Publication:
Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis

dc.contributor.authorKoyuncu, Ismail
dc.contributor.authorKocyigit, ABDÜRRAHİM
dc.contributor.authorOzer, Ahmet
dc.contributor.authorSELEK, Sahabettin
dc.contributor.authorKirmit, Adnan
dc.contributor.authorKarsen, Hasan
dc.contributor.institutionauthorKOÇYİĞİT, ABDÜRRAHİM
dc.contributor.institutionauthorSELEK, ŞAHABETTİN
dc.date.accessioned2019-10-05T15:12:15Z
dc.date.available2019-10-05T15:12:15Z
dc.date.issued2018-01-01
dc.description.abstractSerologic tests for brucellosis aim to detect antibodies produced against membranous lipopolysaccharide of bacteria. Diagnostic use of this method is limited due to false positiveness. This study evaluates an alternative antigen to lipopolysaccharides (LPS), outer membrane 28-precursor-protein, of Brucella melitensis Rev1 for its diagnostic value. Omp28 precursor of B. melitensis Rev1 was cloned, expressed, and purified. 6-His and sumo epitope tags were used to tag the protein at N-termini. Omp28 gene was amplified based on the ORF sequence and cloned into a pETSUMO vector. The recombinant construct was propagated in Escherichia coli One Shot® Mach1™ cells then transformed into E. coli BL21(D3) cells for protein expression. The purified protein was studied in an indirect ELISA for diagnosis of brucellosis. Sera samples from 60 patients were screened by ELISA and the results were compared to Rose Bengal plate test. Recombinant antigen-based iELISA has given a successful outcome with the sensitivity, specificity, positive predictive value, and negative predictive value of 87.8%, 96.2%, 96.6%, and 78.78%, respectively. In conclusion, recombinant production and purification of the immunodominant Omp28 precursor protein has been achieved successfully in a one-step process with efficient yield and can be used for diagnosis of brucellosis in humansen
dc.identifier.citationKoyuncu I., Kocyigit A., Ozer A., SELEK S., Kirmit A., Karsen H., -Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis-, Central European Journal of Immunology, cilt.43, ss.81-89, 2018
dc.identifier.pubmed29736150
dc.identifier.trdizintrdizin
dc.identifier.urihttps://hdl.handle.net/20.500.12645/5721
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleDiagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameActa paediatrica Taiwanica = Taiwan er ke yi xue hui za zhi
local.avesis.id8d43cb72-e67e-4b72-bded-9b2abe6c9d98
local.avesis.response5591
relation.isAuthorOfPublication75babbd2-f46b-4121-aa00-54d883a6f907
relation.isAuthorOfPublication8eb7d539-8ea4-435c-830e-8ef630342c7d
relation.isAuthorOfPublication.latestForDiscovery8eb7d539-8ea4-435c-830e-8ef630342c7d
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