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Correlation of host inflammatory cytokines and immune-related metabolites, but not viral NS1 protein, with disease severity of dengue virus infection

dc.contributor.authorSoe, Hui Jen
dc.contributor.authorManikam, Rishya
dc.contributor.authorRaju, Chandramathi Samudi
dc.contributor.authorKhan, MOHAMMAD ASİF
dc.contributor.authorSekaran, Shamala Devi
dc.contributor.institutionauthorKHAN, MOHAMMAD ASİF
dc.date.accessioned2021-08-31T20:59:30Z
dc.date.available2021-08-31T20:59:30Z
dc.date.issued2020-08-01T00:00:00Z
dc.description.abstractSevere dengue can be lethal caused by manifestations such as severe bleeding, fluid accumulation and organ impairment. This study aimed to investigate the role of dengue non-structural 1 (NS1) protein and host factors contributing to severe dengue. Electrical cell-substrate impedance sensing system was used to investigate the changes in barrier function of microvascular endothelial cells treated NS1 protein and serum samples from patients with different disease severity. Cytokines and metabolites profiles were assessed using a multiplex cytokine assay and liquid chromatography mass spectrometry respectively. The findings showed that NS1 was able to induce the loss of barrier function in microvascular endothelium in a dose dependent manner, however, the level of NS1 in serum samples did not correlate with the extent of vascular leakage induced. Further assessment of host factors revealed that cytokines such as CCL2, CCL5, CCL20 and CXCL1, as well as adhesion molecule ICAM-1, that are involved in leukocytes infiltration were expressed higher in dengue patients in comparison to healthy individuals. In addition, metabolomics study revealed the presence of deregulated metabolites involved in the phospholipid metabolism pathway in patients with severe manifestations. In conclusion, disease severity in dengue virus infection did not correlate directly with NS1 level, but instead with host factors that are involved in the regulation of junctional integrity and phospholipid metabolism. However, as the studied population was relatively small in this study, these exploratory findings should be confirmed by expanding the sample size using an independent cohort to further establish the significance of this study.
dc.identifier.citationSoe H. J. , Manikam R., Raju C. S. , Khan M. A. , Sekaran S. D. , -Correlation of host inflammatory cytokines and immune-related metabolites, but not viral NS1 protein, with disease severity of dengue virus infection-, PLOS ONE, cilt.15, sa.8, 2020
dc.identifier.doi10.1371/journal.pone.0237141
dc.identifier.scopus85089301060
dc.identifier.urihttp://hdl.handle.net/20.500.12645/29327
dc.identifier.wosWOS:000561029000002
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleCorrelation of host inflammatory cytokines and immune-related metabolites, but not viral NS1 protein, with disease severity of dengue virus infection
dc.typeArticle
dspace.entity.typePublication
local.avesis.ide86a4b05-8a85-454c-8fd3-4186b2af6acb
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
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relation.isAuthorOfPublication.latestForDiscovery60fee672-8864-4711-ae92-5b9dd3bb0ef7
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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