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Andrographolide Exhibits Anticancer Potential Against Human Colon Cancer Cells by Inducing Cell Cycle Arrest and Programmed Cell Death via Augmentation of Intracellular Reactive Oxygen Species Level

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dc.contributor.authorKhan, IMRAN
dc.contributor.authorKhan, Fahad
dc.contributor.authorFarooqui, Arshi
dc.contributor.authorAnsari, Irfan A.
dc.contributor.institutionauthorKHAN, IMRAN
dc.date.accessioned2021-04-10T20:59:06Z
dc.date.available2021-04-10T20:59:06Z
dc.date.issued2018-01-01T00:00:00Z
dc.description.abstractAndrographolide, a diterpenoid lactone and a major constituent of Andrographis paniculata Nees, exhibits remarkable anticancer activity. However, the effect of andrographolide on colon cancer has not been completely elucidated yet. Thus, we investigated the chemopreventive potential of andrographolide in colon cancer HT-29 cells. The cytotoxic potential of andrographolide on HT-29 cells was determined by MTT assay, trypan blue exclusion assay, colony formation assay, and morphological analysis; and apoptotic property by DAPI and Hoechst staining, FITC-Annexin V assay, DNA fragmentation assay and caspase-3 activity assay. To elucidate andrographolide action, intracellular reactive oxygen species (ROS) level was determined by DCFDA dye; change in mitochondrial potential by Rhodamine123 and Mito Tracker Red CMXRos dye; and cell cycle modulatory property by flow cytometric analysis. Results of the study have shown that andrographolide decreased cell viability of HT-29 cells in a dose- and time-dependent manner. Furthermore, andrographolide induced apoptosis in HT-29 cells which seemed to be linked with augmented intracellular ROS level and disruption of mitochondrial membrane potential. Interestingly, andrographolide caused significant cell cycle arrest in G2/M phase at lower doses, but, in G0/G1 phase at higher doses. In summary, our results indicated that andrographolide exhibited antiproliferative and apoptotic properties against colon cancer HT-29 cells.
dc.identifier.citationKhan I., Khan F., Farooqui A., Ansari I. A. , -Andrographolide Exhibits Anticancer Potential Against Human Colon Cancer Cells by Inducing Cell Cycle Arrest and Programmed Cell Death via Augmentation of Intracellular Reactive Oxygen Species Level-, NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, cilt.70, sa.5, ss.787-803, 2018
dc.identifier.doi10.1080/01635581.2018.1470649
dc.identifier.scopus85047213601
dc.identifier.urihttp://hdl.handle.net/20.500.12645/28720
dc.identifier.wosWOS:000437722700009
dc.titleAndrographolide Exhibits Anticancer Potential Against Human Colon Cancer Cells by Inducing Cell Cycle Arrest and Programmed Cell Death via Augmentation of Intracellular Reactive Oxygen Species Level
dc.typeArticle
dspace.entity.typePublication
local.avesis.id21880cb9-121d-4010-b5c1-419ba437c516
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublication096be806-cf26-4766-b5b4-26f30c593af2
relation.isAuthorOfPublication.latestForDiscovery096be806-cf26-4766-b5b4-26f30c593af2
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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