Person: TOPÇU, GÜLAÇTI
40 results
Search Results
Now showing 1 - 10 of 40
Publication Open Access Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines(2020-01-01T00:00:00Z) GAZİOĞLU, IŞIL; Semen, Sevcan; Acar, Ozden Ozgun; KOLAK, Ufuk; Sen, Alaattin; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIContext: Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective: To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activities. Materials and methods: Buds, fruits, flowers, leaves and stems of C. ovata Desf. was dried, cut to pieces, then ground separately. From their dichloromethane/hexane (1:1) extracts, eight compounds were isolated and their structures were elucidated by NMR, mass spectroscopic techniques. The effects of compounds on the expression of inflammatory cytokines in SH-SY5Y cell lines were examined by qRT-PCR ranging from 4 to 96 mM. Cell viability was expressed as a percentage of the control, untreated cells. Results: This is a first report on isolation of triterpenoids and steroids from C. ovata with anti-inflammatory activity. One new triterpenoid ester olean-12-en-3b,28-diol, 3b-pentacosanoate (1) and two new natural steroids 5a,6a-epoxycholestan-3b-ol (5) and 5b,6b-epoxycholestan-3b-ol (6) were elucidated besides known compounds; oleanolic acid (2), ursolic acid (3), b-sitosterol (4), stigmast-5,22-dien-3b-myristate (7) and bismethyl-octylphthalate (8). mRNA expression levels as EC10 of all the tested seven genes were decreased, particularly CXCL9 (19.36-fold), CXCL10 (8.14-fold), and TNF (18.69) by the treatment of 26 mM of compound 1 on SH-SY5Y cells. Discussion and conclusions: Triterpenoids and steroids isolated from C. ovata were found to be moderate-strong anti-inflammatory compounds. Particularly, compounds 1 and 3 were found to be promising therapeutic agents in the treatment of inflammatory and autoimmune diseases.Publication Metadata only Preclinical studies evaluating the therapeutic efficacy of novel natural compound olean-12-en-28-ol, 3β pentacosanoate for multiple sclerosis(2022-09-26) Şenol H.; Özgün-Acar Ö.; Dağ A.; Eken A.; Acar B.; Aktaş Pepe N.; Güner H.; Aykut Z. G.; Çevik Kaplan S.; Ayaz Güner Ş.; et al.; ŞENOL, HALIL; DAĞ, AYDAN; TOPÇU, GÜLAÇTIPublication Metadata only Investigation of cytotoxic and apoptotic effects of 63 compounds obtained from Salvia species: Promising anticancer agents(2022-05-01T00:00:00Z) Kandemir, Sevgi Irtegun; FİDAN, HİLAL SARUHAN; Yener, Ismail; METE, NURİYE; ERTAŞ, ABDULSELAM; TOPÇU, GÜLAÇTI; KOLAK, Ufuk; TOPÇU, GÜLAÇTISince ancient time, Salvia L. species have been commonly used to treat colds, bronchitis, tuberculosis, heart diseases, and menstrual and digestive disorders in traditional medicine all around the world. They have been also used as tea and spice. Studies indicated that diterpenes and triterpenes isolated from Salvia species possess various pharmacological and biological effects such as anti-inflammatory, antiviral, cytotoxic, antioxidant, and hepatotoxic activities. Flavones were also shown to have antimicrobial, antioxidant, and cytotoxic potentials. Salvia extracts also exhibit anti-Alzheimer, antiseptic, cardiovascular, antihypertensive, and antituberculous effects. To investigate the effects of 63 secondary metabolites from Salvia species on cell viability and apoptosis, Salvia secondary metabolites including 25 phenolics, 4 fatty acids, 19 abietane diterpenoids, 12 triterpenoids, and three steroids were examined