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Broadening Horizons and Teaching Basic Biology Through Cell-Free Synthesis of Green Fluorescent Protein in a High School Laboratory Course

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dc.contributor.authorAlbayrak, CEM
dc.contributor.authorJones, K. C.
dc.contributor.authorSwartz, James R.
dc.contributor.institutionauthorALBAYRAK, CEM
dc.date.accessioned2021-05-17T20:59:13Z
dc.date.available2021-05-17T20:59:13Z
dc.date.issued2013-12-01T00:00:00Z
dc.description.abstractCell-free protein synthesis (CFPS) has emerged as a practical method for producing a broad variety of proteins. In addition, the direct accessibility to the reaction environment makes CFPS particularly suitable as a learning vehicle for fundamental biological concepts. Here, we describe its implementation as a teaching tool for a high school laboratory course. Ninety students in a biotechnology class used CFPS to study the effects of the concentrations of amino acids, cell extract, DNA, and the energy source on accumulation of active super-folder green fluorescent protein. Students estimated product concentrations simply by comparing solution colors to a printed green color gradient. This simple and inexpensive method allows for immediate measurements, and 26 of the 30 groups observed measurable product concentrations within 60 min. These student-generated data were then discussed to illustrate concepts of data analysis such as outliers and standard deviation. We also combined the laboratory experience with a visit to a university campus that included a laboratory tour and a college-style lecture. Our overall objective was to excite the students about the scientific enterprise and to instill a sense of personal relevance and attainability so that these students could realistically consider technical careers.
dc.identifier.citationAlbayrak C., Jones K. C. , Swartz J. R. , -Broadening Horizons and Teaching Basic Biology Through Cell-Free Synthesis of Green Fluorescent Protein in a High School Laboratory Course-, JOURNAL OF SCIENCE EDUCATION AND TECHNOLOGY, cilt.22, sa.6, ss.963-973, 2013
dc.identifier.doi10.1007/s10956-013-9442-z
dc.identifier.scopus84887619609
dc.identifier.urihttp://hdl.handle.net/20.500.12645/28887
dc.identifier.wosWOS:000327090700011
dc.titleBroadening Horizons and Teaching Basic Biology Through Cell-Free Synthesis of Green Fluorescent Protein in a High School Laboratory Course
dc.typeArticle
dspace.entity.typePublication
local.avesis.idc6d970ce-dbe5-4fe0-81eb-e1e4be924299
local.publication.isinternational1
relation.isAuthorOfPublicationced2804c-b9e5-4f25-9926-74404ade4f49
relation.isAuthorOfPublication.latestForDiscoveryced2804c-b9e5-4f25-9926-74404ade4f49
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