Publication: Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart
HATIRNAZ NG, Özden
Tansel, Cihan Demirci
Dogan, B. Sonmez Uydes
ARMUTAK, Elif İlkay
Background/Aim: This study was designed to provide further evidence for the interactions between hydrogen sulfide (H2S) and nitric oxide (NO) in ischemia/reperfiision (I/R) injury. Materials and Methods: Rat hearts were studied with the Langendorff technique using the H2S donor sodium hydrosulfide (NaHS, 40 mu M) and the cystathionine gamma-lyase (CTH or CSE) inhibitor DL-propargylglycine (PAG, 1 mM). NO synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME, 30 mg/kg, 7 days) was administered before the isolation. The hearts were homogenized for biochemical and molecular analysis. Results: NaHS reversed I/R-induced cardiac performance impairment, increased tissue nitric oxide production and decreased tissue markers for cardiac injury, while L-NAME inhibited these effects. The expression of CTH was increased with PAG, which was suppressed by L-NAME. Conclusion: H2S and NO increase each other-s production suggesting their interaction and cooperation in cardioprotection against I/R injury.
Hydrogen Sulphide , Isolated Rat Heart , Nitric Oxide , Ischemia , Reperfusion Injury
ÜSTÜNOVA S., TAKIR S., YILMAZER N., Bulut H., ALTINDİREK D., HATIRNAZ NG Ö., Tansel C. D. , Dogan B. S. U. , ÖZBEK U., ARMUTAK E. İ. , et al., -Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart-, IN VIVO, cilt.34, ss.2507-2516, 2020