on healthy cell line (PDF), breast cancer (MCF-7), and colon cancer (HT-29) cell lines using MTT method. In addition, the effects of rosmarinic acid, 6,7-dehydroroyleanone, acetyl royleanone, ferruginol, carnosic acid, carnosol, cryptotanshinone, beta-sitosterol, and ursolic acid on pro-apoptotic Bax and antiapoptotic Bcl-2 protein expression levels were investigated by Western Blot method. Practical applications Phenolic compounds (apigenin, chrysin, and luteolin) and diterpenes (especially dihydrotanshinone I, carnosic acid, and carnosol), and almost all of the triterpenes exhibited high toxic effects on healthy cell line. Cytotoxic effects of cryptotanshinone, 12-hydroxy abieta-1,3,5(10),8,11,13-hexaene, 12-demethylmulticauline, 6,7-dehydroroyleanone, acetyl royleanone, ferruginol, ursolic acid, and 3-acetyl lupeol were relatively higher than their toxic effects. Acetyl royleanone, 6,7-dehydroroyleanone, carnosic acid, and cryptotanshinone were found to have anticancer potential based on their modulating effects on the expression levels of Bax and Bcl-2 proteins which play important roles in the regulation of apoptosis. The results of the present study showed that acetyl royleanone, cryptotanshinone, 6,7-dehydroroyleanone, carnosic acid, and cryptotanshinone have potential to be used in the pharmaceutical industry.Publication Metadata only Importance of Functional Foods in Brain Health(2023-03-19) Topçu G.; TOPÇU, GÜLAÇTIPublication Metadata only Di-, and Triterpenoids Isolation and LC-MS Analysis of Salvia marashica Extracts with Bioactivity Studies(2021-11-01T00:00:00Z) Toraman, Gulbahar O. Alim; Aydin, Sibel Kiran; Ertas, Abdulselam; Boga, Mehmet; EROL, EBRU; Saygi, Tuba Kusman; Halfon, Belkis; TOPÇU, GÜLAÇTI; EROL, EBRU; ALİM TORAMAN, GÜLBAHAR ÖZGE; TOPÇU, GÜLAÇTIIn this study, dichloromethane, acetone, and methanol extracts of the aerial parts of the Salvia marashica plant which is an endemic species to Anatolia, were investigated. The total phenolic amounts of these extracts were determined as pyrocatechol equivalent and total flavonoids as quercetin equivalent. Antioxidant activity was determined by four complementary methods including inhibition of lipid peroxidation (by beta-carotene color expression), DPPH free radical scavenging activity, ABTS cation radical scavenging activity and CUPRAC methods. Anticholinesterase activity of the extracts was investigated by the Ellman method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Viability and cytotoxic activity tests were carried out on the fibroblast L929 cells and cytotoxic A549 lung cancer cells, respectively. The triterpenoids and diterpenoids constitute the major secondary metabolites of the S. marashica acetone and methanol extracts isolated by chromatographic methods. Their structures were determined based on spectroscopic methods, namely NMR and mass analyses. Ten terpenoids were obtained from either acetone or methanol extracts of the S. marashica. Seven of them were triterpenoids, elucidated as lupeol, lupeol-3-acetate, lup-12, 20(29)-diene, lup-20(29)-ene, alpha-amyrin-tetracosanoate, oleanolic acid and ursolic acid besides a steroid beta-sitosterol. Two abietane diterpenes, abieta-8,11,13-triene (1) and 18-acetoxymethylene-abieta-8,11,13-triene (2), were obtained from the acetone extract which were isolated from a Salvia species for the first time in the present study. The methanol extract was found to be very rich in rosmarinic acid