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Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from <i>Plasmodium</i> Challenge in a Mouse Model.

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dc.contributor.authorReeder, SM
dc.contributor.authorReuschel, EL
dc.contributor.authorBah, MA
dc.contributor.authorYun, K
dc.contributor.authorTursi, NJ
dc.contributor.authorKim, KY
dc.contributor.authorChu, J
dc.contributor.authorZaidi, FI
dc.contributor.authorYilmaz, I
dc.contributor.authorHart, RJ
dc.contributor.authorPerrin, B
dc.contributor.authorXu, Z
dc.contributor.authorHumeau, L
dc.contributor.authorWeiner, DB
dc.contributor.authorAly, Ahmed Sayed Ibrahım
dc.contributor.institutionauthorALY, AHMED SAYED IBRAHıM
dc.date.accessioned2020-01-23T20:59:14Z
dc.date.available2020-01-23T20:59:14Z
dc.date.issued2020-01-10T00:00:00Z
dc.description.abstractThe need for a malaria vaccine is indisputable. A single vaccine for Plasmodium pre-erythrocytic stages targeting the major sporozoite antigen circumsporozoite protein (CSP) has had partial success. Additionally, CD8+ T cells targeting liver-stage (LS) antigens induced by live attenuated sporozoite vaccines were associated with protection in human challenge experiments. To further evaluate protection mediated by LS antigens, we focused on exported pre-erythrocytic proteins (exported protein 1 (EXP1), profilin (PFN), exported protein 2 (EXP2), inhibitor of cysteine proteases (ICP), transmembrane protein 21 (TMP21), and upregulated in infective sporozoites-3 (UIS3)) expressed in all Plasmodium species and designed optimized, synthetic DNA (synDNA) immunogens. SynDNA antigen cocktails were tested with and without the molecular adjuvant plasmid IL-33. Immunized animals developed robust T cell responses including induction of antigen-specific liver-localized CD8+ T cells, which were enhanced by the co-delivery of plasmid IL-33. In total, 100% of mice in adjuvanted groups and 71%–88% in non-adjuvanted groups were protected from blood-stage disease following Plasmodium yoelii sporozoite challenge. This study supports the potential of synDNA LS antigens as vaccine components for malaria parasite infection.en
dc.description.sponsorshipTürkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tubitak )
dc.identifier.citationReeder S., Reuschel E., Bah M., Yun K., Tursi N., Kim K., Chu J., Zaidi F., Yilmaz I., Hart R., et al., -Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from <i>Plasmodium</i> Challenge in a Mouse Model.-, Vaccines, cilt.8, 2020
dc.identifier.doi10.3390/vaccines8010021
dc.identifier.pubmed31936739
dc.identifier.urihttps://openaccess.bezmialem.edu.tr/handle/20.500.12645/13486
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleSynthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from <i>Plasmodium</i> Challenge in a Mouse Model.
dc.typeArticle
dspace.entity.typePublication
local.avesis.idb03612d7-aebc-4dcc-9846-d308611f7f4b
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublication47a38002-6a0b-4c36-b83d-773a943d999d
relation.isAuthorOfPublication.latestForDiscovery47a38002-6a0b-4c36-b83d-773a943d999d
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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