Publication:
Determination of Early Diagnostic Biomarkers of Renal Dysfunction After Cardiopulmonary Bypass: miR-21 and miR-10a Mediated Postoperative Inflammation

dc.contributor.authorCagil F. Z.
dc.contributor.authorKoray A.
dc.contributor.authorRAFIQI A. M.
dc.contributor.authorSaracoglu A.
dc.contributor.authorOter G. N.
dc.contributor.authorTetik S.
dc.contributor.institutionauthorRAFIQI, ABDUL MATTEEN
dc.date.accessioned2024-05-14T21:50:13Z
dc.date.available2024-05-14T21:50:13Z
dc.date.issued2024-03-01
dc.description.abstractObjective: Acute renal failure (ARF) prevalence is high among patients who undergo cardiopulmonary bypass (CPB), and this condition can only be diagnosed via serum creatinine level (sCr) conventionally within 48 hours. Therefore, we need early novel diagnosis biomarkers to start preventive treatment of ARF. For that reason, we aimed to analyze if plasma miR-21 derived from heart, correlates with kidney- enriched miR-10a during inflammatory IL-6, IL-1 beta, and TNF-alpha response in terms of acute renal failure 30 minutes after CPB. Methods: Patients (n=46, Female:8 and Male:38), aged 61.08 +/- 9.41, who underwent CPB surgery were included. Blood samples were collected during the pre - and post-CPB (30 minutes after CPB). Demographic data of all cases were collected. Quantification of expression levels of miR-21 and miR-10a was done via quantitative PCR (qPCR). Determination of plasma concentration of relevant cytokines, IL-6, IL-1 beta, and TNF-alpha was done via ELISA. Results: The circulating level of miR-21 during post-CPB period (-11.78 +/- 6.98) was significantly higher (p 0.05) in the circulating level of miR-10a between pre - (-12.22 +/- 3.55) and post-CPB (-11.60 +/- 3.36) periods. When we compared the mean Delta Delta Ct values of miR-21 and miR-10a, downregulation was observed in the expression level of miR-10a (0.62 +/- 3.77) whilst the expression level of miR-21 (-5.22 +/- 7.25) was upregulated (p Conclusion: Prospectively, these data suggests that high miR-21 levels is a promising indicator and can be a candidate as an early novel biomarker for diagnosis of acute renal failure 30 minutes after CPB.
dc.identifier.citationCagil F. Z., Koray A., RAFIQI A. M., Saracoglu A., Oter G. N., Tetik S., "Determination of Early Diagnostic Biomarkers of Renal Dysfunction After Cardiopulmonary Bypass: miR-21 and miR-10a Mediated Postoperative Inflammation", CLINICAL AND EXPERIMENTAL HEALTH SCIENCES, sa.1, ss.245-252, 2024
dc.identifier.doi10.33808/clinexphealthsci.1222499
dc.identifier.endpage252
dc.identifier.issn2459-1459
dc.identifier.issue1
dc.identifier.startpage245
dc.identifier.urihttps://avesis.bezmialem.edu.tr/api/publication/27f192b1-412f-4a18-832b-ff9db5c663d5/file
dc.identifier.urihttps://hdl.handle.net/20.500.12645/39275
dc.identifier.wosWOS:001199753500001
dc.relation.ispartofCLINICAL AND EXPERIMENTAL HEALTH SCIENCES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectDahili Tıp Bilimleri
dc.subjectTıbbi Ekoloji ve Hidroklimatoloji
dc.subjectSağlık Bilimleri
dc.subjectMedicine
dc.subjectInternal Medicine Sciences
dc.subjectMedical Ecology and Hydroclimatology
dc.subjectHealth Sciences
dc.subjectTIP, ARAŞTIRMA VE DENEYSEL
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectMEDICINE, RESEARCH & EXPERIMENTAL
dc.subjectCLINICAL MEDICINE
dc.subjectClinical Medicine (MED)
dc.subjectAraştırma ve Teori
dc.subjectİncelemeler ve Referanslar (tıbbi)
dc.subjectResearch and Theory
dc.subjectReviews and References (medical)
dc.titleDetermination of Early Diagnostic Biomarkers of Renal Dysfunction After Cardiopulmonary Bypass: miR-21 and miR-10a Mediated Postoperative Inflammation
dc.typeArticle
dspace.entity.typePublication
local.avesis.id27f192b1-412f-4a18-832b-ff9db5c663d5
relation.isAuthorOfPublicationdd3a305d-7fb4-4793-843e-88b3e0e64452
relation.isAuthorOfPublication.latestForDiscoverydd3a305d-7fb4-4793-843e-88b3e0e64452
Files