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Gold nanoconjugates reinforce the potency of conjugated cisplatin and doxorubicin

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dc.contributor.authorIram, Sana
dc.contributor.authorZahera, Manaal
dc.contributor.authorKhan, Salman
dc.contributor.authorKhan, IMRAN
dc.contributor.authorSyed, Asad
dc.contributor.authorAnsary, Abu Ayoobul
dc.contributor.authorAmeen, Fuad
dc.contributor.authorShair, Omar H. M.
dc.contributor.authorKhan, Mohd Sajid
dc.contributor.institutionauthorKHAN, IMRAN
dc.date.accessioned2021-04-10T20:59:14Z
dc.date.available2021-04-10T20:59:14Z
dc.date.issued2017-12-01T00:00:00Z
dc.description.abstractOsteosarcoma or osteogenic sarcoma is the most common and prevalent cancerous tumor of bone and occurs especially in children and teens. Recent treatment strategy includes a combination of both chemotherapy and surgeries. Although, the use of single drug-based chemotherapy treatment remains unsatisfactory. Therefore, combinatorial therapy has emerged as a potential strategy for treatment with limited side- effects. Here, we evaluated the combinatorial anticancerous effect of cisplatin (CIS) and doxorubicin (DOX) bioconjugated bromelain encapsulated gold nanoparticles (B-AuNPs conjugated CIS and DOX) in the treatment of osteosarcoma. The synthesized B-AuNPs conjugated CIS and DOX were characterized by various characterization techniques like UV-vis spectroscopy, TEM, DLS and zeta potential to ensure the synthesis, size, shape, size distribution and stability. Drug loading efficiency bioconjugation of CIS and DOX was ensured by UV-vis spectroscopy. Bioconjugation of CIS and DOX was further confirmed using UV-vis spectroscopy, TEM, DLS, Zeta potential and FT-IR analysis. The combinatorial effect of CIS and DOX in B-AuNPs conjugated CIS and DOX showed highly improved potency against MG-63 and Saos-2 cells at a very low concentration where primary osteoblasts didn-t show any cytotoxic effect. The apoptotic effect of B-AuNPs conjugated CIS and DOX on osteosarcoma and primary osteoblasts cells were analyzed by increased permeability of the cell membrane, condensed chromatin and deep blue fluorescent condensed nucleus. The results clearly showed that B-AuNPs conjugated CIS and DOX significantly improved the potency of both the chemotherapeutic drugs by delivering them specifically into the nucleus of cancer cells through caveolae-dependent endocytosis. Thus, the greater inhibitory effect of combinatorial drugs (B-AuNPs conjugated CIS and DOX) over single drug based chemotherapy would be of great advantage during osteosarcoma treatment. (C) 2017 Published by Elsevier B.V.
dc.identifier.citationIram S., Zahera M., Khan S., Khan I., Syed A., Ansary A. A. , Ameen F., Shair O. H. M. , Khan M. S. , -Gold nanoconjugates reinforce the potency of conjugated cisplatin and doxorubicin-, COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.160, ss.254-264, 2017
dc.identifier.doi10.1016/j.colsurfb.2017.09.017
dc.identifier.scopus85029587205
dc.identifier.urihttp://hdl.handle.net/20.500.12645/28728
dc.identifier.wosWOS:000418978500031
dc.titleGold nanoconjugates reinforce the potency of conjugated cisplatin and doxorubicin
dc.typeArticle
dspace.entity.typePublication
local.avesis.ide7a14687-0620-4103-a2b9-4907d2da675c
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublication096be806-cf26-4766-b5b4-26f30c593af2
relation.isAuthorOfPublication.latestForDiscovery096be806-cf26-4766-b5b4-26f30c593af2
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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