determined by LC-MS/MS analysis.Publication Metadata only Yeşil Alg Caulerpa cylindracea Sonder-den İzole Edilen Bis-İndol Alkaloidlerin SARS-CoV-2-ye Karşı in vitro Antiviral Aktivitesi(2022-06-23T00:00:00Z) Erol, Ebru; Avşar, Timuçin; Okudan, Emine Şükran; Topçu, Gülaçtı; EROL, EBRU; ALİM TORAMAN, GÜLBAHAR ÖZGE; TOPÇU, GÜLAÇTICaulerpaceae familyasına ait olanCaulerpa cylindraceaSonder yeşil bir deniz yosunudur. Gelibolu yarımadası kıyılarından SCUBA dalışlatoplananCaulerpacylindracea-nın builk kimyasal araştırması ile caulerpin1, mono metil caulerpinat2, beta-sitosterol ve palmitik asit izole edildi. İzole edilen bileşiklerin kimyasal yapıları, 1D- ve 2D-NMR ve kütle spektroskopik analizlerle aydınlatıldı. Caulerpin ve mono metil caulerpinatınin vitroanti-viral aktivitesi, Spike proteini ve main proteaza (3CL) karşı incelendi. Bu çalışmanın bulguları, caulerpin ve mono metil caulerpinatın Spike ve ACE2 etkileşimini önemli ölçüde inhibe ettiğini ortaya koymaktadır. Ayrıca, izole edilen bileşiklerin sitotoksik aktivitesi, sağlıklı fibroblast hücre hattı CCD-1079Sk ve iki meme kanseri hücre hattı; MDA-MB-231 ve MCF-7’ye karşı incelendi.Publication Metadata only Chemical composition and biological activities of Cypriot propolis(2021-09-01T00:00:00Z) NALBANTSOY, AYŞE; SARIKAHYA, NAZLI; Ozverel, Cenk Serhan; Barlas, Ayse Bercin; Kirci, Damla; AKGÜN, İSMAİL HAKKI; Yalcin, Tansel; Duven, Gamze; KIŞLA, DUYGU; DEMİRCİ, BETÜL; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHANPropolis compositions are highly variable, depending on the geographic region and the season of collection. In this study, propolis samples from seven different regions of Cyprus were studied for the first time by means of chemical content and biological activities. Secondary metabolite composition was determined by LC-HRMS. While the major flavonoids found were isosakuranetin, naringenin, rhamnocitrin, diosmetin, chrysin and acacetin, interestingly verbascoside, a phenylethanoid glycoside, and chlorogenic acid were identified as the major compounds in the ethanol-water extracts. alpha-Pinene was detected as the major compound of propolis extracts according to the volatile compositions via GC-MS. Karaoglanoglu and Tirmen extracts, presenting different chemical profiles, exerted enormous cytotoxic activity by MTT assay (IC50: 2.36-11.56 mu g/mL; 1.44-9.33 mu g/mL, respectively). The highest iNOS inhibition potential was detected in the Karpaz extract (IC50:2.6 mu g/mL) in LPS induced RAW 264.7 cells whereas the Guzelyurt sample demonstrated remarkable antioxidant (88.82 +/- 0.10%) and antimicrobial activities (with a MIC value of 31.2 mu g/mL against S. aureus, S. epidermidis, E. faecium, and E. faecalis).Publication Metadata only Synthesis and Comprehensive in Vivo Activity Profiling of Olean-12-en-28-ol, 3β-Pentacosanoate in Experimental Autoimmune Encephalomyelitis: A Natural Remyelinating and Anti-Inflammatory Agent(2023-01-01) Şenol H.; Özgün Acar Ö.; Dağ A.; Eken A.; Güner H.; Aykut Z. G.; Topçu G.; Şen A.; ŞENOL, HALIL; DAĞ, AYDAN; TOPÇU, GÜLAÇTIPublication Open Access Special issue dedicated to the memory of Professor Ayhan Ulubelen (1931-2020)(2021-01-01T00:00:00Z) TOPÇU, GÜLAÇTI; TOPÇU, GÜLAÇTIPublication Metadata only Centaurea nemecii NAB. Ekstrelerinin Antikolinesteraz İnhibitör Aktivitesi(2021-12-08T00:00:00Z) Alim Toraman, Gülbahar Özge; Kuşman Sayğı, Tuba; Bütün, Burcu; Topçu, Gülaçtı; ALİM TORAMAN, GÜLBAHAR ÖZGE; TOPÇU, GÜLAÇTI