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Melatonin attenuates phenytoin sodium-induced DNA damage

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dc.contributor.authorErenberk, UFUK
dc.contributor.authorDUNDAROZ, Rusen
dc.contributor.authorGOK, Ozlem
dc.contributor.authorUysal, Omer
dc.contributor.authorAGUS, Sami
dc.contributor.authorYuksel, Adnan
dc.contributor.authorYilmaz, Bayram
dc.contributor.authorKILIC, Ulkan
dc.contributor.institutionauthorERENBERK, UFUK
dc.contributor.institutionauthorUYSAL, ÖMER
dc.date.accessioned2019-10-05T21:51:55Z
dc.date.available2019-10-05T21:51:55Z
dc.date.issued2014-04-01
dc.description.abstractPhenytoin sodium (PHT Na+) is a potent antiepileptic drug against epileptic seizures and is used as a prophylactic treatment in traumatic brain injury. PHT Na+ leads to the formation of reactive oxygen species (ROS), and DNA is a crucial molecular target of ROS-initiated toxicity. Melatonin and its metabolites possess free-radical-scavenging activity. We therefore designed this study to investigate the potential protective effect of melatonin against PHT Na+-induced DNA damage by using the comet assay in a rat model in vivo. Thirty-three 3-month-old male Wistar rats were divided into five groups of control treated with isotonic sodium chloride (a single injection of isotonic sodium chloride and 100 mL in drinking water for 10 days), ethanol treated (in drinking water for 10 days containing 100 mL of ethanol in each 300-mL drinking bottle), melatonin treated (4 mg/kg body weight [b.w.] intraperitoneally [i.p.] at the start, in drinking water for 10 days), PHT Na+ treated (a single i.p. injection of 50 mg/kg) and PHT Na+ (50 mg/kg b.w., single i.p.) and melatonin (4 mg/kg b.w. i.p. at the start and 4 mg/kg in drinking water for 10 days) cotreated. To determine the protective effects of melatonin, the comet assay was performed using lymphocytes isolated in different time intervals (0, 15, 30, 45 and 60 minutes) from each group of animals. On days 1, 3, 7 and 10, blood samples were taken and the comet assay technique was performed. Our present data suggest that melatonin reversed PHT Na+-induced DNA damage.
dc.description.sponsorshipTürkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tubitak )
dc.identifier
dc.identifier.citationErenberk U., DUNDAROZ R., GOK O., Uysal O., AGUS S., Yuksel A., Yilmaz B., KILIC U., -Melatonin attenuates phenytoin sodium-induced DNA damage-, DRUG AND CHEMICAL TOXICOLOGY, cilt.37, ss.233-239, 2014
dc.identifier.doi10.3109/01480545.2013.838777
dc.identifier.scopus84896695392
dc.identifier.urihttps://hdl.handle.net/20.500.12645/8310
dc.identifier.wosWOS:000332034900016
dc.language.isoen
dc.titleMelatonin attenuates phenytoin sodium-induced DNA damage
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameTprecd
local.avesis.idcee23a84-4dcf-4973-8aa7-c3e3e413cddf
local.avesis.response8186
local.publication.isinternational1
relation.isAuthorOfPublication4a4f159b-42a7-4200-9172-62491b378e88
relation.isAuthorOfPublication19df24c5-c3e8-4158-8eed-717f885c608d
relation.isAuthorOfPublication.latestForDiscovery19df24c5-c3e8-4158-8eed-717f885c608d
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