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Effects of sitagliptin on ß-adrenoceptor mediated relaxation in streptozotocin-diabetic rat aorta

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dc.contributor.authorMuderrisoglu, Ayhanim Elif
dc.contributor.authorErdogan, Betul Rabia
dc.contributor.authorYeşilyurt, Zeynep Elif
dc.contributor.authorUyar Boztas, Ceren
dc.contributor.authorKaracomerlioglu, İrem
dc.contributor.authorAltan, Vecdi Melih
dc.contributor.authorArioglu İnan, Ebru
dc.contributor.institutionauthorALTAN, VECDİ MELİH
dc.date.accessioned2020-12-23T20:59:16Z
dc.date.available2020-12-23T20:59:16Z
dc.date.issued2020-11-13T00:00:00Z
dc.description.abstractBackground/aim: Dipeptidyl peptidase-4 (DPP4) inhibitors, a class of oral antidiabetic drugs, have been shown to be protective on the vascular system because of their antiinflammatory, antiatherosclerotic, and vasodilatory effects. ß2-adrenoceptors (ß2-ARs) mediate the vasorelaxation in the aorta. However, ß3-adrenoceptor-mediated relaxation has not been studied in diabetic aorta yet. Thus, we aimed to study the effect of sitagliptin treatment on ß2- and ß3-adrenoceptor-mediated relaxations in the diabetic rat aorta. Materials and methods: Eight-week old Sprague Dawley rats were divided into three groups: control, diabetic, sitagliptin treated diabetic. Diabetes was induced by injection of streptozotocin (35 or 40 mg/kg, intraperitoneally). After 10 weeks of diabetes, some of the diabetic rats were treated with sitagliptin (orally, 10mg/kg/day). ß2- and ß3-AR-mediated relaxation responses were evaluated by using isoprenaline and CL 316,243, respectively. ß3-AR-mediated relaxation experiments were repeated in presence of L-NAME. Western blotting and immunohistochemistry were performed to determine the abundance of ß3-adrenoceptor and endothelial nitric oxide synthase (eNOS). Results: The isoprenaline-mediated relaxation response was impaired in the diabetic group and sitagliptin treatment did not improve it. There was no significant change in CL316,243 mediated-relaxation or protein expression of ß3-ARs among the groups. However, the ratio of phosphorylated eNOS/NOS protein was increased markedly in the sitagliptin treated group, which points the stimulating effect of this drug towards the eNOS pathway. Conclusion: Our results indicate that sitagliptin treatment does not alter ß-AR-mediated relaxation in streptozotocin-diabetic rat aorta; however, it significantly stimulates the eNOS pathway. Future studies are needed to clarify the relationship between the eNOS pathway and DPP-4 inhibition.
dc.description.sponsorshipTürkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tubitak )
dc.description.sponsorshipTürkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tubitak )
dc.identifier.doi10.3906/sag-2007-234
dc.identifier.pubmed33185366
dc.identifier.urihttp://hdl.handle.net/20.500.12645/27807
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectaorta
dc.subjectendothelial nitric oxide synthase
dc.subjectsitagliptin
dc.subjectstreptozotocin induced diabetes
dc.subjectß-adrenoceptors
dc.subjectstreptozotocin induced diabetes
dc.titleEffects of sitagliptin on ß-adrenoceptor mediated relaxation in streptozotocin-diabetic rat aorta
dc.typeArticle
dspace.entity.typePublication
local.avesis.ide7dbfdc2-4ba3-4cd4-a32b-87ffce68aa6c
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
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relation.isAuthorOfPublication.latestForDiscoverye37c2bd0-23e0-4f76-9976-6414ab3c6ab9
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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