Publication:
Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications

Placeholder

Organizational Units

Program

Authors

Atak, Besir Hakan
Buyuk, Berna
Huysal, Merve
Isik, Sevim
Senel, Mehmet
Metzger, Wolfgang
ÇETİN, GÜVEN

Advisor

Language

Publisher

Journal Title

Journal ISSN

Volume Title

Abstract

In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS + nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS + nHAP-NH2) scaffolds- were synthesized for bone tissue engineering (BTE) purposes. As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS + nHAP scaffolds losing up to 15% of their initial weight, while the CTS + nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment. In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS + nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs). Finally, during osteoinductivity assessment, CTS + nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS + nHAP scaffolds only doubled. These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS + nHAP-NH2 scaffold being the most promising and applicable one. In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes. (C) 2017 Elsevier Ltd. All rights reserved.

Description

Source:

Keywords:

Keywords

Citation

Atak B. H. , Buyuk B., Huysal M., Isik S., Senel M., Metzger W., ÇETİN G., -Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications-, CARBOHYDRATE POLYMERS, cilt.164, ss.200-213, 2017

Endorsement

Review

Supplemented By

Referenced By

1

Views

0

Downloads

View PlumX Details


Sustainable Development Goals