Effect of Er:YAG laser enamel conditioning and moisture on the microleakage of a hydrophilic sealant
Abstract
For a given sealant, successful pit and fssure sealing is principally governed by the enamel conditioning technique and the
presence of moisture contamination. A new generation of hydrophilic resin sealants is reported to tolerate moisture. This
study investigates the impact of Er:YAG laser pre-conditioning and moisture contamination on the microleakage of a recent
hydrophilic sealant. Occlusal surfaces of extracted human molars were either acid etched (n = 30), or successively lased and
acid etched (n = 30). Ten teeth from each group were either air-dried, water-contaminated, or saliva-contaminated prior to
sealing with UltraSeal XT® hydro™. Samples were inspected for penetration of fuchsin dye following 3000 thermocycles
between 5 and 50 °C, and the enamel–sealant interfaces were observed by scanning electron microscopy (SEM). Signifcant
diferences in microleakage were evaluated using the Mann–Whitney U test with Bonferroni adjustment (p = 0.05). Laser
pre-conditioning signifcantly reduced dye penetration irrespective of whether the enamel surface was moist or dry. Microleakage of water-contaminated acid etched teeth was signifcantly greater than that of their air-dried or saliva-contaminated
counterparts. SEM analysis demonstrated good adaptation in all groups with the exception of water-contaminated acid etched
teeth which exhibited relatively wide gaps. In conclusion, this hydrophilic sealant tolerates the presence of saliva, although
water was found to impair its sealing ability. Laser pre-conditioning signifcantly decreases microleakage in all cases.
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