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Direct Polymerization of Proteins

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dc.contributor.authorAlbayrak, CEM
dc.contributor.authorSwartz, James R.
dc.contributor.institutionauthorALBAYRAK, CEM
dc.date.accessioned2021-05-17T20:59:11Z
dc.date.available2021-05-17T20:59:11Z
dc.date.issued2014-06-01T00:00:00Z
dc.description.abstractWe report the synthesis of active polymers of superfolder green fluorescent protein (sfGFP) in one step decamer using Click chemistry. Up to six copies of the non-natural amino acids (nnAAs) p-azido-L-phenylalanine (pAzF) or pp-ropargyloxy-L-phenylalanine (pPaF) were site-specifically inserted into sfGFP by cell-free protein synthesis (CFPS). sfGFP containing two or three copies of these nnAAs were monomer coupled by copper-catalyzed azide alkyne cycloaddition to synthesize linear or branched protein polymers, respectively. The protein polymers retained >= 63% of their specific activity (i.e., fluorescence) after coupling. Polymerization of a concentrated solution of triply substituted sfGFP resulted in fluorescent macromolecular particles. Our method can be generalized to synthesize polymers of a protein or copolymers of any two or more proteins, and the conjugation sites can be determined exactly by standard genetic manipulation. Polymers of proteins and small molecules can also be created with this technology to make a new class of scaffolds or biomaterials.
dc.identifier.citationAlbayrak C., Swartz J. R. , -Direct Polymerization of Proteins-, ACS SYNTHETIC BIOLOGY, cilt.3, sa.6, ss.353-362, 2014
dc.identifier.doi10.1021/sb400116x
dc.identifier.scopus84903179001
dc.identifier.urihttp://hdl.handle.net/20.500.12645/28885
dc.identifier.wosWOS:000337870800004
dc.titleDirect Polymerization of Proteins
dc.typeArticle
dspace.entity.typePublication
local.avesis.id925c3a5a-fa29-4eab-9a56-807d95bb9c10
local.publication.isinternational1
relation.isAuthorOfPublicationced2804c-b9e5-4f25-9926-74404ade4f49
relation.isAuthorOfPublication.latestForDiscoveryced2804c-b9e5-4f25-9926-74404ade4f49
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