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Evaluation of Catechin Synergistic and Antibacterial Efficacy on Biofilm Formation and acrA Gene Expression of Uropathogenic E. coli Clinical Isolates

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dc.contributor.authorJubair, Najwan
dc.contributor.authorMogana, R.
dc.contributor.authorFATIMA, AYESHA
dc.contributor.authorMahdi, Yasir K.
dc.contributor.authorAbdullah, Nor Hayati
dc.contributor.institutionauthorFATIMA, AYESHA
dc.date.accessioned2022-10-04T20:59:11Z
dc.date.available2022-10-04T20:59:11Z
dc.date.issued2022-09-01T00:00:00Z
dc.description.abstractUropathogenic Escherichia coli has a propensity to build biofilms to resist host defense and antimicrobials. Recurrent urinary tract infection (UTI) caused by multidrug-resistant, biofilm-forming E. coli is a significant public health problem. Consequently, searching for alternative medications has become essential. This study was undertaken to investigate the antibacterial, synergistic, and antibiofilm activities of catechin isolated from Canarium patentinervium Miq. against three E. coli ATCC reference strains (ATCC 25922, ATCC 8739, and ATCC 43895) and fifteen clinical isolates collected from UTI patients in Baghdad, Iraq. In addition, the expression of the biofilm-related gene, acrA, was evaluated with and without catechin treatment. Molecular docking was performed to evaluate the binding mode between catechin and the target protein using Autodock Vina 1.2.0 software. Catechin demonstrated significant bactericidal activity with a minimum inhibitory concentration (MIC) range of 1-2 mg/mL and a minimum bactericidal concentration (MBC) range of 2-4 mg/mL and strong synergy when combined with tetracycline at the MBC value. In addition, catechin substantially reduced E. coli biofilm by downregulating the acrA gene with a reduction percent >= 60%. In silico analysis revealed that catechin bound with high affinity ( increment G = -8.2 kcal/mol) to AcrB protein (PDB-ID: 5ENT), one of the key AcrAB-TolC efflux pump proteins suggesting that catechin might inhibit the acrA gene indirectly by docking at the active site of AcrB protein.
dc.identifier.citationJubair N., Mogana R., FATIMA A., Mahdi Y. K. , Abdullah N. H. , -Evaluation of Catechin Synergistic and Antibacterial Efficacy on Biofilm Formation and acrA Gene Expression of Uropathogenic E. coli Clinical Isolates-, ANTIBIOTICS-BASEL, cilt.11, sa.9, 2022
dc.identifier.doi10.3390/antibiotics11091223
dc.identifier.pubmed36140002
dc.identifier.scopus85138511230
dc.identifier.urihttp://hdl.handle.net/20.500.12645/31056
dc.identifier.wosWOS:000856180600001
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcrAB-TolC efflux pump
dc.subjectAutodock Vina
dc.subjectCanarium patentinervium Miq.
dc.subjectantibiofilm activity
dc.subjectmolecular docking
dc.subjectmultidrug resistance
dc.titleEvaluation of Catechin Synergistic and Antibacterial Efficacy on Biofilm Formation and acrA Gene Expression of Uropathogenic E. coli Clinical Isolates
dc.typeArticle
dspace.entity.typePublication
local.avesis.ide3009d26-e1a6-478e-8df1-44a35f8b5bc3
local.publication.isinternational1
relation.isAuthorOfPublication42c43eb9-9434-445b-a4b1-9aedfd0731a6
relation.isAuthorOfPublication.latestForDiscovery42c43eb9-9434-445b-a4b1-9aedfd0731a6
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