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Development of an HPLC-UV Method for the Analysis of Drugs Used for Combined Hypertension Therapy in Pharmaceutical Preparations and Human Plasma

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dc.contributor.authorTekkeli, Serife Evrim Kepekci
dc.contributor.institutionauthorTEKKELİ, ŞERİFE EVRİM
dc.date.accessioned2019-10-05T14:47:23Z
dc.date.available2019-10-05T14:47:23Z
dc.date.issued2013-01-01
dc.description.abstractA simple, rapid, and selective HPLC-UV method was developed for the determination of antihypertensive drug substances: amlodipine besilat (AML), olmesartan medoxomil (OLM), valsartan (VAL), and hydrochlorothiazide (HCT) in pharmaceuticals and plasma. These substances are mostly used as combinations. The combinations are found in various forms, especially in current pharmaceuticals as threesome components: OLM, AML, and HCT (combination I) and AML, VAL, and HCT (combination II). The separation was achieved by using an RP-CN column, and acetonitrile-methanol-10 mmol orthophosphoric acid pH 2.5 (7 : 13 : 80, v/v/v) was used as a mobile phase; the detector wavelength was set at 235 nm. The linear ranges were found as 0.1-18.5 μ g/mL, 0.4-25.6 μ g/mL, 0.3-15.5 μ g/mL, and 0.3-22 μ g/mL for AML, OLM, VAL, and HCT, respectively. In order to check the selectivity of the method for pharmaceutical preparations, forced degradation studies were carried out. According to the validation studies, the developed method was found to be reproducible and accurate as shown by RSD ≤6.1%, 5.7%, 6.9%, and 4.6% and relative mean error (RME) ≤10.6%, 5.8%, 6.5%, and 6.8% for AML, OLM, VAL, and HCT, respectively. Consequently, the method was applied to the analysis of tablets and plasma of the patients using drugs including those substances.en
dc.description.sponsorshipBezmiâlem Vakıf Üniversitesi
dc.identifier10.5336/medsci.2008-8787
dc.identifier.citationTekkeli S. E. K. , -Development of an HPLC-UV Method for the Analysis of Drugs Used for Combined Hypertension Therapy in Pharmaceutical Preparations and Human Plasma-, JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, 2013
dc.identifier.pubmed23634320
dc.identifier.urihttps://hdl.handle.net/20.500.12645/4696
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleDevelopment of an HPLC-UV Method for the Analysis of Drugs Used for Combined Hypertension Therapy in Pharmaceutical Preparations and Human Plasma
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameTURKIYE KLINIKLERI TIP BILIMLERI DERGISI
local.avesis.id736b2efd-0567-434f-a83f-c5a9438cc277
local.avesis.response4566
local.org.facultyTıp Fakültesi
relation.isAuthorOfPublication837d5047-762e-43db-b252-9f3d1399378f
relation.isAuthorOfPublication.latestForDiscovery837d5047-762e-43db-b252-9f3d1399378f
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