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Novel approaches for determination of antiretroviral reverse transcriptase inhibitor agent in commercial dosage forms by using spectrofluorimetric, first derivative spectrophotometric, and HPLC methods

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dc.contributor.authorTiris, Gizem
dc.contributor.authorCiloglu, Emine
dc.contributor.authorERK, NEVİN
dc.contributor.institutionauthorTIRIS, GİZEM
dc.date.accessioned2020-10-22T15:53:32Z
dc.date.available2020-10-22T15:53:32Z
dc.date.issued2020-09-01T00:00:00Z
dc.description.abstractBackground: Rapid, simple, and sensitive spectrofluorimetric, first derivative spectrophotometric, and high-performance liquid chromatographic (HPLC) methods have been developed and validated for determination of tenofovir in pharmaceutical preparations. Spectrofluorimetric method is based on measuring the native fluorescence intensity of tenofovir at 375.0 nm after excitation at 275.0 nm. Calibration graphics were plotted and were found linear over 4.72-15.75 mu g/mL concentration range (r(2)= 0.9994). The second method developed was the first derivative spectrophotometric method for the analysis of tenofovir performed by measuring the amplitude at 251.7 and 272.6 nm. Linearity was observed in the concentration range 10.0-28.0 mu g/mL (r(2)= 0.9998). On the other hand, HPLC with a diode array detector (DAD). Ritonavir was used as internal standard (IS). HPLC analysis was carried out on a C(18)column (Wakosil-II 5 C-18 AR, 4.6 x 250 mm) using a mobile phase consisting of acetonitrile: 0.5% formic acid (99.5:0.5; v/v) at a flow rate of 1.0 mL/min. Injection volume was 5.0 mu L. DAD signals at 260.0 nm were used. HPLC method was found to be linear over the concentration range of 10.0-100.0 mu g/mL (r(2)= 0.9990).
dc.identifier.citationTiris G., Ciloglu E., ERK N., -Novel approaches for determination of antiretroviral reverse transcriptase inhibitor agent in commercial dosage forms by using spectrofluorimetric, first derivative spectrophotometric, and HPLC methods-, FUTURE JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.6, 2020
dc.identifier.doi10.1186/s43094-020-00070-w
dc.identifier.urihttp://hdl.handle.net/20.500.12645/23585
dc.identifier.wosWOS:000568353900001
dc.titleNovel approaches for determination of antiretroviral reverse transcriptase inhibitor agent in commercial dosage forms by using spectrofluorimetric, first derivative spectrophotometric, and HPLC methods
dc.typeArticle
dspace.entity.typePublication
local.avesis.id428cdb26-03b0-48b5-9ea1-a5d5a4de944e
local.publication.isinternational1
relation.isAuthorOfPublication1ab47475-d54a-4b46-a679-47fb775bc1ce
relation.isAuthorOfPublication.latestForDiscovery1ab47475-d54a-4b46-a679-47fb775bc1ce
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