Publication:
Glioma cancer stem cells modulating the local tumor immune environment.

dc.contributor.authorKhan, Imran
dc.contributor.authorMahfooz, Sadaf
dc.contributor.authorKaracam, Busra
dc.contributor.authorElbasan, Elif Burce
dc.contributor.authorAkdur, Kerime
dc.contributor.authorKarimi, Hasiba
dc.contributor.authorSakarcan, Ayten
dc.contributor.authorHatiboglu, Mustafa Aziz
dc.date.accessioned2023-05-16T14:59:34Z
dc.date.available2023-05-16T14:59:34Z
dc.description.abstractGlioma stem cells (GSCs) drive the resistance mechanism in glioma tumors and mediate the suppression of innate and adaptive immune responses. Here we investigate the expression of mesenchymal-epithelial transition factor (c-Met) and Fas receptor in GSCs and their role in potentiating the tumor-mediated immune suppression through modulation of tumor infiltrating lymphocyte (TIL) population. Tumor tissues were collected from 4 patients who underwent surgery for glioblastoma. GSCs were cultured as neurospheres and evaluated for the co-expression of CD133, c-Met and FasL through flow cytometry. TILs were isolated and evaluated for the lymphocyte subset frequencies including CD3 +, CD4 +, CD8 +, regulatory T cells (FOXP3 + CD25) and microglia (CD11b + CD45) using flow cytometry. Our findings revealed that a significant population of GSCs in all four samples expressed c-Met (89-99%) and FasL (73-97%). A significantly low microglia population was found in local immune cells ranging from 3 to 5%. We did not find a statistically significant correlation between expressions of c-Met + GSC and FasL + GSC with local and systemic immune cells. This may be regarded to the small sample size. The percent c-Met + and FasL + GSC population appeared to be related to percent cytotoxic T cells, regulatory T cells and microglia populations in glioblastoma patients. Further investigation is warranted in a larger sample size.
dc.identifier.doi10.3389/fnmol.2022.1029657
dc.identifier.pubmed36299858
dc.identifier.scopus85140322822
dc.identifier.urihttps://hdl.handle.net/20.500.12645/37895
dc.identifier.wosWOS:000876184700001
dc.language.isoen
dc.subjectglioblastoma
dc.subjectglioma stem cells
dc.subjectsystemic immune response
dc.subjecttumor immune response
dc.subjecttumor microenvironment
dc.titleGlioma cancer stem cells modulating the local tumor immune environment.
dspace.entity.typePublication
local.indexed.atPubMed

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