Publication: Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from <i>Plasmodium</i> Challenge in a Mouse Model.
dc.contributor.author | Reeder, SM | |
dc.contributor.author | Reuschel, EL | |
dc.contributor.author | Bah, MA | |
dc.contributor.author | Yun, K | |
dc.contributor.author | Tursi, NJ | |
dc.contributor.author | Kim, KY | |
dc.contributor.author | Chu, J | |
dc.contributor.author | Zaidi, FI | |
dc.contributor.author | Yilmaz, I | |
dc.contributor.author | Hart, RJ | |
dc.contributor.author | Perrin, B | |
dc.contributor.author | Xu, Z | |
dc.contributor.author | Humeau, L | |
dc.contributor.author | Weiner, DB | |
dc.contributor.author | Aly, Ahmed Sayed Ibrahım | |
dc.contributor.institutionauthor | ALY, AHMED SAYED IBRAHıM | |
dc.date.accessioned | 2020-01-23T20:59:14Z | |
dc.date.available | 2020-01-23T20:59:14Z | |
dc.date.issued | 2020-01-10T00:00:00Z | |
dc.description.abstract | The need for a malaria vaccine is indisputable. A single vaccine for Plasmodium pre-erythrocytic stages targeting the major sporozoite antigen circumsporozoite protein (CSP) has had partial success. Additionally, CD8+ T cells targeting liver-stage (LS) antigens induced by live attenuated sporozoite vaccines were associated with protection in human challenge experiments. To further evaluate protection mediated by LS antigens, we focused on exported pre-erythrocytic proteins (exported protein 1 (EXP1), profilin (PFN), exported protein 2 (EXP2), inhibitor of cysteine proteases (ICP), transmembrane protein 21 (TMP21), and upregulated in infective sporozoites-3 (UIS3)) expressed in all Plasmodium species and designed optimized, synthetic DNA (synDNA) immunogens. SynDNA antigen cocktails were tested with and without the molecular adjuvant plasmid IL-33. Immunized animals developed robust T cell responses including induction of antigen-specific liver-localized CD8+ T cells, which were enhanced by the co-delivery of plasmid IL-33. In total, 100% of mice in adjuvanted groups and 71%–88% in non-adjuvanted groups were protected from blood-stage disease following Plasmodium yoelii sporozoite challenge. This study supports the potential of synDNA LS antigens as vaccine components for malaria parasite infection. | en |
dc.description.sponsorship | Türkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tubitak ) | |
dc.identifier.citation | Reeder S., Reuschel E., Bah M., Yun K., Tursi N., Kim K., Chu J., Zaidi F., Yilmaz I., Hart R., et al., -Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from <i>Plasmodium</i> Challenge in a Mouse Model.-, Vaccines, cilt.8, 2020 | |
dc.identifier.doi | 10.3390/vaccines8010021 | |
dc.identifier.pubmed | 31936739 | |
dc.identifier.uri | https://openaccess.bezmialem.edu.tr/handle/20.500.12645/13486 | |
dc.language.iso | en | |
dc.rights | info:eu-repo/semantics/openAccess | en |
dc.title | Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from <i>Plasmodium</i> Challenge in a Mouse Model. | |
dc.type | Article | |
dspace.entity.type | Publication | |
local.avesis.id | b03612d7-aebc-4dcc-9846-d308611f7f4b | |
local.indexed.at | PubMed | |
local.publication.goal | 03 - Sağlık ve Kaliteli Yaşam | |
local.publication.isinternational | 1 | |
relation.isAuthorOfPublication | 47a38002-6a0b-4c36-b83d-773a943d999d | |
relation.isAuthorOfPublication.latestForDiscovery | 47a38002-6a0b-4c36-b83d-773a943d999d | |
relation.isGoalOfPublication | 9c198c48-b603-4e2f-8366-04edcfc1224c | |
relation.isGoalOfPublication.latestForDiscovery | 9c198c48-b603-4e2f-8366-04edcfc1224c |
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