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Real-time tracking, retrieval and gene expression analysis of migrating human T cells

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dc.contributor.authorMehling, Matthias
dc.contributor.authorFrank, Tino
dc.contributor.authorAlbayrak, CEM
dc.contributor.authorTay, Savas
dc.contributor.institutionauthorALBAYRAK, CEM
dc.date.accessioned2021-05-17T20:59:15Z
dc.date.available2021-05-17T20:59:15Z
dc.date.issued2015-01-01T00:00:00Z
dc.description.abstractDynamical analysis of single-cells allows assessment of the extent and role of cell-to-cell variability, however traditional dish-and-pipette techniques have hindered single-cell analysis in quantitative biology. We developed an automated microfluidic cell culture system that generates stable diffusion-based chemokine gradients, where cells can be placed in predetermined positions, monitored via single-cell time-lapse microscopy, and subsequently be retrieved based on their migration speed and directionality for further off-chip gene expression analysis, constituting a powerful platform for multiparameter quantitative studies of single-cell chemotaxis. Using this system we studied CXCL12-directed migration of individual human primary T cells. Spatiotemporally deterministic retrieval of T cell subsets in relation to their migration speed, and subsequent analysis with microfluidic droplet digital-PCR showed that the expression level of CXCR4 - the receptor of CXCL12 - underlies enhanced human T cell chemotaxis.
dc.identifier.citationMehling M., Frank T., Albayrak C., Tay S., -Real-time tracking, retrieval and gene expression analysis of migrating human T cells-, LAB ON A CHIP, cilt.15, sa.5, ss.1276-1283, 2015
dc.identifier.doi10.1039/c4lc01038h
dc.identifier.scopus84923163007
dc.identifier.urihttp://hdl.handle.net/20.500.12645/28889
dc.identifier.wosWOS:000349838200007
dc.titleReal-time tracking, retrieval and gene expression analysis of migrating human T cells
dc.typeArticle
dspace.entity.typePublication
local.avesis.ide1686dc2-9aa4-4790-a951-163bc40fe350
local.publication.isinternational1
relation.isAuthorOfPublicationced2804c-b9e5-4f25-9926-74404ade4f49
relation.isAuthorOfPublication.latestForDiscoveryced2804c-b9e5-4f25-9926-74404ade4f49
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