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- PublicationMetadata onlyTail-Approach-Based Design and Synthesis of Coumarin-Monoterpenes as Carbonic Anhydrase Inhibitors and Anticancer Agents.(2023-02-13T21:00:00Z) Zengin Kurt, Belma; Celebi, Gulsen; Ozturk Civelek, Dilek; Angeli, Andrea; Akdemir, Atilla; Sonmez, Fatih; Supuran, Claudiu TIn this study, sixty novel coumarin-monoterpene compounds were synthesized in two series [thirty-two compounds () bearing a triazole ring in the first series, and twenty-eight compounds () bearing an alkyl chain in the second one]. Their inhibitory effects on the human carbonic anhydrase (hCA) isoforms I, II, IX, and XII and anticancer potentials were determined. All synthesized molecules selectively inhibited CA IX and XII. and were found to be the strongest inhibitors, with values of 1.9 nM against hCA IX. Also, showed the highest inhibitory activity with a value of 4.9 nM against hCA XII. Moreover, their cytotoxic effects on colon adenocarcinoma (HT-29), prostate adenocarcinoma (PC-3), and breast adenocarcinoma (MCF-7) cell lines were evaluated. According to the cytotoxicity results, (IC = 2.48 μM) and (IC = 3.91 μM) exhibited the highest cytotoxicity on the MCF-7 cells, while showed the strongest cytotoxic effect on both PC-3 (IC = 9.40 μM) and HT-29 (IC = 12.10 μM) cell lines. , , and decreased CA IX and CA XII protein expression in HT-29 cells, while and showed the strongest reduction of both CA IX and CA XII in MCF-7 cells. All of the selected compounds increased total apoptosis in a concentration-dependent manner in HT-29 and MCF-7 cells. has the strongest apoptotic effect in MCF-7 cells. increased early apoptosis primarily, while and increased total apoptosis in HT-29. In addition, PI/Hoechst staining proves that apoptotic cells are increased in HT-29 with an effect of , , and . As a result of the modeling studies, it has been shown that only the open coumarin form of the compounds can interact directly with the active-site Zn ion. It has been shown that coumarin-monoterpene structures with different alkyl and monoterpene groups both specifically inhibit CA IX and XII and exhibit specific cytotoxicity in different cell lines.
- PublicationMetadata onlyClinical and anatomical outcomes of isolated coracoclavicular fixation after acromioclavicular joint injury: is it stable enough or is additional horizontal fixation necessary?(2022-12-31T21:00:00Z) Şahin, Koray; Ertogrul, Rodi; Kapıcıoğlu, Mehmet; Erşen, Ali; Bilsel, KeremRecently, an emphasis has been put on anatomical reduction of acromioclavicular (AC) joint both in vertical and hortizontal planes for management of AC joint injuries due to persisting horizontal instability. Therefore, an additional AC fixation in horizontal plane has been recommended. However, relation between horizontal AC joint instability and clinical outcomes is still controversial. This study aims to evaluate outcomes of isolated coraco-clavicular fixation using arthroscopic assisted single coraco-clavicular tunnel technique in grade III and V AC joint injuries and to investigate the correlation between anatomical and clinical outcomes.
- PublicationMetadata onlyPrivacy awareness among healthcare professionals in intensive care unit: A multicenter, cross-sectional study.(2023-02-09T21:00:00Z) Ozdinc, Ahmet; Aydin, Zuleyha; Calim, Muhittin; Ozkan, Ahmet Selim; Bakir, Huseyin; Akbas, SedatThis multicenter, cross-sectional study aimed to determine and examine the privacy awareness and patient rights education of healthcare professionals working in intensive care units (ICUs). The primary purpose of this study was to determine the privacy awareness of healthcare professionals working in the ICU. In addition, the secondary aim was to examine the relationship between patient rights education and awareness scores, as well as to question the need for privacy awareness education. The study population consisted of ICU physicians, nurses, and allied health personnel working in university hospitals, training and research hospitals, state hospitals, and private hospitals in Turkey. The data were collected through a questionnaire prepared by the researchers, including a question set about sociodemographics, a question about patient rights education, and the privacy awareness scale (PAS) scores using online Google Forms. In the results of the study conducted among 569 participants, the mean total PAS score was 38.31 ± 2.54. The PAS score was significantly different according to the occupation. The PAS scores of the nurses were higher than physicians and allied health personnel. The PAS scores differed according to whether the participants had received patient rights education. This study found that nurses were the group with the highest PAS scores among healthcare professionals. In addition, the PAS scores of nurses working in private and training and research hospitals were higher than those of other hospital employees. On the other hand, the lowest scores belonged to university hospitals and receiving patient rights education increased the PAS score of the nurses. This study showed that all enrolled healthcare professionals required in-service training to gain privacy awareness.
- PublicationMetadata onlyInvestigation of serum enzyme activities and oxidative stress markers in preeclampsia: a multiparameter analysis.(2023-03-01T21:00:00Z) Selek, Sahabettin; Gul, Ayse Zehra; Atakul, Nil; Meydan, Sedat; Sarıkaya, Alime; Koktasoglu, Fatmanur; Atamer, YıldızPreeclampsia, a high cause of fetomaternal morbidity-mortality, remains a significant burden affecting 8% of all pregnancies. Environmental conditions induce disease development leading to endothelial dysfunction in genetically predisposed women. Our aim is to discuss oxidative stress as a well-established contributing factor to disease progression with being the first study to show new evidence about serum dehydrogenase enzyme levels (isocitrate, malate, glutamate dehydrogenase) with oxidative markers (myeloperoxidase, total antioxidant-oxidant status, oxidative stress index). Serum parameters were analyzed with photometric method (Abbott ARCHITECT c8000). The enzyme levels and oxidative markers were significantly higher in patients, supporting the redox imbalance in preeclampsia. According to ROC analysis, malate dehydrogenase showed an outstanding diagnostic ability with the highest AUC value of 0.9 and the cut-off value of 51.2 IU/L. Discriminant analysis including malate, isocitrate and glutamate dehydrogenase had predicted preeclampsia with an overall 87.9% accuracy. Considering the above results, we propose that the enzyme levels increase with oxidative stress functioning as antioxidant defense factors. The unique finding of the study is that the serum levels of malate, isocitrate and glutamate dehydrogenase can be used both separately and combined in the early prediction of preeclampsia. As a novel approach, we also offer combining serum isocitrate and glutamate dehydrogenase levels with ALT, AST tests to state liver functions more reliably in patients. Still, larger sample-sized studies investigating enzyme expression levels are required to confirm the recent findings and to reveal underlying mechanisms.
- PublicationMetadata onlyAortic ischemia-reperfusion injury and potency of fluoxetine.(2023-02-28T21:00:00Z) Altan, Mehmet; Yaman, Muhittin Onur; Kervancıoğlu, Gülnaz; Kılıç, Aysu; Demirci, Elif Kervancıoğlu; Bozdoğan Polat, Sıla Hidayet; Karadeniz, Zeliha; Güner, Ibrahim; Yelmen, Nermin; Şahin, GülderenDue to cross-clamping of the aorta during aortic aneurysm surgeries, ischemia-reperfusion (IR) develops, and it may cause damage to the aorta itself or even to remote organs by oxidative stress or inflammation. Fluoxetine (FLX) which might be used in the preoperative period for its tranquilizing effect also has antioxidant effects in short-term use. The purpose of our study is to examine whether FLX protects aorta tissue, against the damage caused by IR.