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Effect of priming on thymoquinone content and in vitro plant regeneration with tissue culture of black cumin (Nigella sativa L.) seeds

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dc.contributor.authorRezaei, Fereshteh
dc.contributor.authorIsik, Selin
dc.contributor.authorKARTAL, MURAT
dc.contributor.authorErdem, Sinem Aslan
dc.contributor.institutionauthorKARTAL, MURAT
dc.date.accessioned2020-01-13T20:59:24Z
dc.date.available2020-01-13T20:59:24Z
dc.date.issued2018-07-01T00:00:00Z
dc.description.abstractThe aim of this study is to analyze the changes on thymoquinone content, which is the major constituent of black cumin (Nigella sativa L.) seeds, by using priming methods. For the priming studies, seeds placed in distilled water (18 - 20 hours), PEG (Polyethylene glycol 10%, 20%) and mannitol (4% and 6%) for 20 hours and subsequently dried for 24 hours on blotting paper at room temperature (24 degrees C +/- 1 degrees C). The best priming results were obtained in the 4% of mannitol treatment. Control (not primed) Nigella seeds and 4% of mannitol primed seeds were planted in both greenhouse and farm conditions. Seed extracts were analyzed with HPLC to compare the amount of thymoquinone. The results reveal that seed priming with 4% of mannitol increases the amount of thymoquinone in Nigella sativa. In addition, the effects of plant growth hormones on callus regeneration of these primed seeds were investigated herein. (C) 2018 ACG Publications. All rights reserved.
dc.identifier.citationRezaei F., Isik S., KARTAL M., Erdem S. A. , -Effect of priming on thymoquinone content and in vitro plant regeneration with tissue culture of black cumin (Nigella sativa L.) seeds-, JOURNAL OF CHEMICAL METROLOGY, cilt.12, ss.89-98, 2018
dc.identifier.doi10.25135/jcm.18.18.09.950
dc.identifier.urihttps://openaccess.bezmialem.edu.tr/handle/20.500.12645/12276
dc.identifier.wosWOS:000454236700001
dc.titleEffect of priming on thymoquinone content and in vitro plant regeneration with tissue culture of black cumin (Nigella sativa L.) seeds
dc.typeArticle
dspace.entity.typePublication
local.avesis.id7fe4b05f-3b30-485d-9703-65fa7b6e54bd
local.publication.goal02 - Açlığa Son
local.publication.isinternational1
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relation.isGoalOfPublication.latestForDiscovery5246edf8-60d4-4a7b-9a27-c73e4a636141
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