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Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity

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dc.contributor.authorBoran, Tuğçe
dc.contributor.authorGünaydın, Ayşenur
dc.contributor.authorJannuzzı, Ayşe Tarbın
dc.contributor.authorÖzçağlı, Eren
dc.contributor.authorAlpertunga, Büket
dc.contributor.institutionauthorGÜNAYDIN AKYILDIZ, AYŞENUR
dc.date.accessioned2020-10-29T21:30:30Z
dc.date.available2020-10-29T21:30:30Z
dc.date.issued2019-09-01T00:00:00Z
dc.description.abstractCelastrol is a natural bioactive compound extracted from the medicinal plant Tripterygium wilfordii Hook F. It exhibits immunosuppressive, anti-inflammatory, and antioxidant activities. Cisplatin is a commonly used chemotherapeutic drug in the treatment of a wide range of tumors. Although very effective therapeutically, it can cause nephrotoxicity leading to dose reduction or discontinuation of treatment. This study aims to clarify the therapeutic potential of celastrol in cisplatin-induced nephrotoxicity. The possible protective effects of celastrol pretreatment against cisplatin-induced oxidative stress and genotoxicity were investigated. A rat kidney epithelial cell line NRK-52E was pretreated with the desired concentrations of celastrol (200 nM, 100 nM, and 50 nM) for 24 h. The cells were treated with 50 mu M cisplatin for a further 24 h to see whether cisplatin caused the same or less toxicity compared to the vehicle control group. Alkaline comet assay was performed for genotoxicity assessment. Genotoxicity evaluation revealed that celastrol caused a statistically significant reduction in DNA damage. Oxidative stress parameters were evaluated by measuring the glutathione (GSH) and protein carbonyl (PC) levels and also by measuring the enzyme activities of glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) enzymes. Celastrol pretreatment increased the GSH content of the cells and ameliorated the protein carbonylation level. Likewise, celastrol pretreatment improved the GR and CAT activities. However, no significant difference was observed in GPx and SOD activities. In the light of these findings, celastrol treatment could be a therapeutic option to reduce cisplatin-induced nephrotoxicity. Further studies are needed for the clarification of its therapeutic potential.
dc.description.sponsorshipİstanbul Üniversitesi
dc.description.sponsorshipİstanbul Üniversitesi
dc.identifier.citationBoran T., Günaydın A., Jannuzzı A. T. , Özçağlı E., Alpertunga B., -Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity-, TOXICOLOGY RESEARCH, cilt.8, ss.723-730, 2019
dc.identifier.doi10.1039/c9tx00141g
dc.identifier.pubmed31588349
dc.identifier.scopus85071605779
dc.identifier.urihttp://hdl.handle.net/20.500.12645/26309
dc.identifier.wosWOS:000484536100012
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleCelastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity
dc.typeArticle
dspace.entity.typePublication
local.avesis.id8cbbd64a-d647-475e-a481-e641c5db91c4
local.publication.isinternational1
relation.isAuthorOfPublication98a70592-2158-4e37-b8d7-80d3bd64c98c
relation.isAuthorOfPublication.latestForDiscovery98a70592-2158-4e37-b8d7-80d3bd64c98c
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