Person:
GAZİOĞLU, IŞIL

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Kurumdan Ayrılmıştır
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IŞIL
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GAZİOĞLU
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Now showing 1 - 10 of 68
  • PublicationMetadata only
    Effect of roasting on antioxidant and anticholinesterase capacities of coffee
    (2014-01-01) Yilmaz, Pelin Koseoglu; Hacibekiroglu, IŞIL; Kolak, Ufuk; GAZİOĞLU, IŞIL
    The aim of the study was to investigate the effect of the roasting degree on total phenolic and flavonoid contents, antioxidant and anticholinesterase activities of the hexane, acetone, ethanol and water extracts of coffee. The antioxidant capacity of the water extracts prepared according to the brewing procedure of Turkish coffee was measured for the first time by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and cupric ion reducing antioxidant capacity (CUPRAC) assays. The in vitro anticholinesterase activity of the coffee extracts and caffeine was determined for the first time. Water extracts of the coffee samples exhibited the highest 2,2--azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation radical scavenging, DPPH free radical scavenging and CUPRAC effects. The hexane extract of coffee roasted for 25 min showed the highest acetylcholinesterase inhibitory activity among the tested extracts. Caffeine, which is known to have therapeutic effect against Alzheimer-s disease, was found to possess almost the same anticholinesterase activity as galanthamine. The roasting degree had an irregular effect on the antioxidant and anticholinesterase activities of the coffee extracts. The caffeine contents in extracts of coffee roasted for 25 min, which had strong anticholinesterase effect, were determined by HPLC, and the water extract was found to possess the highest caffeine content.
  • PublicationOpen Access
    Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines
    (2020-01-01T00:00:00Z) GAZİOĞLU, IŞIL; Semen, Sevcan; Acar, Ozden Ozgun; KOLAK, Ufuk; Sen, Alaattin; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI
    Context: Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective: To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activities. Materials and methods: Buds, fruits, flowers, leaves and stems of C. ovata Desf. was dried, cut to pieces, then ground separately. From their dichloromethane/hexane (1:1) extracts, eight compounds were isolated and their structures were elucidated by NMR, mass spectroscopic techniques. The effects of compounds on the expression of inflammatory cytokines in SH-SY5Y cell lines were examined by qRT-PCR ranging from 4 to 96 mM. Cell viability was expressed as a percentage of the control, untreated cells. Results: This is a first report on isolation of triterpenoids and steroids from C. ovata with anti-inflammatory activity. One new triterpenoid ester olean-12-en-3b,28-diol, 3b-pentacosanoate (1) and two new natural steroids 5a,6a-epoxycholestan-3b-ol (5) and 5b,6b-epoxycholestan-3b-ol (6) were elucidated besides known compounds; oleanolic acid (2), ursolic acid (3), b-sitosterol (4), stigmast-5,22-dien-3b-myristate (7) and bismethyl-octylphthalate (8). mRNA expression levels as EC10 of all the tested seven genes were decreased, particularly CXCL9 (19.36-fold), CXCL10 (8.14-fold), and TNF (18.69) by the treatment of 26 mM of compound 1 on SH-SY5Y cells. Discussion and conclusions: Triterpenoids and steroids isolated from C. ovata were found to be moderate-strong anti-inflammatory compounds. Particularly, compounds 1 and 3 were found to be promising therapeutic agents in the treatment of inflammatory and autoimmune diseases.
  • PublicationOpen Access
    An HPLC Method for the Determination of Moxifloxacin in Breast Milk by Fluorimetric Detection with Precolumn Derivatization
    (2017-03-01) Tekkeli, S. E. Kepekci; GAZIOGLU, IŞIL; KIZILTAS, M. V.; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL
    A new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 mu g mL(-1). Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 mu g mL(-1), respectively. Intra-day and interday repeatabilities were less than 3.15%.
