Sıçanlarda siyatik sinirin ezilme yaralanması sonrası yüksek yoğunluklu lazer biyostimülasyon tedavisinin rejeneratif etkilerinin değerlendirilmesi
In complications that may occur as a result of surgical procedures performed in the maxillofacial region; Neurosensory disorders such as loss of function in nerves, paresthesia, hypoesthesia, neuropathic pain can be observed due to peripheral or central nervous system injury. The time of initiation of treatment varies according to the type of damage and the area where it is located. Invasive, noninvasive and conventional treatments are applied as treatment methods. Although there are studies showing the regenerative effect of the nerve by applying many treatment methods after degenerative and traumatic injury of the peripheral nervous system, there is no study in the literature on high-intensity laser therapy. The left sciatic nerves of all rats were crushed with a surgical clamp for 30 s to create nerve damage. Following the nerve crush injuries of sciatic nerve, thirty-three rats were randomly divided into three groups as control, Low-level laser theraphy (LLLT), and HILT groups. The injured sciatic nerve of rats in the control group were left to heal spontaneously, whereas HILT (120J/session and wavelength 1024 nm) and LLLT (2.4J/session and 650 nm) were started immediately after surgery and performed once every 3 days during the postoperative period. Regeneration was investigated by sciatic functional index (SFI), electrophysiological evaluations and histomorphometric evaluations. On the 23th day of the healing period the HILT group presented better SFI results when compared with control group(p=,012) whereas there was no statisticaly significant differences between HILT-LLLT (p=,473) and LLLT-control(p=,270) groups. At the end of 30 days healing period significanlty better SFI scores were noted in the HILT group compared with LLLT and control groups (p=,002, p=,000) whereas there was no statistically significant differences between the LLLT and control groups(p=,419). There was no statisticaly significant differences between three groups regarding the amplitude values of the electrophysiological evaluations. Latency and duration values revealed that HILT group has statisticaly significant differences between LLLT(p=,002, p=,014) and control(p=,003, p=,000) groups that was no statisticaly differences between LLLT and control groups (p=1.000, p=,162). Histological evaluations revealed that LLLT group has statisticaly higher schwann cell numbers compared to HILT (p=,048) and control (p=,003) groups whereas there was no statisticaly significant differences between HILT and control groups (p=,59). G-ratio evaluation revealed that, the number of nerve fibers with ranges of 0,55–0.69 has higher in the HILT group than other groups. According to functional, histomorphometric and electrophysiological investigations HILT revealed better results than LLLT on peripheral nerve regeneration after crush injury. HILT seems to be supperior than LLLT regarding to higher penetration depth and efficacy during peripheral nerve regeneration.