplasia tissue cells are presumed to have the ability to lose HLA class I expression profile during cultivation, whereas healthy parathyroid cells are presumed to already express HLA class I molecules at low levels. However, there are conflicting results about the expression of HLA class I antigens. In this study, our aim was to evaluate different patterns of HLA class I expression in different parathyroid tissue cells. Materials and Methods: Parathyroid tissue cells were isolated enzymatically and cultured in vitro. Expression of HLA class I (HLA-A, HLA-B, HLA-C) mRNA and protein levels were studied in 7 parathyroid adenomas and 9 parathyroid hyperplasia tissue samples by reverse transcriptase-polymerase chain reaction and Western blot analyses. Results: HLA-A protein expression remained stable in parathyroid adenoma and hyperplasia tissue, but HLA-A mRNA expression decreased in adenoma tissue. In parathyroid hyperplasia tissue, HLA-B protein expression remained stable, although mRNA expres sion levels decreased during cultivation. HLA-C mRNA expression was steady in parathyroid adenoma yet significantly decreased in hyperplasia tissue samples. HLA-C protein expression levels were below 30 pg for both types of parathyroid tissue during cultivation. Conclusions: HLA class I expression levels of para thyroid hyperplasia and adenoma tissue were not found to be similar. Parathyroid hyperplasia tissue is the donor tissue for the treatment of permanent hypoparathyroidism. Therefore, expression patterns of HLA class I are directly relevant to the transplant process. In particular, the HLA region is highly polymorphic, and, as a consequence of this, heterogeneous correlations among HLA-A, HLA-B, and HLA-C expression patterns of parathyroid tissue should be evaluated in detail before transplant for future studies.en
dc.identifier.citationGoncu B., Yucesan E., Aysan E., Kandas N., -HLA Class I Expression Changes in Different Types of Cultured Parathyroid Cells.-, Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation, 2019
dc.identifier.doi10.6002/ect.2018.0388
dc.identifier.pubmed30995898
dc.identifier.urihttps://openaccess.bezmialem.edu.tr/handle/20.500.12645/12812
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleHLA Class I Expression Changes in Different Types of Cultured Parathyroid Cells.
dc.typeArticle
dspace.entity.typePublication
local.avesis.iddd46c6d6-32f6-40bf-a961-42e09a1e2d85
local.publication.isinternational1
relation.isAuthorOfPublication2f62fb6f-8415-4dca-9a79-54a3d131a08a
relation.isAuthorOfPublication9ea2b80f-531b-49a7-83ef-723bfd8584b2
relation.isAuthorOfPublication.latestForDiscovery9ea2b80f-531b-49a7-83ef-723bfd8584b2
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