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An in vitro analysis of an innovative standardized phospholipid carrier-based Melissa officinalis L. extract as a potential neuromodulator for emotional distress and related conditions

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dc.contributor.authorKARA M.
dc.contributor.authorSahin S.
dc.contributor.authorRabbani F.
dc.contributor.authorÖZTAŞ E.
dc.contributor.authorHasbal-Celikok G.
dc.contributor.authorKANIMDAN E.
dc.contributor.authorKOÇYİĞİT A.
dc.contributor.authorKanwal A.
dc.contributor.authorWade U.
dc.contributor.authorYakunina A.
dc.contributor.authoret al.
dc.contributor.institutionauthorKANIMDAN, EBRU
dc.contributor.institutionauthorKOÇYİĞİT, ABDÜRRAHİM
dc.date.accessioned2024-04-16T21:50:13Z
dc.date.available2024-04-16T21:50:13Z
dc.date.issued2024-01-01
dc.description.abstractBackground: Melissa officinalis L. (MO), commonly known as lemon balm, a member of the mint family, is considered a calming herb. In various traditional medicines, it has been utilized to reduce stress and anxiety and promote sleep. A growing body of clinical evidence suggests that MO leaf extract supplementation possesses considerable neuropharmacological properties. However, its possible mechanism of action largely remains unknown. Objective: In the present in vitro studies, we comparatively investigated the central nervous system (CNS)-calming and antioxidative stress properties of an innovative standardized phospholipid carrier-based (Phytosome™) MO extract (Relissa™) vs. an unformulated dry MO extract. Methods: The neuropharmacological effect of the extract was studied in the anti-depressant enzymes γ-aminobutyrate transaminase (GABA-T) and monoamine oxidase A (MAO-A) assays and SH-SY5Y cells brain-derived neurotrophic factor (BDNF) expression assay. The neuroprotective effect of the extract against oxidative stress was assessed in SH-SY5Y cell-based (H2O2-exposed) Total Antioxidant Status (TAS) and Total Reactive Oxygen Species (ROS) assays. The cytotoxic effect of the extract was evaluated using MTT and LDH assays. The extract antioxidant effect was also evaluated in cell-free chemical tests, including TEAC-ABTS, DPPH, Ferric Reducing Antioxidant Power (FRAP), Oxygen Radical Antioxidant Capacity (ORAC), and Hydroxyl Radical Antioxidant Capacity (HORAC) assays. Results: Relissa™ exhibited high GABA-T inhibitory activity, IC50 (mg/mL) = 0.064 vs. unformulated dry MO extract, IC50 (mg/mL) = 0.27. Similar inhibitory effects were also observed for MAO-A. Relissa™ demonstrated an improved neuroprotective antioxidant effect on SH-SY5Y cells against H2O2-induced oxidative stress. Compared to unformulated dry MO extract, Relissa™ exerted high protective effect on H2O2-exposed SH-SY5Y cells, leading to higher cells BDNF expression levels. Moreover, cell-free chemical tests, including TEAC-ABTS, DPPH radical scavenging, FRAP, ORAC, and HORAC assays, validated the improved antioxidant effect of Relissa™ vs. unformulated dry MO extract. Conclusion: The results of the present study support the neuromodulating and neuroprotective properties of Relissa™, and its supplementation may help in the amelioration of emotional distress and related conditions.
dc.identifier.citationKARA M., Sahin S., Rabbani F., ÖZTAŞ E., Hasbal-Celikok G., KANIMDAN E., KOÇYİĞİT A., Kanwal A., Wade U., Yakunina A., et al., "An in vitro analysis of an innovative standardized phospholipid carrier-based Melissa officinalis L. extract as a potential neuromodulator for emotional distress and related conditions", Frontiers in Molecular Biosciences, cilt.11, 2024
dc.identifier.doi10.3389/fmolb.2024.1359177
dc.identifier.issn2296-889X
dc.identifier.pubmed38545418
dc.identifier.scopus85188715469
dc.identifier.urihttps://avesis.bezmialem.edu.tr/api/publication/578309c5-e49e-4b34-a3ec-6a8706a42def/file
dc.identifier.urihttps://hdl.handle.net/20.500.12645/39217
dc.identifier.volume11
dc.identifier.wosWOS:001191134200001
dc.relation.ispartofFrontiers in Molecular Biosciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectCytogenetic
dc.subjectNatural Sciences
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectLife Sciences (LIFE)
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectBIOCHEMISTRY & MOLECULAR BIOLOGY
dc.subjectBiyokimya
dc.subjectMoleküler Biyoloji
dc.subjectBiyokimya, Genetik ve Moleküler Biyoloji (çeşitli)
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectcalming agent
dc.subjectMelissa officinalisL
dc.subjectmonoamine oxidase A (MAO-A)
dc.subjectPhytosome™
dc.subjectRelissa™
dc.subjectγ-aminobutyrate (GABA)
dc.subjectγ-aminobutyrate transaminase (GABA-T)
dc.titleAn in vitro analysis of an innovative standardized phospholipid carrier-based Melissa officinalis L. extract as a potential neuromodulator for emotional distress and related conditions
dc.typearticle
dspace.entity.typePublication
local.avesis.id578309c5-e49e-4b34-a3ec-6a8706a42def
relation.isAuthorOfPublication946182e1-ffcc-4713-9837-737d842b49f3
relation.isAuthorOfPublication75babbd2-f46b-4121-aa00-54d883a6f907
relation.isAuthorOfPublication.latestForDiscovery946182e1-ffcc-4713-9837-737d842b49f3
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