  • PublicationMetadata only
    Synthesis, antioxidant activity and SAR study of novel spiro-isatin-based Schiff bases
    (2018-12-01) Sönmez, Fatih; ZENGİN KURT, BELMA; Küçükislamoğlu, Mustafa; Avcı, Davut; GAZİOĞLU, IŞIL; Güneşli, Zuhal; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL
    A new series of 21 Schiff bases of spiro-isatin was synthesized, and their DPPH, CUPRAC and ABTS cation radical scavenging abilities were investigated for antioxidant activity. The results showed that all the synthesized compounds exhibited antioxidant activity for each assay. 5 & x30d;-(2,3-Dihydroxybenzylideneamino)spiro[[1,3] dioxolane-2,3 & x30d;-indoline]-2 & x30d;-on (5c) (IC50 = 4.49 mu M, for DPPH; IC50 = 0.39 mu M, for ABTS(.+); and A(0.50) = 0.42 mu M, for CUPRAC) showed significantly better ABTS, CUPRAC and DPPH radical scavenging ability than quercetin (IC50 = 8.69 mu M, for DPPH; IC50 = 15.49 mu M, for ABTS(.+); and A(0.50) = 18.47 mu M, for CUPRAC), which is used as a standard. SAR study showed that the synthesized compounds had higher ABTS(.+) activity than DPPH and CUPRAC activities. Moreover, the compounds (5c and 5d), containing two hydroxyl groups, exhibited the highest antioxidant activities for all assays. Quantum chemical calculations were also carried out to support SAR results.
  • PublicationMetadata only
    Biological activities and phenolic-flavonoid contents of bilberry extracts
    (2012-12-01T00:00:00Z) Hacibekiroǧlu, IŞIL; KOLAK, Ufuk; GAZİOĞLU, IŞIL
  • PublicationMetadata only
    Synthesis, anticholinesterase activity and molecular modeling study of novel carbamate-substituted thymol/carvacrol derivatives
    (2017-02-15) KURT, Belma Zengin; Gazioglu, IŞIL; Dag, AYDAN; Salmas, Ramin Ekhteiari; Kayik, Gulru; Durdagi, Serdar; Sonmez, Fatih; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; DAĞ, AYDAN
    New thymol and carvacrol derivatives with the carbamate moiety were synthesized and their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were evaluated. 5-isopropyl-2-methylphenyl(3-fluorophenyl)carbamate (29) was found to be the most potent AChE inhibitor with IC50 values of 2.22 mu M, and 5-isopropyl-2-methylphenyl (4-fluorophenyl)carbamate (30) exhibited the strongest inhibition against BuChE with IC50 value of 0.02 mu M. Additionally, the result of H4IIE hepatoma cell toxicity assay for compounds 18, 20, 29, 30 and 35 showed negligible cell death at 0.07-10 mu M. Moreover in order to better understand the inhibitory profiles of these molecules, molecular modeling studies were applied. Binding poses of studied compounds at the binding pockets of AChE and BuChE targets were determined. Predicted binding energies of these compounds as well as structural and dynamical profiles of molecules at the target sites were estimated using induced fit docking (IFD) algorithms and post-processing molecular dynamics (MD) simulations methods (i.e., Molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) approaches). (C) 2016 Elsevier Ltd. All rights reserved.
  • PublicationMetadata only
    Synthesis and Antibacterial Activity of New Sulfonamide Derivatives Containing Thiazolidine Ring
    (2020-03-01T00:00:00Z) Dandin, Gülnur; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL
  • PublicationMetadata only
    SIMULTANEOUS DETERMINATION OF FEBUXOSTAT AND MONTELUKAST IN HUMAN PLASMA BY USING FABRIC PHASE SORPTIVE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
    (2021-09-17T00:00:00Z) GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİM; Kabir, Abuzar; Aslan, Ceylin; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİM
  • PublicationMetadata only
    Evaluation of Metabolites Produced by Fungal Biotransformation of Apigenin and Fisetin and Their Cytotoxic Activities
    (2020-03-01T00:00:00Z) GAZİOĞLU, IŞIL; Erdoğan, Oğuz; YANIKOĞLU, RABİA SARE; ÖZTÜRK, DİLEK; GAZİOĞLU, IŞIL; YANIKOĞLU, RABİA SARE; ÖZTÜRK CİVELEK, DİLEK