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Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification

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dc.contributor.authorPukancsik, Maria
dc.contributor.authorMolnar, Petra
dc.contributor.authorOrban, Agnes
dc.contributor.authorButykai, Adam
dc.contributor.authorMarton, Livia
dc.contributor.authorKezsmarki, Istvan
dc.contributor.authorVertessy, Beata G.
dc.contributor.authorKAMIL, MOHD
dc.contributor.authorAbraham, Amanah
dc.contributor.authorALY, AHMED SAYED IBRAHIM
dc.contributor.institutionauthorKAMIL, MOHD
dc.contributor.institutionauthorALY, AHMED SAYED IBRAHıM
dc.date.accessioned2020-01-15T20:36:28Z
dc.date.available2020-01-15T20:36:28Z
dc.date.issued2019-10-01T00:00:00Z
dc.description.abstractThe rotating-crystal magneto-optical diagnostic (RMOD) technique was developed as a sensitive and rapid platform for malaria diagnosis. Herein, we report a detailed in vivo assessment of the synchronized Plasmodium vinckei lentum strain blood-stage infections by the RMOD method and comparing the results to the unsynchronized Plasmodium yoelii 17X-NL (non-lethal) infections. Furthermore, we assess the hemozoin production and clearance dynamics in chloroquine-treated compared to untreated self-resolving infections by RMOD. The findings of the study suggest that the RMOD signal is directly proportional to the hemozoin content and closely follows the actual parasitemia level. The lack of long-term accumulation of hemozoin in peripheral blood implies a dynamic equilibrium between the hemozoin production rate of the parasites and the immune system-s clearing mechanism. Using parasites with synchronous blood stage cycle, which resemble human malaria parasite infections with Plasmodium falciparum and Plasmodium vivax, we are demonstrating that the RMOD detects both hemozoin production and clearance rates with high sensitivity and temporal resolution. Thus, RMOD technique offers a quantitative tool to follow the maturation of the malaria parasites even on sub-cycle timescales.
dc.description.sponsorshipABD Sağlık ve İnsan Hizmetleri Bakanlığı
dc.description.sponsorshipAmerika Birleşik Devletleri Sağlık Bakanlığı
dc.identifier.citationPukancsik M., Molnar P., Orban A., Butykai A., Marton L., Kezsmarki I., Vertessy B. G. , KAMIL M., Abraham A., ALY A. S. I. , -Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification-, BIOMOLECULES, cilt.9, 2019
dc.identifier.doi10.3390/biom9100579
dc.identifier.pubmed31591333
dc.identifier.scopus85073078755
dc.identifier.urihttps://openaccess.bezmialem.edu.tr/handle/20.500.12645/13222
dc.identifier.wosWOS:000497726800071
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleHighly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification
dc.typeArticle
dspace.entity.typePublication
local.avesis.ida3668bb2-a092-4531-b2ee-ea17589cfdae
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublication90dbf8d7-063b-4fdc-9373-f3678809a1ea
relation.isAuthorOfPublication47a38002-6a0b-4c36-b83d-773a943d999d
relation.isAuthorOfPublication.latestForDiscovery90dbf8d7-063b-4fdc-9373-f3678809a1ea
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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