Person: GAZİOĞLU, IŞIL
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Publication Synthesis, anticholinesterase activity and molecular modeling study of novel carbamate-substituted thymol/carvacrol derivatives(2017-02-15) KURT, Belma Zengin; Gazioglu, IŞIL; Dag, AYDAN; Salmas, Ramin Ekhteiari; Kayik, Gulru; Durdagi, Serdar; Sonmez, Fatih; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; DAĞ, AYDANNew thymol and carvacrol derivatives with the carbamate moiety were synthesized and their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were evaluated. 5-isopropyl-2-methylphenyl(3-fluorophenyl)carbamate (29) was found to be the most potent AChE inhibitor with IC50 values of 2.22 mu M, and 5-isopropyl-2-methylphenyl (4-fluorophenyl)carbamate (30) exhibited the strongest inhibition against BuChE with IC50 value of 0.02 mu M. Additionally, the result of H4IIE hepatoma cell toxicity assay for compounds 18, 20, 29, 30 and 35 showed negligible cell death at 0.07-10 mu M. Moreover in order to better understand the inhibitory profiles of these molecules, molecular modeling studies were applied. Binding poses of studied compounds at the binding pockets of AChE and BuChE targets were determined. Predicted binding energies of these compounds as well as structural and dynamical profiles of molecules at the target sites were estimated using induced fit docking (IFD) algorithms and post-processing molecular dynamics (MD) simulations methods (i.e., Molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) approaches). (C) 2016 Elsevier Ltd. All rights reserved.Publication HPLC Method for Moxifloxacin in Breast Milk By Fluorimetric Detection with Precolumn Derivatization(2015-10-17) TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL; KIZILTAS, M. V.; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞILPublication An HPLC Method for the Determination of Moxifloxacin in Breast Milk by Fluorimetric Detection with Precolumn Derivatization(2017-03-01) Tekkeli, S. E. Kepekci; GAZIOGLU, IŞIL; KIZILTAS, M. V.; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞILA new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 mu g mL(-1). Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 mu g mL(-1), respectively. Intra-day and interday repeatabilities were less than 3.15%.Publication Synthesis, antioxidant activity and SAR study of novel spiro-isatin-based Schiff bases(2018-12-01) Sönmez, Fatih; ZENGİN KURT, BELMA; Küçükislamoğlu, Mustafa; Avcı, Davut; GAZİOĞLU, IŞIL; Güneşli, Zuhal; ZENGİN KURT, BELMA; GAZİOĞLU, IŞILA new series of 21 Schiff bases of spiro-isatin was synthesized, and their DPPH, CUPRAC and ABTS cation radical scavenging abilities were investigated for antioxidant activity. The results showed that all the synthesized compounds exhibited antioxidant activity for each assay. 5 & x30d;-(2,3-Dihydroxybenzylideneamino)spiro[[1,3] dioxolane-2,3 & x30d;-indoline]-2 & x30d;-on (5c) (IC50 = 4.49 mu M, for DPPH; IC50 = 0.39 mu M, for ABTS(.+); and A(0.50) = 0.42 mu M, for CUPRAC) showed significantly better ABTS, CUPRAC and DPPH radical scavenging ability than quercetin (IC50 = 8.69 mu M, for DPPH; IC50 = 15.49 mu M, for ABTS(.+); and A(0.50) = 18.47 mu M, for CUPRAC), which is used as a standard. SAR study showed that the synthesized compounds had higher ABTS(.+) activity than DPPH and CUPRAC activities. Moreover, the compounds (5c and 5d), containing two hydroxyl groups, exhibited the highest antioxidant activities for all assays. Quantum chemical calculations were also carried out to support SAR results.Publication Investigation of the Anti-inflammatory Potential of Glucocapparin Isolated from Capparis ovata(2017-07-05) ÖZGÜN ACAR, ÖZDEN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI; ŞEN, ALAATTİN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIPublication Synthesis of novel Schiff bases as potential antioxidant agents(2018-06-30) SONMEZ, FATIH; Güneşli, Zuhal; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; KUCUKISLAMOGLU, MUSTAFA; ZENGİN KURT, BELMA; GAZİOĞLU, IŞILPublication In vitro biological screening of ten edible plants from middle black sea region(2015-11-27) ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; SEVGİ, ECE; Sonmez, Fatih; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; SEVGİ, ECEPublication Comparison of antioxidant, anticholinesterase, and antidiabetic activities of three curcuminoids isolated from Curcuma longa L.(2017-01-01) Kalaycıoğlu, Zeynep; Gazioglu, IŞIL; Erim, F. Bedia; GAZİOĞLU, IŞILAntioxidant, anticholinesterase and antidiabetic activities of three curcuminoids isolated from the Curcuma longa were simultaneously tested and compared in this study. The highest antioxidant power was detected for curcumin with the applied methods. The drug potentials of curcuminoids for Alzheimer-s disease were controlled. Bisdemethoxycurcumin (BDMC) showed substantial inhibitory activity. The activity of demethoxycurcumin (DMC) followed BDMC, whereas curcumin showed very little acetylcholinesterase inhibition activity. Antidiabetic activity of curcuminoids was evaluated by their -glucosidase inhibitory activities. All curcuminoids show activities with decreasing order as BDMC>curcumin>DMC. The significant activities of BDMC compared to its isomers and examination of chemical structures of isomers might be a starting point in designing new drugs for Alzheimer-s and Diabetes Mellitus.Publication High performance liquid chromatographic analysis of lercanidipine in human breast milk(2019-01-01) Tekkeli, Evrim Kepekci; GAZİOĞLU, IŞIL; TIRIS, GİZEM; Onal, Cem; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL; TIRIS, GİZEMA simple, rapid, precise and accurate isocratic reversed phase HPLC method was developed and validated for the determination of lercanidipine hydrochloride in pharmaceutical tablets and spiked human breast milk. The chromatographic separation was achieved on C18 (250x4.6 mmx5 mu m) column using a mobile phase consisting of acetonitrile and phospate buffer (pH=4) (55:45, v/v) at a flow rate of 1.1 mL/min and UV detection at 237 nm. The linearity of the proposed method was investigated in the range of 1.0-40 mu g/mL (r(2)=0.9990). The method was validated in terms of accuracy, precision, reproducibility, specificity, robustness, and detection and quantification limits, in accordance with ICH guidelines. The proposed method is found as suitable for routine quantification of lercanidipine in human breast milk.Publication Evaluation of Metabolites Produced by Fungal Biotransformation of Apigenin and Fisetin and Their Cytotoxic Activities(2020-03-01T00:00:00Z) GAZİOĞLU, IŞIL; Erdoğan, Oğuz; YANIKOĞLU, RABİA SARE; ÖZTÜRK, DİLEK; GAZİOĞLU, IŞIL; YANIKOĞLU, RABİA SARE; ÖZTÜRK CİVELEK, DİLEK