Fabric phase sorptive extraction combined with high performance liquid chromatography for the determination of favipiravir in human plasma and breast milk

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Gazioglu I.
Furton K. G.
Kabir A.
Locatelli M.
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A fast procedure obtained by the combination of fabric phase extraction (FPSE) with high performance liquid chromatography (HPLC) has been developed and validated for the quantification of favipiravir (FVP) in human plasma and breast milk. A sol-gel polycaprolactone-block-polydimethylsiloxane-block-polycaprolactone (sol-gel PCAP-PDMS-PCAP) coated on 100% cellose cotton fabric was selected as the most efficient membrane for FPSE in human plasma and breast milk samples. HPLC-UV analysis were performed using a RP C18 column under isocratic conditions. Under these optimezed settings, the overall chromatographic analysis time was limited to only 5 min without encountering any observable matrix interferences. Following the method validation pro-cedure, the herein assay shows a linear calibration curve over the range of 0.2-50 mu g/mL and 0.5-25 mu g/mL for plasma and breast milk, respectively. The method sensitivities in terms of limit of detection (LOD) and limit of quantification (LOQ), validated in both the matrices, have been found to be 0.06 and 0.2 mu g/mL for plasma and 0.15 and 0.5 mu g/mL for milk, respectively. Intraday and interday precision and trueness, accordingly to the International Guidelines, were validated and were below 3.61% for both the matrices. The herein method was further tested on real samples in order to highlight the applicability and the advantage for therapeutic drug monitoring (TDM) applications. To the best of our knowledge, this is the first validated FPSE-HPLC-UV method in human plasma and breast milk for TDM purposes applied on real samples. The validated method provides fast, simple, cost reduced, and sensitive assay for the direct quantification of favipiravir in real biological matrices, also appliyng a well-known rugged and cheap instrument configuration.
Eczacılık, Temel Eczacılık Bilimleri, Yaşam Bilimleri, Kimya, Analitik Kimya, Sağlık Bilimleri, Temel Bilimler, Pharmacology and Therapeutics, Basic Pharmaceutics Sciences, Life Sciences, Chemistry, Analytical Chemistry, Health Sciences, Natural Sciences, KİMYA, ANALİTİK, Temel Bilimler (SCI), FARMAKOLOJİ VE ECZACILIK, Farmakoloji ve Toksikoloji, Yaşam Bilimleri (LIFE), CHEMISTRY, ANALYTICAL, CHEMISTRY, Natural Sciences (SCI), PHARMACOLOGY & PHARMACY, PHARMACOLOGY & TOXICOLOGY, Life Sciences (LIFE), Farmakoloji, Farmakoloji, Toksikoloji ve Eczacılık (çeşitli), Genel Farmakoloji, Toksikoloji ve Eczacılık, Farmakoloji (tıbbi), İlaç Rehberleri, Kimya (çeşitli), Genel Kimya, Filtrasyon ve Ayırma, Fizik Bilimleri, Pharmacy, Pharmacology, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), General Pharmacology, Toxicology and Pharmaceutics, Pharmacology (medical), Drug Guides, Chemistry (miscellaneous), General Chemistry, Filtration and Separation, Physical Sciences, HPLC, Favipiravir, Fabric phase sorptive extraction, Plasma, Breast milk, TDM
TIRIS G., Gazioglu I., Furton K. G., Kabir A., Locatelli M., "Fabric phase sorptive extraction combined with high performance liquid chromatography for the determination of favipiravir in human plasma and breast milk", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.223, 2023
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